This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, L.
Right arrow Articles by Yang, Y. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, L.
Right arrow Articles by Yang, Y. C.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 07 1996, 3300-3307, Vol 16, No. 7
Copyright © 1996, American Society for Microbiology

Interleukin-11 mRNA stabilization in phorbol ester-stimulated primate bone marrow stromal cells

L Yang, CN Steussy, DK Fuhrer, J Hamilton and YC Yang
Department of Biochemistry and Molecular Biology, Walter Oncology Center, Indiana University School of Medicine, Indianapolis, 46202, USA.

12-O-Tetradecanoylphorbol-13-acetate (TPA) stimulation of PU-34 cells, a primate bone marrow stromal cell line, resulted in a prolonged elevation of interleukin-11 (IL-11) mRNA, which can be inhibited by protein synthesis inhibitors. Nuclear run-on assays and actinomycin D experiments demonstrated that the up-regulation of IL-11 gene expression is mainly controlled at the posttranscriptional level through the protein kinase C (PKC) pathway. Inhibition of PKC activity by calphostin C generated an IL-11 mRNA degradation intermediate in TPA- stimulated PU-34 cells. This intermediate retains the 5' untranslated region (5'UTR) and coding region of the IL-11 mRNA but has lost the poly(A) tail and the 3'UTR. The mechanisms underlying IL-11 mRNA stabilization were further investigated by transfections with a variety of chimeric IL-11 constructs and deletion mutants. Two important observations were made from these transient expression experiments: (i) the same 3'UTR of IL-11 mRNA shown to confer instability in one chimeric transcript may not function as a destabilizer in another chimeric RNA, and (ii) the 5'UTR, coding region, and 3'UTR all contribute to IL-11 mRNA decay, and labile IL-11 deletion transcripts are not necessarily stabilized by TPA stimulation. Our study suggests that multiple regions within the IL-11 mRNA are involved in TPA- stimulated IL-11 mRNA stabilization, possibly through a unique RNA folding conformation involving interactions of various RNA sequences within the IL-11 mRNA molecule.


This article has been cited by other articles:

  • Chou, Y.-T., Yang, Y.-C. (2006). Post-transcriptional Control of Cited2 by Transforming Growth Factor beta: REGULATION VIA SMADS AND CITED2 CODING REGION. J. Biol. Chem. 281: 18451-18462 [Abstract] [Full Text]  
  • Ming, X.-F., Stoecklin, G., Lu, M., Looser, R., Moroni, C. (2001). Parallel and Independent Regulation of Interleukin-3 mRNA Turnover by Phosphatidylinositol 3-Kinase and p38 Mitogen-Activated Protein Kinase. Mol. Cell. Biol. 21: 5778-5789 [Abstract] [Full Text]  
  • Chen, C.-Y., Gherzi, R., Andersen, J. S., Gaietta, G., Jürchott, K., Royer, H.-D., Mann, M., Karin, M. (2000). Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. Genes Dev. 14: 1236-1248 [Abstract] [Full Text]  
  • Ragheb, J. A., Deen, M., Schwartz, R. H. (1999). CD28-Mediated Regulation of mRNA Stability Requires Sequences Within the Coding Region of the IL-2 mRNA. J. Immunol. 163: 120-129 [Abstract] [Full Text]  
  • Dibbens, J. A., Miller, D. L., Damert, A., Risau, W., Vadas, M. A., Goodall, G. J. (1999). Hypoxic Regulation of Vascular Endothelial Growth Factor mRNA Stability Requires the Cooperation of Multiple RNA Elements. Mol. Biol. Cell 10: 907-919 [Abstract] [Full Text]  
  • Doktycz, M. J., Larimer, F. W., Pastrnak, M., Stevens, A. (1998). Comparative analyses of the secondary structures of synthetic and intracellular yeast MFA2 mRNAs. Proc. Natl. Acad. Sci. USA 95: 14614-14621 [Abstract] [Full Text]  
  • Du, X., Williams, D. A. (1997). Interleukin-11: Review of Molecular, Cell Biology, and Clinical Use. Blood 89: 3897-3908 [Full Text]  
  • Lai, W. S., Blackshear, P. J. (2001). Interactions of CCCH Zinc Finger Proteins with mRNA. TRISTETRAPROLIN-MEDIATED AU-RICH ELEMENT-DEPENDENT mRNA DEGRADATION CAN OCCUR IN THE ABSENCE OF A POLY(A) TAIL. J. Biol. Chem. 276: 23144-23154 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»