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Mol. Cell. Biol., Jan 1997, 469-481, Vol 17, No. 1
Copyright © 1997, American Society for Microbiology

HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator

A Nakai, M Tanabe, Y Kawazoe, J Inazawa, RI Morimoto and K Nagata
Department of Cell Biology, Kyoto University, Japan. nakai@chest.kyoto- u.ac.jp

Heat shock transcription factors (HSFs) mediate the inducible transcriptional response of genes that encode heat shock proteins and molecular chaperones. In vertebrates, three related HSF genes (HSF1 to - 3) and the respective gene products (HSFs) have been characterized. We report the cloning and characterization of human HSF4 (hHSF4), a novel member of the hHSF family that shares properties with other members of the HSF family yet appears to be functionally distinct. hHSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. hHSF4 is preferentially expressed in the human heart, brain, skeletal muscle, and pancreas. Transient transfection of hHSF4 in HeLa cells, which do not express hHSF4, results in a constitutively active DNA binding trimer which, unlike other members of the HSF family, lacks the properties of a transcriptional activator. Constitutive overexpression of hHSF4 in HeLa cells results in reduced expression of the endogenous hsp70, hsp90, and hsp27 genes. hHSF4 represents a novel hHSF that exhibits tissue- specific expression and functions to repress the expression of genes encoding heat shock proteins and molecular chaperones.


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  • Satyal, S. H., Chen, D., Fox, S. G., Kramer, J. M., Morimoto, R. I. (1998). Negative regulation of the heat shock transcriptional response by HSBP1. Genes Dev. 12: 1962-1974 [Abstract] [Full Text]  
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  • Farkas, T., Kutskova, Y. A., Zimarino, V. (1998). Intramolecular Repression of Mouse Heat Shock Factor 1. Mol. Cell. Biol. 18: 906-918 [Abstract] [Full Text]  
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  • Leppa, S., Pirkkala, L., Chow, S. C., Eriksson, J. E., Sistonen, L. (1997). Thioredoxin Is Transcriptionally Induced upon Activation of Heat Shock Factor 2. J. Biol. Chem. 272: 30400-30404 [Abstract] [Full Text]  
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  • Tanabe, M., Nakai, A., Kawazoe, Y., Nagata, K. (1997). Different Thresholds in the Responses of Two Heat Shock Transcription Factors, HSF1 and HSF3. J. Biol. Chem. 272: 15389-15395 [Abstract] [Full Text]  
  • Rallu, M., Loones, Mt., Lallemand, Y., Morimoto, R., Morange, M., Mezger, V. (1997). Function and regulation of heat shock factor 2 during mouse embryogenesis. Proc. Natl. Acad. Sci. USA 94: 2392-2397 [Abstract] [Full Text]  
  • Cotto, J, Fox, S, Morimoto, R (1997). HSF1 granules: a novel stress-induced nuclear compartment of human cells. J. Cell Sci. 110: 2925-2934 [Abstract]  
  • Somasundaram, T., Bhat, S. P. (2000). Canonical Heat Shock Element in the alpha B-crystallin Gene Shows Tissue-specific and Developmentally Controlled Interactions with Heat Shock Factor. J. Biol. Chem. 275: 17154-17159 [Abstract] [Full Text]  
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