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Mol. Cell. Biol., 08 1996, 4349-4356, Vol 16, No. 8
Copyright © 1996, American Society for Microbiology

Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern

M Braunstein, RE Sobel, CD Allis, BM Turner and JR Broach
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.

Heterochromatin in metazoans induces transcriptional silencing, as exemplified by position effect variegation in Drosophila melanogaster and X-chromosome inactivation in mammals. Heterochromatic DNA is packaged in nucleosomes that are distinct in their acetylation pattern from those present in euchromatin, although the role these differences play in the structure of heterochromatin or in the effects of heterochromatin on transcriptional activity is unclear. Here we report that, as observed in the facultative heterochromatin of the inactive X chromosome in female mammalian cells, histones H3 and H4 in chromatin spanning the transcriptionally silenced mating-type cassettes of the yeast Saccharomyces cerevisiae are hypoacetylated relative to histones H3 and H4 of transcriptionally active regions of the genome. By immunoprecipitation of chromatin fragments with antibodies specific for H4 acetylated at particular lysine residues, we found that only three of the four lysine residues in the amino-terminal domain of histone H4 spanning the silent cassettes are hypoacetylated. Lysine 12 shows significant acetylation levels. This is identical to the pattern of histone H4 acetylation observed in centric heterochromatin of D. melanogaster. These two observations provide additional evidence that the silent cassettes are encompassed in the yeast equivalent of metazoan heterochromatin. Further, mutational analysis of the amino- terminal domain of histone H4 in S. cerevisiae demonstrated that this observed pattern of histone H4 acetylation is required for transcriptional silencing. This result, in conjunction with prior mutational analyses of yeast histones H3 and H4, indicates that the particular pattern of nucleosome acetylation found in heterochromatin is required for its effects on transcription and is not simply a side effect of heterochromatin formation.


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  • Bordoli, L., Husser, S., Luthi, U., Netsch, M., Osmani, H., Eckner, R. (2001). Functional analysis of the p300 acetyltransferase domain: the PHD finger of p300 but not of CBP is dispensable for enzymatic activity. Nucleic Acids Res 29: 4462-4471 [Abstract] [Full Text]  
  • Grozinger, C. M., Chao, E. D., Blackwell, H. E., Moazed, D., Schreiber, S. L. (2001). Identification of a Class of Small Molecule Inhibitors of the Sirtuin Family of NAD-dependent Deacetylases by Phenotypic Screening. J. Biol. Chem. 276: 38837-38843 [Abstract] [Full Text]  
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  • Gregory, R. I., Randall, T. E., Johnson, C. A., Khosla, S., Hatada, I., O'Neill, L. P., Turner, B. M., Feil, R. (2001). DNA Methylation Is Linked to Deacetylation of Histone H3, but Not H4, on the Imprinted Genes Snrpn and U2af1-rs1. Mol. Cell. Biol. 21: 5426-5436 [Abstract] [Full Text]  
  • Wang, Y., Vujcic, M., Kowalski, D. (2001). DNA Replication Forks Pause at Silent Origins near the HML Locus in Budding Yeast. Mol. Cell. Biol. 21: 4938-4948 [Abstract] [Full Text]  
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  • Deckert, J., Struhl, K. (2001). Histone Acetylation at Promoters Is Differentially Affected by Specific Activators and Repressors. Mol. Cell. Biol. 21: 2726-2735 [Abstract] [Full Text]  
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  • Sewack, G. F., Ellis, T. W., Hansen, U. (2001). Binding of TATA Binding Protein to a Naturally Positioned Nucleosome Is Facilitated by Histone Acetylation. Mol. Cell. Biol. 21: 1404-1415 [Abstract] [Full Text]  
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  • Li, Y.-C., Cheng, T.-H., Gartenberg, M. R. (2001). Establishment of Transcriptional Silencing in the Absence of DNA Replication. Science 291: 650-653 [Abstract] [Full Text]  
  • Bernstein, B. E., Tong, J. K., Schreiber, S. L. (2000). Genomewide studies of histone deacetylase function in yeast. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250477697v1 [Abstract] [Full Text]  
  • de Bruin, D., Kantrow, S. M., Liberatore, R. A., Zakian, V. A. (2000). Telomere Folding Is Required for the Stable Maintenance of Telomere Position Effects in Yeast. Mol. Cell. Biol. 20: 7991-8000 [Abstract] [Full Text]  
  • Jasencakova, Z., Meister, A., Walter, J., Turner, B. M., Schubert, I. (2000). Histone H4 Acetylation of Euchromatin and Heterochromatin Is Cell Cycle Dependent and Correlated with Replication Rather Than with Transcription. Plant Cell 12: 2087-2100 [Abstract] [Full Text]  
  • Lemon, B., Tjian, R. (2000). Orchestrated response: a symphony of transcription factors for gene control. Genes Dev. 14: 2551-2569 [Full Text]  
  • Kelly, T. J., Qin, S., Gottschling, D. E., Parthun, M. R. (2000). Type B Histone Acetyltransferase Hat1p Participates in Telomeric Silencing. Mol. Cell. Biol. 20: 7051-7058 [Abstract] [Full Text]  
  • Nissen, R. M., Yamamoto, K. R. (2000). The glucocorticoid receptor inhibits NFkappa B by interfering with serine-2 phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes Dev. 14: 2314-2329 [Abstract] [Full Text]  
  • Leach, T. J., Chotkowski, H. L., Wotring, M. G., Dilwith, R. L., Glaser, R. L. (2000). Replication of Heterochromatin and Structure of Polytene Chromosomes. Mol. Cell. Biol. 20: 6308-6316 [Abstract] [Full Text]  
  • Smith, J. S., Brachmann, C. B., Celic, I., Kenna, M. A., Muhammad, S., Starai, V. J., Avalos, J. L., Escalante-Semerena, J. C., Grubmeyer, C., Wolberger, C., Boeke, J. D. (2000). A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family. Proc. Natl. Acad. Sci. USA 97: 6658-6663 [Abstract] [Full Text]  
  • Guarente, L. (2000). Sir2 links chromatin silencing, metabolism, and aging. Genes Dev. 14: 1021-1026 [Full Text]  
  • Waterborg, J. H. (2000). Steady-state Levels of Histone Acetylation in Saccharomyces cerevisiae. J. Biol. Chem. 275: 13007-13011 [Abstract] [Full Text]  
  • Schübeler, D., Francastel, C., Cimbora, D. M., Reik, A., Martin, D. I.K., Groudine, M. (2000). Nuclear localization and histone acetylation: a pathway for chromatin opening and transcriptional activation of the human beta -globin locus. Genes Dev. 14: 940-950 [Abstract] [Full Text]  
  • IMAI, S., JOHNSON, F.B., MARCINIAK, R.A., MCVEY, M., PARK, P.U., GUARENTE, L. (2000). Sir2: An NAD-dependent Histone Deacetylase That Connects Chromatin Silencing, Metabolism, and Aging. Cold Spring Harb Symp Quant Biol 65: 297-302 [Abstract]  
  • Taddei, A., Roche, D., Sibarita, J.-B., Turner, B. M., Almouzni, G. (1999). Duplication and Maintenance of Heterochromatin Domains. JCB 147: 1153-1166 [Abstract] [Full Text]  
  • Bi, X., Braunstein, M., Shei, G.-J., Broach, J. R. (1999). The yeast HML I silencer defines a heterochromatin domain boundary by directional establishment of silencing. Proc. Natl. Acad. Sci. USA 96: 11934-11939 [Abstract] [Full Text]  
  • Eberharter, A., Sterner, D. E., Schieltz, D., Hassan, A., Yates, J. R. III, Berger, S. L., Workman, J. L. (1999). The ADA Complex Is a Distinct Histone Acetyltransferase Complex in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 6621-6631 [Abstract] [Full Text]  
  • Xu, E. Y., Kim, S., Replogle, K., Rine, J., Rivier, D. H. (1999). Identification of SAS4 and SAS5, Two Genes That Regulate Silencing in Saccharomyces cerevisiae. Genetics 153: 13-23 [Abstract] [Full Text]  
  • Sherman, J. M., Stone, E. M., Freeman-Cook, L. L., Brachmann, C. B., Boeke, J. D., Pillus, L. (1999). The Conserved Core of a Human SIR2 Homologue Functions in Yeast Silencing. Mol. Biol. Cell 10: 3045-3059 [Abstract] [Full Text]  
  • Sun, Z.-W., Hampsey, M. (1999). A General Requirement for the Sin3-Rpd3 Histone Deacetylase Complex in Regulating Silencing in Saccharomyces cerevisiae. Genetics 152: 921-932 [Abstract] [Full Text]  
  • Herrera, J. E., Sakaguchi, K., Bergel, M., Trieschmann, L., Nakatani, Y., Bustin, M. (1999). Specific Acetylation of Chromosomal Protein HMG-17 by PCAF Alters Its Interaction with Nucleosomes. Mol. Cell. Biol. 19: 3466-3473 [Abstract] [Full Text]  
  • Bi, X., Broach, J. R. (1999). UASrpg can function as a heterochromatin boundary element in yeast. Genes Dev. 13: 1089-1101 [Abstract] [Full Text]  
  • Smith, J. S., Caputo, E., Boeke, J. D. (1999). A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors. Mol. Cell. Biol. 19: 3184-3197 [Abstract] [Full Text]  
  • Venditti, S., Vega-Palas, M. A., Di Mauro, E. (1999). Heterochromatin Organization of a Natural Yeast Telomere. RECRUITMENT OF Sir3p THROUGH INTERACTION WITH HISTONE H4 N TERMINUS IS REQUIRED FOR THE ESTABLISHMENT OF REPRESSIVE STRUCTURES. J. Biol. Chem. 274: 1928-1933 [Abstract] [Full Text]  
  • Madisen, L., Krumm, A., Hebbes, T. R., Groudine, M. (1998). The Immunoglobulin Heavy Chain Locus Control Region Increases Histone Acetylation along Linked c-myc Genes. Mol. Cell. Biol. 18: 6281-6292 [Abstract] [Full Text]  
  • Waterborg, J. H. (1998). Dynamics of Histone Acetylation in Chlamydomonas reinhardtii. J. Biol. Chem. 273: 27602-27609 [Abstract] [Full Text]  
  • Kadosh, D., Struhl, K. (1998). Targeted Recruitment of the Sin3-Rpd3 Histone Deacetylase Complex Generates a Highly Localized Domain of Repressed Chromatin In Vivo. Mol. Cell. Biol. 18: 5121-5127 [Abstract] [Full Text]  
  • Selker, E. U. (1998). Trichostatin A causes selective loss of DNA methylation in Neurospora. Proc. Natl. Acad. Sci. USA 95: 9430-9435 [Abstract] [Full Text]  
  • Smith, J. S., Brachmann, C. B., Pillus, L., Boeke, J. D. (1998). Distribution of a Limited Sir2 Protein Pool Regulates the Strength of Yeast rDNA Silencing and Is Modulated by Sir4p. Genetics 149: 1205-1219 [Abstract] [Full Text]  
  • Walia, H., Chen, H. Y., Sun, J.-M., Holth, L. T., Davie, J. R. (1998). Histone Acetylation Is Required to Maintain the Unfolded Nucleosome Structure Associated with Transcribing DNA. J. Biol. Chem. 273: 14516-14522 [Abstract] [Full Text]  
  • Ruiz-Garcia, A. B., Sendra, R., Galiana, M., Pamblanco, M., Perez-Ortin, J. E., Tordera, V. (1998). HAT1 and HAT2 Proteins Are Components of a Yeast Nuclear Histone Acetyltransferase Enzyme Specific for Free Histone H4. J. Biol. Chem. 273: 12599-12605 [Abstract] [Full Text]  
  • Smith, E. R., Eisen, A., Gu, W., Sattah, M., Pannuti, A., Zhou, J., Cook, R. G., Lucchesi, J. C., Allis, C. D. (1998). ESA1 is a histone acetyltransferase that is essential for growth in yeast. Proc. Natl. Acad. Sci. USA 95: 3561-3565 [Abstract] [Full Text]  
  • Struhl, K. (1998). Histone acetylation and transcriptional regulatory mechanisms. Genes Dev. 12: 599-606 [Full Text]  
  • Kuo, M.-H., Zhou, J., Jambeck, P., Churchill, M. E.A., Allis, C. D. (1998). Histone acetyltransferase activity of yeast Gcn5p is required for the activation of target genes in vivo. Genes Dev. 12: 627-639 [Abstract] [Full Text]  
  • Enomoto, S., Berman, J. (1998). Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev. 12: 219-232 [Abstract] [Full Text]  
  • COCKELL, M., GOTTA, M., PALLADINO, F., MARTIN, S.G., GASSER, S.M. (1998). Targeting Sir Proteins to Sites of Action: A General Mechanism for Regulated Repression. Cold Spring Harb Symp Quant Biol 63: 401-412 [Abstract]  
  • WAHI, M., KOMACHI, K., JOHNSON, A.D. (1998). Gene Regulation by the Yeast Ssn6-Tup1 Corepressor. Cold Spring Harb Symp Quant Biol 63: 447-458 [Abstract]  
  • DUTNALL, R.N., TAFROV, S.T., STERNGLANZ, R., RAMAKRISHNAN, V. (1998). Structure of the Yeast Histone Acetyltransferase Hat1: Insights into Substrate Specificity and Implications for the Gcn5-related N-acetyltransferase Superfamily. Cold Spring Harb Symp Quant Biol 63: 501-508 [Abstract]  
  • Monson, E. K., de Bruin, D., Zakian, V. A. (1997). The yeast Cac1 protein is required for the stable inheritance of transcriptionally repressed chromatin at telomeres. Proc. Natl. Acad. Sci. USA 94: 13081-13086 [Abstract] [Full Text]  
  • Wu, C. (1997). Chromatin Remodeling and the Control of Gene Expression. J. Biol. Chem. 272: 28171-28174 [Full Text]  
  • Enomoto, S, McCune-Zierath, P D, Gerami-Nejad, M, Sanders, M A, Berman, J (1997). RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo.. Genes Dev. 11: 358-370 [Abstract]  
  • Smith, J S, Boeke, J D (1997). An unusual form of transcriptional silencing in yeast ribosomal DNA.. Genes Dev. 11: 241-254 [Abstract]  
  • Bryk, M, Banerjee, M, Murphy, M, Knudsen, K E, Garfinkel, D J, Curcio, M J (1997). Transcriptional silencing of Ty1 elements in the RDN1 locus of yeast.. Genes Dev. 11: 255-269 [Abstract]  
  • Smothers, J., Mizzen, C., Tubbert, M., Cook, R., Allis, C. (1997). Pdd1p associates with germline-restricted chromatin and a second novel anlagen-enriched protein in developmentally programmed DNA elimination structures. Development 124: 4537-4545 [Abstract]  
  • Rundlett, S. E., Carmen, A. A., Kobayashi, R., Bavykin, S., Turner, B. M., Grunstein, M. (1996). HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription. Proc. Natl. Acad. Sci. USA 93: 14503-14508 [Abstract] [Full Text]  
  • Zhao, T., Heyduk, T., Allis, C. D., Eissenberg, J. C. (2000). Heterochromatin Protein 1 Binds to Nucleosomes and DNA in Vitro. J. Biol. Chem. 275: 28332-28338 [Abstract] [Full Text]  
  • Bone, J. R., Roth, S. Y. (2001). Recruitment of the Yeast Tup1p-Ssn6p Repressor Is Associated with Localized Decreases in Histone Acetylation. J. Biol. Chem. 276: 1808-1813 [Abstract] [Full Text]  
  • Bacolla, A., Pradhan, S., Larson, J. E., Roberts, R. J., Wells, R. D. (2001). Recombinant Human DNA (Cytosine-5) Methyltransferase. III. ALLOSTERIC CONTROL, REACTION ORDER, AND INFLUENCE OF PLASMID TOPOLOGY AND TRIPLET REPEAT LENGTH ON METHYLATION OF THE FRAGILE X CGG{middle dot}CCG SEQUENCE. J. Biol. Chem. 276: 18605-18613 [Abstract] [Full Text]  
  • Loewith, R., Smith, J. S., Meijer, M., Williams, T. J., Bachman, N., Boeke, J. D., Young, D. (2001). Pho23 Is Associated with the Rpd3 Histone Deacetylase and Is Required for Its Normal Function in Regulation of Gene Expression and Silencing in Saccharomyces cerevisiae. J. Biol. Chem. 276: 24068-24074 [Abstract] [Full Text]  
  • Bernstein, B. E., Tong, J. K., Schreiber, S. L. (2000). Genomewide studies of histone deacetylase function in yeast. Proc. Natl. Acad. Sci. USA 97: 13708-13713 [Abstract] [Full Text]  
  • Armstrong, C. M., Kaeberlein, M., Imai, S. I., Guarente, L. (2002). Mutations in Saccharomyces cerevisiae Gene SIR2 Can Have Differential Effects on In Vivo Silencing Phenotypes and In Vitro Histone Deacetylation Activity. Mol. Biol. Cell 13: 1427-1438 [Abstract] [Full Text]  


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