This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schulze, A.
Right arrow Articles by Henglein, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schulze, A.
Right arrow Articles by Henglein, B.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Sep 1996, 4632-4638, Vol 16, No. 9
Copyright © 1996, American Society for Microbiology

Anchorage-dependent transcription of the cyclin A gene

A Schulze, K Zerfass-Thome, J Berges, S Middendorp, P Jansen-Durr and B Henglein
Forschungschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

NIH 3T3 cells cultured in suspension fail to express cyclin A and hence cannot enter S phase and divide. We show that loss of cell adhesion to substratum abrogates cyclin A gene expression by blocking its promoter activity through the E2F site that mediates its cell cycle regulation in adherent cells. In suspended cells, G0-specific E2F complexes remain bound to the cyclin A promoter. Overexpression of cyclin D1 restores cyclin A transcription in suspended cells and rescues them from cell cycle arrest. In suspended cells, cyclin D1 and cyclin E accumulate normally upon serum stimulation, but their associated kinases remain inactive; their substrates, pRb and p107, are not hyperphosphorylated. Concomitantly, the cyclin-dependent kinase inhibitor, p27KIP1, is stabilized. Ectopic expression of p27KIP1 blocks cyclin A promoter activity through its EN binding site. These data suggest that the block to cyclin A transcription in nonadherent NIH 3T3 cells results from stabilization of p27KIP1 and subsequent inactivation of the specific E2F moiety required for its induction.


This article has been cited by other articles:

  • Mori, K., Hirao, E., Toya, Y., Oshima, Y., Ishikawa, F., Nose, K., Shibanuma, M. (2009). Competitive Nuclear Export of Cyclin D1 and Hic-5 Regulates Anchorage Dependence of Cell Growth and Survival. Mol. Biol. Cell 20: 218-232 [Abstract] [Full Text]  
  • Margadant, C., van Opstal, A., Boonstra, J. (2007). Focal adhesion signaling and actin stress fibers are dispensable for progression through the ongoing cell cycle. J. Cell Sci. 120: 66-76 [Abstract] [Full Text]  
  • Engedal, N., Gjevik, T., Blomhoff, R., Blomhoff, H. K. (2006). All-trans Retinoic Acid Stimulates IL-2-Mediated Proliferation of Human T Lymphocytes: Early Induction of Cyclin D3.. J. Immunol. 177: 2851-2861 [Abstract] [Full Text]  
  • Bryant, P., Zheng, Q., Pumiglia, K. (2006). Focal Adhesion Kinase Controls Cellular Levels of p27/Kip1 and p21/Cip1 through Skp2-Dependent and -Independent Mechanisms.. Mol. Cell. Biol. 26: 4201-4213 [Abstract] [Full Text]  
  • Manne, U., Jhala, N. C., Jones, J., Weiss, H. L., Chatla, C., Meleth, S., Suarez-Cuervo, C., Grizzle, W. E. (2004). Prognostic Significance of p27kip-1 Expression in Colorectal Adenocarcinomas Is Associated with Tumor Stage. Clin. Cancer Res. 10: 1743-1752 [Abstract] [Full Text]  
  • Hauser, P., Ma, L., Agrawal, D., Haura, E., Cress, W. D., Pledger, W. J. (2004). Efficient Down-Regulation of Cyclin A-Associated Activity and Expression in Suspended Primary Keratinocytes Requires p21Cip1. Mol Cancer Res 2: 96-104 [Abstract] [Full Text]  
  • Fassett, J. T., Tobolt, D., Nelsen, C. J., Albrecht, J. H., Hansen, L. K. (2003). The Role of Collagen Structure in Mitogen Stimulation of ERK, Cyclin D1 Expression, and G1-S Progression in Rat Hepatocytes. J. Biol. Chem. 278: 31691-31700 [Abstract] [Full Text]  
  • Uttamsingh, S., Zong, C. S., Wang, L.-H. (2003). Matrix-independent Activation of Phosphatidylinositol 3-Kinase, Stat3, and Cyclin A-associated Cdk2 Is Essential for Anchorage-independent Growth of v-Ros-transformed Chicken Embryo Fibroblasts. J. Biol. Chem. 278: 18798-18810 [Abstract] [Full Text]  
  • Zhao, J., Pestell, R., Guan, J.-L. (2001). Transcriptional Activation of Cyclin D1 Promoter by FAK Contributes to Cell Cycle Progression. Mol. Biol. Cell 12: 4066-4077 [Abstract] [Full Text]  
  • Bottazzi, M. E., Buzzai, M., Zhu, X., Desdouets, C., Brechot, C., Assoian, R. K. (2001). Distinct Effects of Mitogens and the Actin Cytoskeleton on CREB and Pocket Protein Phosphorylation Control the Extent and Timing of Cyclin A Promoter Activity. Mol. Cell. Biol. 21: 7607-7616 [Abstract] [Full Text]  
  • Klekotka, P. A., Santoro, S. A., Ho, A., Dowdy, S. F., Zutter, M. M. (2001). Mammary Epithelial Cell-Cycle Progression via the {alpha}2{beta}1 Integrin : Unique and Synergistic Roles of the {alpha}2 Cytoplasmic Domain. Am. J. Pathol. 159: 983-992 [Abstract] [Full Text]  
  • Carrano, A. C., Pagano, M. (2001). Role of the F-Box Protein Skp2 in Adhesion-Dependent Cell Cycle Progression. JCB 153: 1381-1390 [Abstract] [Full Text]  
  • Bazarov, A. V., Adachi, S., Li, S.-F., Mateyak, M. K., Wei, S., Sedivy, J. M. (2001). A Modest Reduction in c-Myc Expression Has Minimal Effects on Cell Growth and Apoptosis But Dramatically Reduces Susceptibility to Ras and Raf Transformation. Cancer Res. 61: 1178-1186 [Abstract] [Full Text]  
  • Solorzano, L., Rieber, M. S., Rieber, M. (2000). Lower Cyclin H and Cyclin-dependent Kinase-activating Kinase Activity in Cell Cycle Arrest Induced by Lack of Adhesion to Substratum. Cancer Res. 60: 7114-7118 [Abstract] [Full Text]  
  • Dannenberg, J.-H., van Rossum, A., Schuijff, L., te Riele, H. (2000). Ablation of the Retinoblastoma gene family deregulates G1 control causing immortalization and increased cell turnover under growth-restricting conditions. Genes Dev. 14: 3051-3064 [Abstract] [Full Text]  
  • WILKINSON, M. G., MILLAR, J. B. A. (2000). Control of the eukaryotic cell cycle by MAP kinase signaling pathways. FASEB J. 14: 2147-2157 [Abstract] [Full Text]  
  • Howard, C. M., Claudio, P. P., Antonio De Luca, , Stiegler, P., Jori, F. P., Safdar, N. M., Caputi, M., Khalili, K., Giordano, A. (2000). Inducible pRb2/p130 Expression and Growth-suppressive Mechanisms: Evidence of a pRb2/p130, p27Kip1, and Cyclin E Negative Feedback Regulatory Loop. Cancer Res. 60: 2737-2744 [Abstract] [Full Text]  
  • Zhu, X., Scharf, E., Assoian, R. K. (2000). Induction of Anchorage-independent Growth by Transforming Growth Factor-beta Linked to Anchorage-independent Expression of Cyclin D1. J. Biol. Chem. 275: 6703-6706 [Abstract] [Full Text]  
  • Philips, A., Roux, P., Coulon, V., Bellanger, J.-M., Vie, A., Vignais, M.-L., Blanchard, J. M. (2000). Differential Effect of Rac and Cdc42 on p38 Kinase Activity and Cell Cycle Progression of Nonadherent Primary Mouse Fibroblasts. J. Biol. Chem. 275: 5911-5917 [Abstract] [Full Text]  
  • Takano, Y., Kato, Y., van Diest, P. J., Masuda, M., Mitomi, H., Okayasu, I. (2000). Cyclin D2 Overexpression and Lack of p27 Correlate Positively and Cyclin E Inversely with a Poor Prognosis in Gastric Cancer Cases. Am. J. Pathol. 156: 585-594 [Abstract] [Full Text]  
  • Jiang, Y., Prosper, F., Verfaillie, C. M. (2000). Opposing effects of engagement of integrins and stimulation of cytokine receptors on cell cycle progression of normal human hematopoietic progenitors. Blood 95: 846-854 [Abstract] [Full Text]  
  • Moiseeva, E. P, Javed, Q., Spring, E. L, de Bono, D. P (2000). Galectin 1 is involved in vascular smooth muscle cell proliferation. Cardiovasc Res 45: 493-502 [Abstract] [Full Text]  
  • Asthagiri, A., Reinhart, C., Horwitz, A., Lauffenburger, D. (2000). The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis. J. Cell Sci. 113: 4499-4510 [Abstract]  
  • Basu, A., Rodeck, U., Prendergast, G. C., Howe, C. C. (1999). Loss of Insulin-like Growth Factor I Receptor-dependent Expression of p107 and Cyclin A in Cells That Lack the Extracellular Matrix Protein Secreted Protein Acidic and Rich in Cysteine. Cell Growth Differ. 10: 721-728 [Abstract] [Full Text]  
  • Bottazzi, M. E., Zhu, X., Bohmer, R. M., Assoian, R. K. (1999). Regulation of P21cip1 Expression by Growth Factors and the Extracellular Matrix Reveals a Role for Transient ERK Activity in G1 Phase. JCB 146: 1255-1264 [Abstract] [Full Text]  
  • Chen, D., Guo, K., Yang, J., Frazier, W. A., Isner, J. M., Andres, V. (1999). Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1. Am. J. Physiol. Heart Circ. Physiol. 277: H1100-H1106 [Abstract] [Full Text]  
  • Vogt, B., Zerfaß-Thome, K., Schulze, A., Botz, J. W., Zwerschke, W., Jansen-Dürr, P. (1999). Regulation of cyclin E gene expression by the human papillomavirus type 16 E7 oncoprotein. J. Gen. Virol. 80: 2103-2113 [Abstract] [Full Text]  
  • Duro, D, Schulze, A, Vogt, B, Bartek, J, Mittnacht, S, Jansen-Durr, P (1999). Activation of cyclin A gene expression by the cyclin encoded by human herpesvirus-8. J. Gen. Virol. 80: 549-555 [Abstract]  
  • Davey, G, Buzzai, M, Assoian, R. (1999). Reduced expression of (alpha)5(beta)1 integrin prevents spreading-dependent cell proliferation. J. Cell Sci. 112: 4663-4672 [Abstract]  
  • Christopher, R., Judge, S., Vincent, P., Higgins, P., McKeown-Longo, P. (1999). The amino-terminal matrix assembly domain of fibronectin stabilizes cell shape and prevents cell cycle progression. J. Cell Sci. 112: 3225-3235 [Abstract]  
  • Hansen, L., Albrecht, J. (1999). Regulation of the hepatocyte cell cycle by type I collagen matrix: role of cyclin D1. J. Cell Sci. 112: 2971-2981 [Abstract]  
  • Huang, S., Chen, C. S., Ingber, D. E. (1998). Control of Cyclin D1, p27Kip1, and Cell Cycle Progression in Human Capillary Endothelial Cells by Cell Shape and Cytoskeletal Tension. Mol. Biol. Cell 9: 3179-3193 [Abstract] [Full Text]  
  • Thomas, G. V., Szigeti, K., Murphy, M., Draetta, G., Pagano, M., Loda, M. (1998). Down-Regulation of p27 Is Associated with Development of Colorectal Adenocarcinoma Metastases. Am. J. Pathol. 153: 681-687 [Abstract] [Full Text]  
  • Kirkham, P. A., Lam, E. W.-F., Takamatsu, H.-H., Michael, R., Parkhouse, E. (1998). Transcription Factor E2F Controls the Reversible {gamma}{delta} T Cell Growth Arrest Mediated Through WC1. J. Immunol. 161: 1630-1636 [Abstract] [Full Text]  
  • Aplin, A. E., Howe, A., Alahari, S. K., Juliano, R. L. (1998). Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins. Pharmacol. Rev. 50: 197-264 [Abstract] [Full Text]  
  • Yang, J.-J., Kang, J.-S., Krauss, R. S. (1998). Ras Signals to the Cell Cycle Machinery via Multiple Pathways To Induce Anchorage-Independent Growth. Mol. Cell. Biol. 18: 2586-2595 [Abstract] [Full Text]  
  • Schulze, A., Mannhardt, B., Zerfass-Thome, K., Zwerschke, W., Jansen-Durr, P. (1998). Anchorage-Independent Transcription of the Cyclin A Gene Induced by the E7 Oncoprotein of Human Papillomavirus Type 16. J. Virol. 72: 2323-2334 [Abstract] [Full Text]  
  • Harvat, B., Wang, A, Seth, P, Jetten, A. (1998). Up-regulation of p27Kip1, p21WAF1/Cip1 and p16Ink4a is associated with, but not sufficient for, induction of squamous differentiation. J. Cell Sci. 111: 1185-1196 [Abstract]  
  • Wu, R.-C., Schonthal, A. H. (1997). Activation of p53-p21waf1 Pathway in Response to Disruption of Cell-Matrix Interactions. J. Biol. Chem. 272: 29091-29098 [Abstract] [Full Text]  
  • Hurford, R K, Cobrinik, D, Lee, M H, Dyson, N (1997). pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes.. Genes Dev. 11: 1447-1463 [Abstract]  
  • Radeva, G., Petrocelli, T., Behrend, E., Leung-Hagesteijn, C., Filmus, J., Slingerland, J., Dedhar, S. (1997). Overexpression of the Integrin-linked Kinase Promotes Anchorage-independent Cell Cycle Progression. J. Biol. Chem. 272: 13937-13944 [Abstract] [Full Text]  
  • Assoian, R. K. (1997). Anchorage-dependent Cell Cycle Progression. JCB 136: 1-4 [Full Text]  
  • Chakravarthy, M. V., Abraha, T. W., Schwartz, R. J., Fiorotto, M. L., Booth, F. W. (2000). Insulin-like Growth Factor-I Extends in Vitro Replicative Life Span of Skeletal Muscle Satellite Cells by Enhancing G1/S Cell Cycle Progression via the Activation of Phosphatidylinositol 3'-Kinase/Akt Signaling Pathway. J. Biol. Chem. 275: 35942-35952 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»