This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tantin, D. Articles by Carey, M. Search for Related Content PubMed PubMed Citation Articles by Tantin, D. Articles by Carey, M.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 12 1997, 6803-6814, Vol 17, No. 12
Copyright © 1997, American Society for Microbiology

Recruitment of the putative transcription-repair coupling factor CSB/ERCC6 to RNA polymerase II elongation complexes

D Tantin, A Kansal and M Carey
Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095-1737, USA.

Cockayne's syndrome (CS) is a disease characterized by developmental and growth defects, sunlight sensitivity, and a defect in transcription- coupled nucleotide excision repair. The two principle proteins involved in CS, CSA and CSB/ERCC6, have been hypothesized to bind RNA polymerase II (Pol II) and link transcription to DNA repair. We have tested CSA and CSB in assays designed to determine their role in transcription- coupled repair. Using a unique oligo(dC)-tailed DNA template, we provide biochemical evidence that CSB/ERCC6 interacts with Pol II molecules engaged in ternary complexes containing DNA and nascent RNA. CSB is a DNA-activated ATPase, and hydrolysis of the ATP beta-gamma phosphoanhydride bond is required for the formation of a stable Pol II- CSB-DNA-RNA complex. Unlike CSB, CSA does not directly bind Pol II.

This article has been cited by other articles:

• Escargueil, A. E., Poindessous, V., Soares, D. G., Sarasin, A., Cook, P. R., Larsen, A. K. (2008). Influence of irofulven, a transcription-coupled repair-specific antitumor agent, on RNA polymerase activity, stability and dynamics in living mammalian cells. J. Cell Sci. 121: 1275-1283 [Abstract] [Full Text]  
• Wong, H.-K., Muftuoglu, M., Beck, G., Imam, S. Z., Bohr, V. A., Wilson, D. M. III (2007). Cockayne syndrome B protein stimulates apurinic endonuclease 1 activity and protects against agents that introduce base excision repair intermediates. Nucleic Acids Res 0: gkm404v1-11 [Abstract] [Full Text]  
• Saijo, M., Hirai, T., Ogawa, A., Kobayashi, A., Kamiuchi, S., Tanaka, K. (2007). Functional TFIIH Is Required for UV-Induced Translocation of CSA to the Nuclear Matrix. Mol. Cell. Biol. 27: 2538-2547 [Abstract] [Full Text]  
• Newman, J. C., Bailey, A. D., Weiner, A. M. (2006). Cockayne syndrome group B protein (CSB) plays a general role in chromatin maintenance and remodeling. Proc. Natl. Acad. Sci. USA 103: 9613-9618 [Abstract] [Full Text]  
• Groisman, R., Kuraoka, I., Chevallier, O., Gaye, N., Magnaldo, T., Tanaka, K., Kisselev, A. F., Harel-Bellan, A., Nakatani, Y. (2006). CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome. Genes Dev. 20: 1429-1434 [Abstract] [Full Text]  
• Muftuoglu, M., Sharma, S., Thorslund, T., Stevnsner, T., Soerensen, M. M., Brosh, R. M. Jr, Bohr, V. A. (2006). Cockayne syndrome group B protein has novel strand annealing and exchange activities. Nucleic Acids Res 34: 295-304 [Abstract] [Full Text]  
• Gridasova, A. A., Henry, R. W. (2005). The p53 Tumor Suppressor Protein Represses Human snRNA Gene Transcription by RNA Polymerases II and III Independently of Sequence-Specific DNA Binding. Mol. Cell. Biol. 25: 3247-3260 [Abstract] [Full Text]  
• Beerens, N., Hoeijmakers, J. H. J., Kanaar, R., Vermeulen, W., Wyman, C. (2005). The CSB Protein Actively Wraps DNA. J. Biol. Chem. 280: 4722-4729 [Abstract] [Full Text]  
• Sims, R. J. III, Belotserkovskaya, R., Reinberg, D. (2004). Elongation by RNA polymerase II: the short and long of it. Genes Dev. 18: 2437-2468 [Abstract] [Full Text]  
• de Waard, H., de Wit, J., Andressoo, J.-O., van Oostrom, C. T. M., Riis, B., Weimann, A., Poulsen, H. E., van Steeg, H., Hoeijmakers, J. H. J., van der Horst, G. T. J. (2004). Different Effects of CSA and CSB Deficiency on Sensitivity to Oxidative DNA Damage. Mol. Cell. Biol. 24: 7941-7948 [Abstract] [Full Text]  
• van den Boom, V., Citterio, E., Hoogstraten, D., Zotter, A., Egly, J.-M., van Cappellen, W. A., Hoeijmakers, J. H.J., Houtsmuller, A. B., Vermeulen, W. (2004). DNA damage stabilizes interaction of CSB with the transcription elongation machinery. J. Cell Biol. 166: 27-36 [Abstract] [Full Text]  
• Peterson, C. L., Cote, J. (2004). Cellular machineries for chromosomal DNA repair. Genes Dev. 18: 602-616 [Full Text]  
• Tremeau-Bravard, A., Riedl, T., Egly, J.-M., Dahmus, M. E. (2004). Fate of RNA Polymerase II Stalled at a Cisplatin Lesion. J. Biol. Chem. 279: 7751-7759 [Abstract] [Full Text]  
• Krum, S. A., Miranda, G. A., Lin, C., Lane, T. F. (2003). BRCA1 Associates with Processive RNA Polymerase II. J. Biol. Chem. 278: 52012-52020 [Abstract] [Full Text]  
• Wu, X., Fan, W., Xu, S., Zhou, Y. (2003). Sensitization to the Cytotoxicity of Cisplatin by Transfection with Nucleotide Excision Repair Gene Xeroderma Pigmentosun Group A Antisense RNA in Human Lung Adenocarcinoma Cells. Clin. Cancer Res. 9: 5874-5879 [Abstract] [Full Text]  
• Pardee, T. S., Ghazy, M. A., Ponticelli, A. S. (2003). Yeast and Human RNA Polymerase II Elongation Complexes: Evidence for Functional Differences and Postinitiation Recruitment of Factors. Eukaryot Cell 2: 318-327 [Abstract] [Full Text]  
• Christiansen, M., Stevnsner, T., Modin, C., Martensen, P. M., Brosh, R. M. Jr, Bohr, V. A. (2003). Functional consequences of mutations in the conserved SF2 motifs and post-translational phosphorylation of the CSB protein. Nucleic Acids Res 31: 963-973 [Abstract] [Full Text]  
• Svejstrup, J. Q. (2003). Rescue of arrested RNA polymerase II complexes. J. Cell Sci. 116: 447-451 [Abstract] [Full Text]  
• Gonzalez-Barrera, S., Prado, F., Verhage, R., Brouwer, J., Aguilera, A. (2002). Defective nucleotide excision repair in yeast hpr1 and tho2 mutants. Nucleic Acids Res 30: 2193-2201 [Abstract] [Full Text]  
• Selzer, R. R., Nyaga, S., Tuo, J., May, A., Muftuoglu, M., Christiansen, M., Citterio, E., Brosh, R. M. Jr, Bohr, V. A. (2002). Differential requirement for the ATPase domain of the Cockayne syndrome group B gene in the processing of UV-induced DNA damage and 8-oxoguanine lesions in human cells. Nucleic Acids Res 30: 782-793 [Abstract] [Full Text]  
• Kamiuchi, S., Saijo, M., Citterio, E., de Jager, M., Hoeijmakers, J. H. J., Tanaka, K. (2002). Translocation of Cockayne syndrome group A protein to the nuclear matrix: Possible relevance to transcription-coupled DNA repair. Proc. Natl. Acad. Sci. USA 99: 201-206 [Abstract] [Full Text]  
• Murai, M., Enokido, Y., Inamura, N., Yoshino, M., Nakatsu, Y., van der Horst, G. T. J., Hoeijmakers, J. H. J., Tanaka, K., Hatanaka, H. (2001). Early postnatal ataxia and abnormal cerebellar development in mice lacking Xeroderma pigmentosum Group A and Cockayne Syndrome Group B DNA repair genes. Proc. Natl. Acad. Sci. USA 10.1073/pnas.231329598v1 [Abstract] [Full Text]  
• Lu, Y., Lian, H., Sharma, P., Schreiber-Agus, N., Russell, R. G., Chin, L., van der Horst, G. T. J., Bregman, D. B. (2001). Disruption of the Cockayne Syndrome B Gene Impairs Spontaneous Tumorigenesis in Cancer-Predisposed Ink4a/ARF Knockout Mice. Mol. Cell. Biol. 21: 1810-1818 [Abstract] [Full Text]  
• Citterio, E., Van Den Boom, V., Schnitzler, G., Kanaar, R., Bonte, E., Kingston, R. E., Hoeijmakers, J. H. J., Vermeulen, W. (2000). ATP-Dependent Chromatin Remodeling by the Cockayne Syndrome B DNA Repair-Transcription-Coupling Factor. Mol. Cell. Biol. 20: 7643-7653 [Abstract] [Full Text]  
• Sunesen, M., Selzer, R. R., Brosh Jr, R. M., Balajee, A. S., Stevnsner, T., Bohr, V. A. (2000). Molecular characterization of an acidic region deletion mutant of Cockayne syndrome group B protein. Nucleic Acids Res 28: 3151-3159 [Abstract] [Full Text]  
• Anderson, M. G., Scoggin, K. E. S., Simbulan-Rosenthal, C. M., Steadman, J. A. (2000). Identification of Poly(ADP-Ribose) Polymerase as a Transcriptional Coactivator of the Human T-Cell Leukemia Virus Type 1 Tax Protein. J. Virol. 74: 2169-2177 [Abstract] [Full Text]  
• Bochar, D. A., Savard, J., Wang, W., Lafleur, D. W., Moore, P., Cote, J., Shiekhattar, R. (2000). A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc. Natl. Acad. Sci. USA 97: 1038-1043 [Abstract] [Full Text]  
• Brosh, R. M. Jr., Balajee, A. S., Selzer, R. R., Sunesen, M., De Santis, L. P., Bohr, V. A. (1999). The ATPase Domain but Not the Acidic Region of Cockayne Syndrome Group B Gene Product Is Essential for DNA Repair. Mol. Biol. Cell 10: 3583-3594 [Abstract] [Full Text]  
• Jiang, H., Yang, L.-Y. (1999). Cell Cycle Checkpoint Abrogator UCN-01 Inhibits DNA Repair: Association with Attenuation of the Interaction of XPA and ERCC1 Nucleotide Excision Repair Proteins. Cancer Res. 59: 4529-4534 [Abstract] [Full Text]  
• Mitsui, A., Sharp, P. A. (1999). Ubiquitination of RNA polymerase II large subunit signaled by phosphorylation of carboxyl-terminal domain. Proc. Natl. Acad. Sci. USA 96: 6054-6059 [Abstract] [Full Text]  
• Martin, M. E. D., Berk, A. J. (1999). Corepressor Required for Adenovirus E1B 55,000-Molecular-Weight Protein Repression of Basal Transcription. Mol. Cell. Biol. 19: 3403-3414 [Abstract] [Full Text]  
• de Laat, W. L., Jaspers, N. G.J., Hoeijmakers, J. H.J. (1999). Molecular mechanism of nucleotide excision repair. Genes Dev. 13: 768-785 [Full Text]  
• Auble, D. T., Steggerda, S. M. (1999). Testing for DNA Tracking by MOT1, a SNF2/SWI2 Protein Family Member. Mol. Cell. Biol. 19: 412-423 [Abstract] [Full Text]  
• Woodard, R. L., Anderson, M. G., Dynan, W. S. (1999). Nuclear Extracts Lacking DNA-dependent Protein Kinase Are Deficient in Multiple Round Transcription. J. Biol. Chem. 274: 478-485 [Abstract] [Full Text]  
• Tantin, D. (1998). RNA Polymerase II Elongation Complexes Containing the Cockayne Syndrome Group B Protein Interact with a Molecular Complex Containing the Transcription Factor IIH Components Xeroderma Pigmentosum B and p62. J. Biol. Chem. 273: 27794-27799 [Abstract] [Full Text]  
• Kumar, K. P., Akoulitchev, S., Reinberg, D. (1998). Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event. Proc. Natl. Acad. Sci. USA 95: 9767-9772 [Abstract] [Full Text]  
• Citterio, E., Rademakers, S., van der Horst, G. T. J., van Gool, A. J., Hoeijmakers, J. H.J., Vermeulen, W. (1998). Biochemical and Biological Characterization of Wild-type and ATPase-deficient Cockayne Syndrome B Repair Protein. J. Biol. Chem. 273: 11844-11851 [Abstract] [Full Text]  
• You, Z., Feaver, W. J., Friedberg, E. C. (1998). Yeast RNA Polymerase II Transcription In Vitro Is Inhibited in the Presence of Nucleotide Excision Repair: Complementation of Inhibition by Holo-TFIIH and Requirement for RAD26. Mol. Cell. Biol. 18: 2668-2676 [Abstract] [Full Text]  
• Nakatsu, Y., Asahina, H., Citterio, E., Rademakers, S., Vermeulen, W., Kamiuchi, S., Yeo, J.-P., Khaw, M.-C., Saijo, M., Kodo, N., Matsuda, T., Hoeijmakers, J. H. J., Tanaka, K. (2000). XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription. J. Biol. Chem. 275: 34931-34937 [Abstract] [Full Text]  
• Murai, M., Enokido, Y., Inamura, N., Yoshino, M., Nakatsu, Y., van der Horst, G. T. J., Hoeijmakers, J. H. J., Tanaka, K., Hatanaka, H. (2001). Early postnatal ataxia and abnormal cerebellar development in mice lacking Xeroderma pigmentosum Group A and Cockayne Syndrome Group B DNA repair genes. Proc. Natl. Acad. Sci. USA 98: 13379-13384 [Abstract] [Full Text]