This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paidhungat, M.
Right arrow Articles by Garrett, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paidhungat, M.
Right arrow Articles by Garrett, S.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Nov 1997, 6339-6347, Vol 17, No. 11
Copyright © 1997, American Society for Microbiology

A homolog of mammalian, voltage-gated calcium channels mediates yeast pheromone-stimulated Ca2+ uptake and exacerbates the cdc1(Ts) growth defect

M Paidhungat and S Garrett
Department of Molecular Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.

Previous studies attributed the yeast (Saccharomyces cerevisiae) cdc1(Ts) growth defect to loss of an Mn2+-dependent function. In this report we show that cdc1(Ts) temperature-sensitive growth is also associated with an increase in cytosolic Ca2+. We identified two recessive suppressors of the cdc1(Ts) temperature-sensitive growth which block Ca2+ uptake and accumulation, suggesting that cytosolic Ca2+ exacerbates or is responsible for the cdc1(Ts) growth defect. One of the cdc1(Ts) suppressors is identical to a gene, MID1, recently implicated in mating pheromone-stimulated Ca2+ uptake. The gene (CCH1) corresponding to the second suppressor encodes a protein that bears significant sequence similarity to the pore-forming subunit (alpha1) of plasma membrane, voltage-gated Ca2+ channels from higher eukaryotes. Strains lacking Mid1 or Cch1 protein exhibit a defect in pheromone- induced Ca2+ uptake and consequently lose viability upon mating arrest. The mid1delta and cch1delta mutants also display reduced tolerance to monovalent cations such as Li+, suggesting a role for Ca2+ uptake in the calcineurin-dependent ion stress response. Finally, mid1delta cch1delta double mutants are, by both physiological and genetic criteria, identical to single mutants. These and other results suggest Mid1 and Cch1 are components of a yeast Ca2+ channel that may mediate Ca2+ uptake in response to mating pheromone, salt stress, and Mn2+ depletion.


This article has been cited by other articles:

  • Teng, J., Goto, R., Iida, K., Kojima, I., Iida, H. (2008). Ion-channel blocker sensitivity of voltage-gated calcium-channel homologue Cch1 in Saccharomyces cerevisiae. Microbiology 154: 3775-3781 [Abstract] [Full Text]  
  • Martinac, B., Saimi, Y., Kung, C. (2008). Ion Channels in Microbes. Physiol. Rev. 88: 1449-1490 [Abstract] [Full Text]  
  • Campos, C. B. L., Di Benedette, J. P. T., Morais, F. V., Ovalle, R., Nobrega, M. P. (2008). Evidence for the Role of Calcineurin in Morphogenesis and Calcium Homeostasis during Mycelium-to-Yeast Dimorphism of Paracoccidioides brasiliensis. Eukaryot Cell 7: 1856-1864 [Abstract] [Full Text]  
  • Losev, E., Papanikou, E., Rossanese, O. W., Glick, B. S. (2008). Cdc1p Is an Endoplasmic Reticulum-Localized Putative Lipid Phosphatase That Affects Golgi Inheritance and Actin Polarization by Activating Ca2+ Signaling. Mol. Cell. Biol. 28: 3336-3343 [Abstract] [Full Text]  
  • Lew, R. R., Abbas, Z., Anderca, M. I., Free, S. J. (2008). Phenotype of a Mechanosensitive Channel Mutant, mid-1, in a Filamentous Fungus, Neurospora crassa. Eukaryot Cell 7: 647-655 [Abstract] [Full Text]  
  • Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H. (2007). Essential, Completely Conserved Glycine Residue in the Domain III S2 S3 Linker of Voltage-gated Calcium Channel {alpha}1 Subunits in Yeast and Mammals. J. Biol. Chem. 282: 25659-25667 [Abstract] [Full Text]  
  • Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida, H. (2007). Arabidopsis plasma membrane protein crucial for Ca2+ influx and touch sensing in roots. Proc. Natl. Acad. Sci. USA 104: 3639-3644 [Abstract] [Full Text]  
  • Liu, M., Du, P., Heinrich, G., Cox, G. M., Gelli, A. (2006). Cch1 Mediates Calcium Entry in Cryptococcus neoformans and Is Essential in Low-Calcium Environments. Eukaryot Cell 5: 1788-1796 [Abstract] [Full Text]  
  • Zhang, N.-N., Dudgeon, D. D., Paliwal, S., Levchenko, A., Grote, E., Cunningham, K. W. (2006). Multiple Signaling Pathways Regulate Yeast Cell Death during the Response to Mating Pheromones. Mol. Biol. Cell 17: 3409-3422 [Abstract] [Full Text]  
  • Wang, Y.-J., Yu, J.-N., Chen, T., Zhang, Z.-G., Hao, Y.-J., Zhang, J.-S., Chen, S.-Y. (2005). Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat. J Exp Bot 56: 3051-3060 [Abstract] [Full Text]  
  • Noma, S., Iida, K., Iida, H. (2005). Polarized Morphogenesis Regulator Spa2 Is Required for the Function of Putative Stretch-Activated Ca2+-Permeable Channel Component Mid1 in Saccharomyces cerevisiae. Eukaryot Cell 4: 1353-1363 [Abstract] [Full Text]  
  • Levin, D. E. (2005). Cell Wall Integrity Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 262-291 [Abstract] [Full Text]  
  • Viladevall, L., Serrano, R., Ruiz, A., Domenech, G., Giraldo, J., Barcelo, A., Arino, J. (2004). Characterization of the Calcium-mediated Response to Alkaline Stress in Saccharomyces cerevisiae. J. Biol. Chem. 279: 43614-43624 [Abstract] [Full Text]  
  • Ton, V.-K., Rao, R. (2004). Functional expression of heterologous proteins in yeast: insights into Ca2+ signaling and Ca2+-transporting ATPases. Am. J. Physiol. Cell Physiol. 287: C580-C589 [Abstract] [Full Text]  
  • Spelbrink, R. G., Dilmac, N., Allen, A., Smith, T. J., Shah, D. M., Hockerman, G. H. (2004). Differential Antifungal and Calcium Channel-Blocking Activity among Structurally Related Plant Defensins. Plant Physiol. 135: 2055-2067 [Abstract] [Full Text]  
  • Kaur, R., Castano, I., Cormack, B. P. (2004). Functional Genomic Analysis of Fluconazole Susceptibility in the Pathogenic Yeast Candida glabrata: Roles of Calcium Signaling and Mitochondria. Antimicrob. Agents Chemother. 48: 1600-1613 [Abstract] [Full Text]  
  • Eguez, L., Chung, Y.-S., Kuchibhatla, A., Paidhungat, M., Garrett, S. (2004). Yeast Mn2+ Transporter, Smf1p, Is Regulated by Ubiquitin-Dependent Vacuolar Protein Sorting. Genetics 167: 107-117 [Abstract] [Full Text]  
  • Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida, H. (2004). Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1. Plant Cell Physiol 45: 496-500 [Abstract] [Full Text]  
  • Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G. E., Read, N. D., Seiler, S., Bell-Pedersen, D., Paietta, J., Plesofsky, N., Plamann, M., Goodrich-Tanrikulu, M., Schulte, U., Mannhaupt, G., Nargang, F. E., Radford, A., Selitrennikoff, C., Galagan, J. E., Dunlap, J. C., Loros, J. J., Catcheside, D., Inoue, H., Aramayo, R., Polymenis, M., Selker, E. U., Sachs, M. S., Marzluf, G. A., Paulsen, I., Davis, R., Ebbole, D. J., Zelter, A., Kalkman, E. R., O'Rourke, R., Bowring, F., Yeadon, J., Ishii, C., Suzuki, K., Sakai, W., Pratt, R. (2004). Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68: 1-108 [Abstract] [Full Text]  
  • Muller, E. M., Mackin, N. A., Erdman, S. E., Cunningham, K. W. (2003). Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae. J. Biol. Chem. 278: 38461-38469 [Abstract] [Full Text]  
  • Bonilla, M., Cunningham, K. W. (2003). Mitogen-activated Protein Kinase Stimulation of Ca2+ Signaling Is Required for Survival of Endoplasmic Reticulum Stress in Yeast. Mol. Biol. Cell 14: 4296-4305 [Abstract] [Full Text]  
  • Gupta, S. S., Ton, V.-K., Beaudry, V., Rulli, S., Cunningham, K., Rao, R. (2003). Antifungal Activity of Amiodarone Is Mediated by Disruption of Calcium Homeostasis. J. Biol. Chem. 278: 28831-28839 [Abstract] [Full Text]  
  • Tada, T., Ohmori, M., Iida, H. (2003). Molecular Dissection of the Hydrophobic Segments H3 and H4 of the Yeast Ca2+ Channel Component Mid1. J. Biol. Chem. 278: 9647-9654 [Abstract] [Full Text]  
  • Matsumoto, T. K., Ellsmore, A. J., Cessna, S. G., Low, P. S., Pardo, J. M., Bressan, R. A., Hasegawa, P. M. (2002). An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae. J. Biol. Chem. 277: 33075-33080 [Abstract] [Full Text]  
  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
  • Bonilla, M., Cunningham, K. W. (2002). Calcium Release and Influx in Yeast: TRPC and VGCC Rule Another Kingdom. Sci Signal 2002: pe17-pe17 [Abstract] [Full Text]  
  • Maruoka, T., Nagasoe, Y., Inoue, S., Mori, Y., Goto, J., Ikeda, M., Iida, H. (2002). Essential Hydrophilic Carboxyl-terminal Regions Including Cysteine Residues of the Yeast Stretch-activated Calcium-permeable Channel Mid1. J. Biol. Chem. 277: 11645-11652 [Abstract] [Full Text]  
  • Muller, E. M., Locke, E. G., Cunningham, K. W. (2001). Differential Regulation of Two Ca2+ Influx Systems by Pheromone Signaling in Saccharomyces cerevisiae. Genetics 159: 1527-1538 [Abstract] [Full Text]  
  • Palmer, C. P., Zhou, X.-L., Lin, J., Loukin, S. H., Kung, C., Saimi, Y. (2001). A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca2+-permeable channel in the yeast vacuolar membrane. Proc. Natl. Acad. Sci. USA 10.1073/pnas.141036198v1 [Abstract] [Full Text]  
  • Abe, F., Horikoshi, K. (2000). Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 8093-8102 [Abstract] [Full Text]  
  • Rusnak, F., Mertz, P. (2000). Calcineurin: Form and Function. Physiol. Rev. 80: 1483-1521 [Abstract] [Full Text]  
  • Locke, E. G., Bonilla, M., Liang, L., Takita, Y., Cunningham, K. W. (2000). A Homolog of Voltage-Gated Ca2+ Channels Stimulated by Depletion of Secretory Ca2+ in Yeast. Mol. Cell. Biol. 20: 6686-6694 [Abstract] [Full Text]  
  • Walberg, M. W. (2000). Applicability of Yeast Genetics to Neurologic Disease. Arch Neurol 57: 1129-1134 [Abstract] [Full Text]  
  • Jeziorski, M., Greenberg, R., Anderson, P. (2000). The molecular biology of invertebrate voltage-gated Ca(2+) channels. J. Exp. Biol. 203: 841-856 [Abstract]  
  • Gustin, M. C., Albertyn, J., Alexander, M., Davenport, K. (1998). MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264-1300 [Abstract] [Full Text]  
  • Stolz, L. E., Kuo, W. J., Longchamps, J., Sekhon, M. K., York, J. D. (1998). INP51, a Yeast Inositol Polyphosphate 5-Phosphatase Required for Phosphatidylinositol 4,5-Bisphosphate Homeostasis and Whose Absence Confers a Cold-resistant Phenotype. J. Biol. Chem. 273: 11852-11861 [Abstract] [Full Text]  
  • Forster, C., Kane, P. M. (2000). Cytosolic Ca2+ Homeostasis Is a Constitutive Function of the V-ATPase in Saccharomyces cerevisiae. J. Biol. Chem. 275: 38245-38253 [Abstract] [Full Text]  
  • Birchwood, C. J., Saba, J. D., Dickson, R. C., Cunningham, K. W. (2001). Calcium Influx and Signaling in Yeast Stimulated by Intracellular Sphingosine 1-Phosphate Accumulation. J. Biol. Chem. 276: 11712-11718 [Abstract] [Full Text]  
  • Palmer, C. P., Zhou, X.-L., Lin, J., Loukin, S. H., Kung, C., Saimi, Y. (2001). A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca2+-permeable channel in the yeast vacuolar membrane. Proc. Natl. Acad. Sci. USA 98: 7801-7805 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»