Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: a role for paxillin tyrosine phosphorylation

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Richardson, A.
	Articles by Parsons, J. T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Richardson, A.
	Articles by Parsons, J. T.

Previous Article | Next Article

Mol. Cell. Biol., Dec 1997, 6906-6914, Vol 17, No. 12
Copyright © 1997, American Society for Microbiology

Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: a role for paxillin tyrosine phosphorylation

A Richardson, RK Malik, JD Hildebrand and JT Parsons
Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville 22908, USA.

pp125FAK is a tyrosine kinase that appears to regulate the assembly of focal adhesions and thereby promotes cell spreading on the extracellular matrix. In some cells, the C terminus of pp125FAK is expressed as a separate protein, pp41/43FRNK. We have previously shown that overexpression of pp41/43FRNK inhibits tyrosine phosphorylation of pp125FAK and paxillin and, in addition, delays cell spreading and focal adhesion assembly. Thus, pp41/43FRNK functions as a negative inhibitor of adhesion signaling and provides a tool to dissect the mechanism by which pp125FAK promotes cell spreading. We report here that the inhibitory effects of pp41/43FRNK expression can be rescued by the co- overexpression of wild-type pp125FAK and partially rescued by catalytically inactive variants of pp125FAK. However, coexpression of an autophosphorylation site mutant of pp125FAK, which fails to bind the SH2 domain of pp60c-Src, or a mutant that fails to bind paxillin did not promote cell spreading. In contrast, expression of pp41/43FRNK and pp60c-Src reconstituted cell spreading and tyrosine phosphorylation of paxillin but did so without inducing tyrosine phosphorylation of pp125FAK. These data provide additional support for a model whereby pp125FAK acts as a "switchable adaptor" that recruits pp60c-Src to phosphorylate paxillin, promoting cell spreading. In addition, these data point to tyrosine phosphorylation of paxillin as being a critical step in focal adhesion assembly.

This article has been cited by other articles:

Antoku, S., Saksela, K., Rivera, G. M., Mayer, B. J. (2008). A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors. J. Cell Sci. 121: 3071-3082 [Abstract] [Full Text]
Shi, X., Qu, H., Kretzler, M., Wu, C. (2008). Roles of PINCH-2 in regulation of glomerular cell shape change and fibronectin matrix deposition. Am. J. Physiol. Renal Physiol. 295: F253-F263 [Abstract] [Full Text]
Parsons, J. T., Slack-Davis, J., Tilghman, R., Roberts, W. G. (2008). Focal Adhesion Kinase: Targeting Adhesion Signaling Pathways for Therapeutic Intervention. Clin. Cancer Res. 14: 627-632 [Abstract] [Full Text]
Srinivasan, R., Forman, S., Quinlan, R. A., Ohanian, J., Ohanian, V. (2008). Regulation of contractility by Hsp27 and Hic-5 in rat mesenteric small arteries. Am. J. Physiol. Heart Circ. Physiol. 294: H961-H969 [Abstract] [Full Text]
Witham, J., Valenti, M. R., De-Haven-Brandon, A. K., Vidot, S., Eccles, S. A., Kaye, S. B., Richardson, A. (2007). The Bcl-2/Bcl-XL Family Inhibitor ABT-737 Sensitizes Ovarian Cancer Cells to Carboplatin. Clin. Cancer Res. 13: 7191-7198 [Abstract] [Full Text]
Zaidel-Bar, R., Milo, R., Kam, Z., Geiger, B. (2007). A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions. J. Cell Sci. 120: 137-148 [Abstract] [Full Text]
Chang, F., Lemmon, C. A., Park, D., Romer, L. H. (2007). FAK Potentiates Rac1 Activation and Localization to Matrix Adhesion Sites: A Role for betaPIX. Mol. Biol. Cell 18: 253-264 [Abstract] [Full Text]
Le Boeuf, F., Houle, F., Sussman, M., Huot, J. (2006). Phosphorylation of Focal Adhesion Kinase (FAK) on Ser732 Is Induced by Rho-dependent Kinase and Is Essential for Proline-rich Tyrosine Kinase-2-mediated Phosphorylation of FAK on Tyr407 in Response to Vascular Endothelial Growth Factor. Mol. Biol. Cell 17: 3508-3520 [Abstract] [Full Text]
Pirone, D. M., Liu, W. F., Ruiz, S. A., Gao, L., Raghavan, S., Lemmon, C. A., Romer, L. H., Chen, C. S. (2006). An inhibitory role for FAK in regulating proliferation: a link between limited adhesion and RhoA-ROCK signaling. J. Cell Biol. 174: 277-288 [Abstract] [Full Text]
Sakamoto, Y., Ogita, H., Hirota, T., Kawakatsu, T., Fukuyama, T., Yasumi, M., Kanzaki, N., Ozaki, M., Takai, Y. (2006). Interaction of Integrin {alpha}vbeta3 with Nectin: IMPLICATION IN CROSS-TALK BETWEEN CELL-MATRIX AND CELL-CELL JUNCTIONS. J. Biol. Chem. 281: 19631-19644 [Abstract] [Full Text]
Kragtorp, K. A., Miller, J. R. (2006). Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development. Development 133: 685-695 [Abstract] [Full Text]
Robertson, L. K., Mireau, L. R., Ostergaard, H. L. (2005). A Role for Phosphatidylinositol 3-Kinase in TCR-Stimulated ERK Activation Leading to Paxillin Phosphorylation and CTL Degranulation. J. Immunol. 175: 8138-8145 [Abstract] [Full Text]
Usatyuk, P. V., Natarajan, V. (2005). Regulation of reactive oxygen species-induced endothelial cell-cell and cell-matrix contacts by focal adhesion kinase and adherens junction proteins. Am. J. Physiol. Lung Cell. Mol. Physiol. 289: L999-L1010 [Abstract] [Full Text]
Grigera, P. R., Jeffery, E. D., Martin, K. H., Shabanowitz, J., Hunt, D. F., Parsons, J. T. (2005). FAK phosphorylation sites mapped by mass spectrometry. J. Cell Sci. 118: 4931-4935 [Full Text]
Brown, M. C., Cary, L. A., Jamieson, J. S., Cooper, J. A., Turner, C. E. (2005). Src and FAK Kinases Cooperate to Phosphorylate Paxillin Kinase Linker, Stimulate Its Focal Adhesion Localization, and Regulate Cell Spreading and Protrusiveness. Mol. Biol. Cell 16: 4316-4328 [Abstract] [Full Text]
Moh, M. C., Zhang, C., Luo, C., Lee, L. H., Shen, S. (2005). Structural and Functional Analyses of a Novel Ig-like Cell Adhesion Molecule, hepaCAM, in the Human Breast Carcinoma MCF7 Cells. J. Biol. Chem. 280: 27366-27374 [Abstract] [Full Text]
Golubovskaya, V. M., Finch, R., Cance, W. G. (2005). Direct Interaction of the N-terminal Domain of Focal Adhesion Kinase with the N-terminal Transactivation Domain of p53. J. Biol. Chem. 280: 25008-25021 [Abstract] [Full Text]
Yang, Y., Guo, L., Blattner, S. M., Mundel, P., Kretzler, M., Wu, C. (2005). Formation and Phosphorylation of the PINCH-1-Integrin Linked Kinase-{alpha}-Parvin Complex Are Important for Regulation of Renal Glomerular Podocyte Adhesion, Architecture, and Survival. J. Am. Soc. Nephrol. 16: 1966-1976 [Abstract] [Full Text]
van Nimwegen, M. J., Verkoeijen, S., van Buren, L., Burg, D., van de Water, B. (2005). Requirement for Focal Adhesion Kinase in the Early Phase of Mammary Adenocarcinoma Lung Metastasis Formation. Cancer Res. 65: 4698-4706 [Abstract] [Full Text]
von Sengbusch, A., Gassmann, P., Fisch, K. M., Enns, A., Nicolson, G. L., Haier, J. (2005). Focal Adhesion Kinase Regulates Metastatic Adhesion of Carcinoma Cells within Liver Sinusoids. Am. J. Pathol. 166: 585-596 [Abstract] [Full Text]
Iwahara, T., Akagi, T., Fujitsuka, Y., Hanafusa, H. (2004). CrkII regulates focal adhesion kinase activation by making a complex with Crk-associated substrate, p130Cas. Proc. Natl. Acad. Sci. USA 101: 17693-17698 [Abstract] [Full Text]
De Seranno, S., Estrella, C., Loyens, A., Cornea, A., Ojeda, S. R., Beauvillain, J.-C., Prevot, V. (2004). Vascular Endothelial Cells Promote Acute Plasticity in Ependymoglial Cells of the Neuroendocrine Brain. J. Neurosci. 24: 10353-10363 [Abstract] [Full Text]
Avraham, H. K., Jiang, S., Lee, T.-H., Prakash, O., Avraham, S. (2004). HIV-1 Tat-Mediated Effects on Focal Adhesion Assembly and Permeability in Brain Microvascular Endothelial Cells. J. Immunol. 173: 6228-6233 [Abstract] [Full Text]
Lunn, J. A., Rozengurt, E. (2004). Hyperosmotic Stress Induces Rapid Focal Adhesion Kinase Phosphorylation at Tyrosines 397 and 577: ROLE OF Src FAMILY KINASES AND Rho FAMILY GTPases. J. Biol. Chem. 279: 45266-45278 [Abstract] [Full Text]
Hopkins, A. M., Bruewer, M., Brown, G. T., Pineda, A. A., Ha, J. J., Winfree, L. M., Walsh, S. V., Babbin, B. A., Nusrat, A. (2004). Epithelial cell spreading induced by hepatocyte growth factor influences paxillin protein synthesis and posttranslational modification. Am. J. Physiol. Gastrointest. Liver Physiol. 287: G886-G898 [Abstract] [Full Text]
Westhoff, M. A., Serrels, B., Fincham, V. J., Frame, M. C., Carragher, N. O. (2004). Src-Mediated Phosphorylation of Focal Adhesion Kinase Couples Actin and Adhesion Dynamics to Survival Signaling. Mol. Cell. Biol. 24: 8113-8133 [Abstract] [Full Text]
Mukhopadhyay, N. K., Gilchrist, D., Gordon, G. J., Chen, C.-J., Bueno, R., Lu, M. L., Salgia, R., Sugarbaker, D. J., Jaklitsch, M. T. (2004). Integrin dependent protein tyrosine phosphorylation is a key regulatory event in collagen IV mediated adhesion and proliferation of human lung tumor cell line, Calu-1. Ann. Thorac. Surg. 78: 450-457 [Abstract] [Full Text]
Romanova, L. Y., Hashimoto, S., Chay, K.-O., Blagosklonny, M. V., Sabe, H., Mushinski, J. F. (2004). Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J. Cell Sci. 117: 3759-3768 [Abstract] [Full Text]
Giannone, G., Ronde, P., Gaire, M., Beaudouin, J., Haiech, J., Ellenberg, J., Takeda, K. (2004). Calcium Rises Locally Trigger Focal Adhesion Disassembly and Enhance Residency of Focal Adhesion Kinase at Focal Adhesions. J. Biol. Chem. 279: 28715-28723 [Abstract] [Full Text]
Bian, D., Su, S., Mahanivong, C., Cheng, R. K., Han, Q., Pan, Z. K., Sun, P., Huang, S. (2004). Lysophosphatidic Acid Stimulates Ovarian Cancer Cell Migration via a Ras-MEK Kinase 1 Pathway. Cancer Res. 64: 4209-4217 [Abstract] [Full Text]
Armulik, A., Velling, T., Johansson, S. (2004). The Integrin {beta}1 Subunit Transmembrane Domain Regulates Phosphatidylinositol 3-Kinase-dependent Tyrosine Phosphorylation of Crk-associated Substrate. Mol. Biol. Cell 15: 2558-2567 [Abstract] [Full Text]
Fukuda, T., Chen, K., Shi, X., Wu, C. (2003). PINCH-1 Is an Obligate Partner of Integrin-linked Kinase (ILK) Functioning in Cell Shape Modulation, Motility, and Survival. J. Biol. Chem. 278: 51324-51333 [Abstract] [Full Text]
Zlot, C., Ingle, G., Hongo, J., Yang, S., Sheng, Z., Schwall, R., Paoni, N., Wang, F., Peale, F. V. Jr., Gerritsen, M. E. (2003). Stanniocalcin 1 Is an Autocrine Modulator of Endothelial Angiogenic Responses to Hepatocyte Growth Factor. J. Biol. Chem. 278: 47654-47659 [Abstract] [Full Text]
Avraham, H. K., Lee, T.-H., Koh, Y., Kim, T.-A., Jiang, S., Sussman, M., Samarel, A. M., Avraham, S. (2003). Vascular Endothelial Growth Factor Regulates Focal Adhesion Assembly in Human Brain Microvascular Endothelial Cells through Activation of the Focal Adhesion Kinase and Related Adhesion Focal Tyrosine Kinase. J. Biol. Chem. 278: 36661-36668 [Abstract] [Full Text]
Roovers, K., Assoian, R. K. (2003). Effects of Rho Kinase and Actin Stress Fibers on Sustained Extracellular Signal-Regulated Kinase Activity and Activation of G1 Phase Cyclin-Dependent Kinases. Mol. Cell. Biol. 23: 4283-4294 [Abstract] [Full Text]
Rose, D. M., Liu, S., Woodside, D. G., Han, J., Schlaepfer, D. D., Ginsberg, M. H. (2003). Paxillin Binding to the {alpha}4 Integrin Subunit Stimulates LFA-1 (Integrin {alpha}L{beta}2)-Dependent T Cell Migration by Augmenting the Activation of Focal Adhesion Kinase/Proline-Rich Tyrosine Kinase-2. J. Immunol. 170: 5912-5918 [Abstract] [Full Text]
Hunger-Glaser, I., Salazar, E. P., Sinnett-Smith, J., Rozengurt, E. (2003). Bombesin, Lysophosphatidic Acid, and Epidermal Growth Factor Rapidly Stimulate Focal Adhesion Kinase Phosphorylation at Ser-910: REQUIREMENT FOR ERK ACTIVATION. J. Biol. Chem. 278: 22631-22643 [Abstract] [Full Text]
Neumeister, P., Pixley, F. J., Xiong, Y., Xie, H., Wu, K., Ashton, A., Cammer, M., Chan, A., Symons, M., Stanley, E. R., Pestell, R. G. (2003). Cyclin D1 Governs Adhesion and Motility of Macrophages. Mol. Biol. Cell 14: 2005-2015 [Abstract] [Full Text]
Parsons, J. T. (2003). Focal adhesion kinase: the first ten years. J. Cell Sci. 116: 1409-1416 [Abstract] [Full Text]
Medley, Q. G., Buchbinder, E. G., Tachibana, K., Ngo, H., Serra-Pages, C., Streuli, M. (2003). Signaling between Focal Adhesion Kinase and Trio. J. Biol. Chem. 278: 13265-13270 [Abstract] [Full Text]
Reif, S., Lang, A., Lindquist, J. N., Yata, Y., Gabele, E., Scanga, A., Brenner, D. A., Rippe, R. A. (2003). The Role of Focal Adhesion Kinase-Phosphatidylinositol 3-Kinase-Akt Signaling in Hepatic Stellate Cell Proliferation and Type I Collagen Expression. J. Biol. Chem. 278: 8083-8090 [Abstract] [Full Text]
Chan, P.-C., Liang, C.-C., Yu, K.-C., Chang, M.-C., Ho, W. L., Chen, B.-H., Chen, H.-C. (2002). Synergistic Effect of Focal Adhesion Kinase Overexpression and Hepatocyte Growth Factor Stimulation on Cell Transformation. J. Biol. Chem. 277: 50373-50379 [Abstract] [Full Text]
Zhang, Y., Chen, K., Guo, L., Wu, C. (2002). Characterization of PINCH-2, a New Focal Adhesion Protein That Regulates the PINCH-1-ILK Interaction, Cell Spreading, and Migration. J. Biol. Chem. 277: 38328-38338 [Abstract] [Full Text]
Golubovskaya, V., Beviglia, L., Xu, L.-H., Earp, H. S. III, Craven, R., Cance, W. (2002). Dual Inhibition of Focal Adhesion Kinase and Epidermal Growth Factor Receptor Pathways Cooperatively Induces Death Receptor-mediated Apoptosis in Human Breast Cancer Cells. J. Biol. Chem. 277: 38978-38987 [Abstract] [Full Text]
Abbi, S., Ueda, H., Zheng, C., Cooper, L. A., Zhao, J., Christopher, R., Guan, J.-L. (2002). Regulation of Focal Adhesion Kinase by a Novel Protein Inhibitor FIP200. Mol. Biol. Cell 13: 3178-3191 [Abstract] [Full Text]
Giannone, G., Ronde, P., Gaire, M., Haiech, J., Takeda, K. (2002). Calcium Oscillations Trigger Focal Adhesion Disassembly in Human U87 Astrocytoma Cells. J. Biol. Chem. 277: 26364-26371 [Abstract] [Full Text]
Hermanto, U., Zong, C. S., Li, W., Wang, L.-H. (2002). RACK1, an Insulin-Like Growth Factor I (IGF-I) Receptor-Interacting Protein, Modulates IGF-I-Dependent Integrin Signaling and Promotes Cell Spreading and Contact with Extracellular Matrix. Mol. Cell. Biol. 22: 2345-2365 [Abstract] [Full Text]
Liu, Z.-X., Yu, C. F., Nickel, C., Thomas, S., Cantley, L. G. (2002). Hepatocyte Growth Factor Induces ERK-dependent Paxillin Phosphorylation and Regulates Paxillin-Focal Adhesion Kinase Association. J. Biol. Chem. 277: 10452-10458 [Abstract] [Full Text]
Li, L., Okura, M., Imamoto, A. (2002). Focal Adhesions Require Catalytic Activity of Src Family Kinases To Mediate Integrin-Matrix Adhesion. Mol. Cell. Biol. 22: 1203-1217 [Abstract] [Full Text]
Wang, Y.-H., Li, F., Schwartz, J. H., Flint, P. J., Borkan, S. C. (2001). c-Src and HSP72 interact in ATP-depleted renal epithelial cells. Am. J. Physiol. Cell Physiol. 281: C1667-C1675 [Abstract] [Full Text]
Hauck, C. R., Sieg, D. J., Hsia, D. A., Loftus, J. C., Gaarde, W. A., Monia, B. P., Schlaepfer, D. D. (2001). Inhibition of Focal Adhesion Kinase Expression or Activity Disrupts Epidermal Growth Factor-stimulated Signaling Promoting the Migration of Invasive Human Carcinoma Cells. Cancer Res. 61: 7079-7090 [Abstract] [Full Text]
Nishiya, N., Tachibana, K., Shibanuma, M., Mashimo, J.-i., Nose, K. (2001). Hic-5-Reduced Cell Spreading on Fibronectin: Competitive Effects between Paxillin and Hic-5 through Interaction with Focal Adhesion Kinase. Mol. Cell. Biol. 21: 5332-5345 [Abstract] [Full Text]
Gerthoffer, W. T., Gunst, S. J. (2001). Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle. J. Appl. Physiol. 91: 963-972 [Abstract] [Full Text]
Ray, R. M., Viar, M. J., McCormack, S. A., Johnson, L. R. (2001). Focal adhesion kinase signaling is decreased in polyamine-depleted IEC-6 cells. Am. J. Physiol. Cell Physiol. 281: C475-C485 [Abstract] [Full Text]
Laukaitis, C. M., Webb, D. J., Donais, K., Horwitz, A. F. (2001). Differential Dynamics of {alpha}5 Integrin, Paxillin, and {alpha}-Actinin during Formation and Disassembly of Adhesions in Migrating Cells. J. Cell Biol. 153: 1427-1440 [Abstract] [Full Text]
Lu, Z., Jiang, G., Blume-Jensen, P., Hunter, T. (2001). Epidermal Growth Factor-Induced Tumor Cell Invasion and Metastasis Initiated by Dephosphorylation and Downregulation of Focal Adhesion Kinase. Mol. Cell. Biol. 21: 4016-4031 [Abstract] [Full Text]
Tu, Y., Huang, Y., Zhang, Y., Hua, Y., Wu, C. (2001). A New Focal Adhesion Protein that Interacts with Integrin-linked Kinase and Regulates Cell Adhesion and Spreading. J. Cell Biol. 153: 585-598 [Abstract] [Full Text]
Tang, D. D., Gunst, S. J. (2001). Depletion of focal adhesion kinase by antisense depresses contractile activation of smooth muscle. Am. J. Physiol. Cell Physiol. 280: C874-C883 [Abstract] [Full Text]
Ren, X.-R., Du, Q.-S., Huang, Y.-Z., Ao, S.-Z., Mei, L., Xiong, W.-C. (2001). Regulation of CDC42 GTPase by Proline-rich Tyrosine Kinase 2 Interacting with PSGAP, a Novel Pleckstrin Homology and Src Homology 3 Domain Containing rhoGAP Protein. J. Cell Biol. 152: 971-984 [Abstract] [Full Text]
Taylor, J. M., Mack, C. P., Nolan, K., Regan, C. P., Owens, G. K., Parsons, J. T. (2001). Selective Expression of an Endogenous Inhibitor of FAK Regulates Proliferation and Migration of Vascular Smooth Muscle Cells. Mol. Cell. Biol. 21: 1565-1572 [Abstract] [Full Text]
Cooley, M. A., Broome, J. M., Ohngemach, C., Romer, L. H., Schaller, M. D. (2000). Paxillin Binding Is Not the Sole Determinant of Focal Adhesion Localization or Dominant-Negative Activity of Focal Adhesion Kinase/Focal Adhesion Kinase-related Nonkinase. Mol. Biol. Cell 11: 3247-3263 [Abstract] [Full Text]
Wennerberg, K., Armulik, A., Sakai, T., Karlsson, M., Fässler, R., Schaefer, E. M., Mosher, D. F., Johansson, S. (2000). The Cytoplasmic Tyrosines of Integrin Subunit beta 1 Are Involved in Focal Adhesion Kinase Activation. Mol. Cell. Biol. 20: 5758-5765 [Abstract] [Full Text]
Yano, H., Uchida, H., Iwasaki, T., Mukai, M., Akedo, H., Nakamura, K., Hashimoto, S., Sabe, H. (2000). Paxillin alpha and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation. Proc. Natl. Acad. Sci. USA 97: 9076-9081 [Abstract] [Full Text]
Mehta, D., Tang, D. D., Wu, M.-F., Atkinson, S., Gunst, S. J. (2000). Role of Rho in Ca2+-insensitive contraction and paxillin tyrosine phosphorylation in smooth muscle. Am. J. Physiol. Cell Physiol. 279: C308-C318 [Abstract] [Full Text]
Xiong, W., Macklem, M, Parsons, J. (2000). Expression and characterization of splice variants of PYK2, a focal adhesion kinase-related protein. J. Cell Sci. 111: 1981-1991 [Abstract]
Rousseau, S., Houle, F., Kotanides, H., Witte, L., Waltenberger, J., Landry, J., Huot, J. (2000). Vascular Endothelial Growth Factor (VEGF)-driven Actin-based Motility Is Mediated by VEGFR2 and Requires Concerted Activation of Stress-activated Protein Kinase 2 (SAPK2/p38) and Geldanamycin-sensitive Phosphorylation of Focal Adhesion Kinase. J. Biol. Chem. 275: 10661-10672 [Abstract] [Full Text]
Lai, J.-F., Kao, S.-C., Jiang, S.-T., Tang, M.-J., Chan, P.-C., Chen, H.-C. (2000). Involvement of Focal Adhesion Kinase in Hepatocyte Growth Factor-induced Scatter of Madin-Darby Canine Kidney Cells. J. Biol. Chem. 275: 7474-7480 [Abstract] [Full Text]
Qiao, H., Saulnier, R., Patryzkat, A., Rahimi, N., Raptis, L., Rossiter, J., Tremblay, E., Elliott, B. (2000). Cooperative Effect of Hepatocyte Growth Factor and Fibronectin in Anchorage-independent Survival of Mammary Carcinoma Cells: Requirement for Phosphatidylinositol 3-Kinase Activity. Cell Growth Differ. 11: 123-133 [Abstract] [Full Text]
Shen, Y., Lyons, P., Cooley, M., Davidson, D., Veillette, A., Salgia, R., Griffin, J. D., Schaller, M. D. (2000). The Noncatalytic Domain of Protein-tyrosine Phosphatase-PEST Targets Paxillin for Dephosphorylation in Vivo. J. Biol. Chem. 275: 1405-1413 [Abstract] [Full Text]
Ruest, P. J., Roy, S., Shi, E., Mernaugh, R. L., Hanks, S. K. (2000). Phosphospecific Antibodies Reveal Focal Adhesion Kinase Activation Loop Phosphorylation in Nascent and Mature Focal Adhesions and Requirement for the Autophosphorylation Site. Cell Growth Differ. 11: 41-48 [Abstract] [Full Text]
Thomas, J. W., Cooley, M. A., Broome, J. M., Salgia, R., Griffin, J. D., Lombardo, C. R., Schaller, M. D. (1999). The Role of Focal Adhesion Kinase Binding in the Regulation of Tyrosine Phosphorylation of Paxillin. J. Biol. Chem. 274: 36684-36692 [Abstract] [Full Text]
Renshaw, M. W., Price, L. S., Schwartz, M. A. (1999). Focal Adhesion Kinase Mediates the Integrin Signaling Requirement for Growth Factor Activation of MAP Kinase. J. Cell Biol. 147: 611-618 [Abstract] [Full Text]
Schaller, M. D., Hildebrand, J. D., Parsons, J. T. (1999). Complex Formation with Focal Adhesion Kinase: A Mechanism to Regulate Activity and Subcellular Localization of Src Kinases. Mol. Biol. Cell 10: 3489-3505 [Abstract] [Full Text]
Nolan, K., Lacoste, J., Parsons, J. T. (1999). Regulated Expression of Focal Adhesion Kinase-Related Nonkinase, the Autonomously Expressed C-Terminal Domain of Focal Adhesion Kinase. Mol. Cell. Biol. 19: 6120-6129 [Abstract] [Full Text]
Han, D. C., Guan, J.-L. (1999). Association of Focal Adhesion Kinase with Grb7 and Its Role in Cell Migration. J. Biol. Chem. 274: 24425-24430 [Abstract] [Full Text]
Zhang, X., Chattopadhyay, A., Ji, Q.-s., Owen, J. D., Ruest, P. J., Carpenter, G., Hanks, S. K. (1999). Focal adhesion kinase promotes phospholipase C-gamma 1 activity. Proc. Natl. Acad. Sci. USA 96: 9021-9026 [Abstract] [Full Text]
Shen, Y., Schaller, M. D. (1999). Focal Adhesion Targeting: The Critical Determinant of FAK Regulation and Substrate Phosphorylation. Mol. Biol. Cell 10: 2507-2518 [Abstract] [Full Text]
Owen, J. D., Ruest, P. J., Fry, D. W., Hanks, S. K. (1999). Induced Focal Adhesion Kinase (FAK) Expression in FAK-Null Cells Enhances Cell Spreading and Migration Requiring Both Auto- and Activation Loop Phosphorylation Sites and Inhibits Adhesion-Dependent Tyrosine Phosphorylation of Pyk2. Mol. Cell. Biol. 19: 4806-4818 [Abstract] [Full Text]
Turner, C. E., Brown, M. C., Perrotta, J. A., Riedy, M.C., Nikolopoulos, S. N., McDonald, A. R., Bagrodia, S., Thomas, S., Leventhal, P. S. (1999). Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF-GAP Protein: A Role in Cytoskeletal Remodeling. J. Cell Biol. 145: 851-863 [Abstract] [Full Text]
Oh, E.-S., Gu, H., Saxton, T. M., Timms, J. F., Hausdorff, S., Frevert, E. U., Kahn, B. B., Pawson, T., Neel, B. G., Thomas, S. M. (1999). Regulation of Early Events in Integrin Signaling by Protein Tyrosine Phosphatase SHP-2. Mol. Cell. Biol. 19: 3205-3215 [Abstract] [Full Text]
Li, X., Dy, R. C., Cance, W. G., Graves, L. M., Earp, H. S. (1999). Interactions between Two Cytoskeleton-associated Tyrosine Kinases: Calcium-dependent Tyrosine Kinase and Focal Adhesion Tyrosine Kinase. J. Biol. Chem. 274: 8917-8924 [Abstract] [Full Text]
Angers-Loustau, A., Cote, J.-F., Charest, A., Dowbenko, D., Spencer, S., Lasky, L. A., Tremblay, M. L. (1999). Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts. J. Cell Biol. 144: 1019-1031 [Abstract] [Full Text]
Yamakita, Y., Totsukawa, G., Yamashiro, S., Fry, D., Zhang, X., Hanks, S. K., Matsumura, F. (1999). Dissociation of FAK/p130CAS/c-Src Complex during Mitosis: Role of Mitosis-specific Serine Phosphorylation of FAK. J. Cell Biol. 144: 315-324 [Abstract] [Full Text]
Takayama, Y., Tanaka, S., Nagai, K., Okada, M. (1999). Adenovirus-mediated Overexpression of C-terminal Src Kinase (Csk) in Type I Astrocytes Interferes with Cell Spreading and Attachment to Fibronectin. CORRELATION WITH TYROSINE PHOSPHORYLATIONS OF PAXILLIN AND FAK. J. Biol. Chem. 274: 2291-2297 [Abstract] [Full Text]
Sieg, D., Hauck, C., Schlaepfer, D. (1999). Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. J. Cell Sci. 112: 2677-2691 [Abstract]
Rahimi, N., Hung, W., Tremblay, E., Saulnier, R., Elliott, B. (1998). c-Src Kinase Activity Is Required for Hepatocyte Growth Factor-induced Motility and Anchorage-independent Growth of Mammary Carcinoma Cells. J. Biol. Chem. 273: 33714-33721 [Abstract] [Full Text]
Alrutz, M. A., Isberg, R. R. (1998). Involvement of focal adhesion kinase in invasin-mediated uptake. Proc. Natl. Acad. Sci. USA 95: 13658-13663 [Abstract] [Full Text]
Gervais, F. G., Thornberry, N. A., Ruffolo, S. C., Nicholson, D. W., Roy, S. (1998). Caspases Cleave Focal Adhesion Kinase during Apoptosis to Generate a FRNK-like Polypeptide. J. Biol. Chem. 273: 17102-17108 [Abstract] [Full Text]
Needham, L. K., Rozengurt, E. (1998). Galpha 12 and Galpha 13 Stimulate Rho-dependent Tyrosine Phosphorylation of Focal Adhesion Kinase, Paxillin, and p130 Crk-associated Substrate. J. Biol. Chem. 273: 14626-14632 [Abstract] [Full Text]
Nakamura, K., Yano, H., Uchida, H., Hashimoto, S., Schaefer, E., Sabe, H. (2000). Tyrosine Phosphorylation of Paxillin alpha Is Involved in Temporospatial Regulation of Paxillin-containing Focal Adhesion Formation and F-actin Organization in Motile Cells. J. Biol. Chem. 275: 27155-27164 [Abstract] [Full Text]
Escalante, M., Courtney, J., Chin, W. G., Teng, K. K., Kim, J.-I., Fajardo, J. E., Mayer, B. J., Hempstead, B. L., Birge, R. B. (2000). Phosphorylation of c-Crk II on the Negative Regulatory Tyr222 Mediates Nerve Growth Factor-induced Cell Spreading and Morphogenesis. J. Biol. Chem. 275: 24787-24797 [Abstract] [Full Text]
Hauck, C. R., Hsia, D. A., Schlaepfer, D. D. (2000). Focal Adhesion Kinase Facilitates Platelet-derived Growth Factor-BB-stimulated ERK2 Activation Required for Chemotaxis Migration of Vascular Smooth Muscle Cells. J. Biol. Chem. 275: 41092-41099 [Abstract] [Full Text]
Hashimoto, S., Tsubouchi, A., Mazaki, Y., Sabe, H. (2001). Interaction of Paxillin with p21-activated Kinase (PAK). ASSOCIATION OF PAXILLIN alpha WITH THE KINASE-INACTIVE AND THE Cdc42-ACTIVATED FORMS OF PAK3. J. Biol. Chem. 276: 6037-6045 [Abstract] [Full Text]
Laferriere, J., Houle, F., Taher, M. M., Valerie, K., Huot, J. (2001). Transendothelial Migration of Colon Carcinoma Cells Requires Expression of E-selectin by Endothelial Cells and Activation of Stress-activated Protein Kinase-2 (SAPK2/p38) in the Tumor Cells. J. Biol. Chem. 276: 33762-33772 [Abstract] [Full Text]
Lyons, P. D., Dunty, J. M., Schaefer, E. M., Schaller, M. D. (2001). Inhibition of the Catalytic Activity of Cell Adhesion Kinase beta by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation. J. Biol. Chem. 276: 24422-24431 [Abstract] [Full Text]
Salazar, E. P., Rozengurt, E. (2001). Src Family Kinases Are Required for Integrin-mediated but Not for G Protein-coupled Receptor Stimulation of Focal Adhesion Kinase Autophosphorylation at Tyr-397. J. Biol. Chem. 276: 17788-17795 [Abstract] [Full Text]
Wu, X., Davis, G. E., Meininger, G. A., Wilson, E., Davis, M. J. (2001). Regulation of the L-type Calcium Channel by alpha 5beta 1 Integrin Requires Signaling between Focal Adhesion Proteins. J. Biol. Chem. 276: 30285-30292 [Abstract] [Full Text]
Zhang, Y., Guo, L., Chen, K., Wu, C. (2002). A Critical Role of the PINCH-Integrin-linked Kinase Interaction in the Regulation of Cell Shape Change and Migration. J. Biol. Chem. 277: 318-326 [Abstract] [Full Text]
Taylor, J. M., Rovin, J. D., Parsons, J. T. (2000). A Role for Focal Adhesion Kinase in Phenylephrine-induced Hypertrophy of Rat Ventricular Cardiomyocytes. J. Biol. Chem. 275: 19250-19257 [Abstract] [Full Text]
Xu, L.-H., Yang, X., Bradham, C. A., Brenner, D. A., Baldwin, A. S. Jr., Craven, R. J., Cance, W. G. (2000). The Focal Adhesion Kinase Suppresses Transformation-associated, Anchorage-independent Apoptosis in Human Breast Cancer Cells. INVOLVEMENT OF DEATH RECEPTOR-RELATED SIGNALING PATHWAYS. J. Biol. Chem. 275: 30597-30604 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals