This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zou, L.
Right arrow Articles by Stillman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zou, L.
Right arrow Articles by Stillman, B.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 02 1997, 553-563, Vol 17, No. 2
Copyright © 1997, American Society for Microbiology

CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication

L Zou, J Mitchell and B Stillman
Cold Spring Harbor Laboratory, New York 11724, USA.

The CDC45 gene of Saccharomyces cerevisiae was isolated by complementation of the cold-sensitive cdc45-1 mutant and shown to be essential for cell viability. Although CDC45 genetically interacts with a group of MCM genes (CDC46, CDC47, and CDC54), the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member. Further genetic characterization of the cdc45-1 mutant demonstrated that it is synthetically lethal with orc2- 1, mcm2-1, and mcm3-1. These results not only reveal a functional connection between the origin recognition complex (ORC) and Cdc45p but also extend the CDC45-MCM genetic interaction to all known MCM family members that were shown to be involved in replication initiation. Initiation of DNA replication in cdc45-1 cells was defective, causing a delayed entry into S phase at the nonpermissive temperature, as well as a high plasmid loss rate which could be suppressed by tandem copies of replication origins. Furthermore, two-dimensional gels directly showed that chromosomal origins fired less frequently in cdc45-1 cells at the nonpermissive temperature. These findings suggest that Cdc45p, ORC, and Mcm proteins act in concert for replication initiation throughout the genome.


This article has been cited by other articles:

  • Ballabeni, A., Zamponi, R., Caprara, G., Melixetian, M., Bossi, S., Masiero, L., Helin, K. (2009). Human CDT1 Associates with CDC7 and Recruits CDC45 to Chromatin during S Phase. J. Biol. Chem. 284: 3028-3036 [Abstract] [Full Text]  
  • Masai, H., Taniyama, C., Ogino, K., Matsui, E., Kakusho, N., Matsumoto, S., Kim, J.-M., Ishii, A., Tanaka, T., Kobayashi, T., Tamai, K., Ohtani, K., Arai, K.-i. (2006). Phosphorylation of MCM4 by Cdc7 Kinase Facilitates Its Interaction with Cdc45 on the Chromatin. J. Biol. Chem. 281: 39249-39261 [Abstract] [Full Text]  
  • Yu, L., Castillo, L. P., Mnaimneh, S., Hughes, T. R., Brown, G. W. (2006). A Survey of Essential Gene Function in the Yeast Cell Division Cycle. Mol. Biol. Cell 17: 4736-4747 [Abstract] [Full Text]  
  • Moyer, S. E., Lewis, P. W., Botchan, M. R. (2006). Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proc. Natl. Acad. Sci. USA 103: 10236-10241 [Abstract] [Full Text]  
  • You, Z., Masai, H. (2005). DNA binding and helicase actions of mouse MCM4/6/7 helicase. Nucleic Acids Res 33: 3033-3047 [Abstract] [Full Text]  
  • Shechter, D., Ying, C. Y., Gautier, J. (2004). DNA Unwinding Is an MCM Complex-dependent and ATP Hydrolysis-dependent Process. J. Biol. Chem. 279: 45586-45593 [Abstract] [Full Text]  
  • Uchiyama, M., Wang, T. S.-F. (2004). The B-Subunit of DNA Polymerase {alpha}-Primase Associates with the Origin Recognition Complex for Initiation of DNA Replication. Mol. Cell. Biol. 24: 7419-7434 [Abstract] [Full Text]  
  • Wang, X., Ira, G., Tercero, J. A., Holmes, A. M., Diffley, J. F. X., Haber, J. E. (2004). Role of DNA Replication Proteins in Double-Strand Break-Induced Recombination in Saccharomyces cerevisiae. Mol. Cell. Biol. 24: 6891-6899 [Abstract] [Full Text]  
  • Cortez, D., Glick, G., Elledge, S. J. (2004). From The Cover: Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc. Natl. Acad. Sci. USA 101: 10078-10083 [Abstract] [Full Text]  
  • Suter, B., Tong, A., Chang, M., Yu, L., Brown, G. W., Boone, C., Rine, J. (2004). The Origin Recognition Complex Links Replication, Sister Chromatid Cohesion and Transcriptional Silencing in Saccharomyces cerevisiae. Genetics 167: 579-591 [Abstract] [Full Text]  
  • Fiorani, P., Reid, R. J. D., Schepis, A., Jacquiau, H. R., Guo, H., Thimmaiah, P., Benedetti, P., Bjornsti, M.-A. (2004). The Deubiquitinating Enzyme Doa4p Protects Cells from DNA Topoisomerase I Poisons. J. Biol. Chem. 279: 21271-21281 [Abstract] [Full Text]  
  • Forsburg, S. L. (2004). Eukaryotic MCM Proteins: Beyond Replication Initiation. Microbiol. Mol. Biol. Rev. 68: 109-131 [Abstract] [Full Text]  
  • Stevens, R., Grelon, M., Vezon, D., Oh, J., Meyer, P., Perennes, C., Domenichini, S., Bergounioux, C. (2004). A CDC45 Homolog in Arabidopsis Is Essential for Meiosis, as Shown by RNA Interference-Induced Gene Silencing. Plant Cell 16: 99-113 [Abstract] [Full Text]  
  • Feng, D., Tu, Z., Wu, W., Liang, C. (2003). Inhibiting the Expression of DNA Replication-Initiation Proteins Induces Apoptosis in Human Cancer Cells. Cancer Res. 63: 7356-7364 [Abstract] [Full Text]  
  • Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., Araki, H. (2003). GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev. 17: 1153-1165 [Abstract] [Full Text]  
  • Chou, D. M., Petersen, P., Walter, J. C., Walter, G. (2002). Protein Phosphatase 2A Regulates Binding of Cdc45 to the Prereplication Complex. J. Biol. Chem. 277: 40520-40527 [Abstract] [Full Text]  
  • Xu, H., Wu, P., Wu, C. F. J., Tidwell, C., Wang, Y. (2002). A smooth response surface algorithm for constructing a gene regulatory network. Physiol. Genomics 11: 11-20 [Abstract] [Full Text]  
  • Nakajima, R., Masukata, H. (2002). SpSld3 Is Required for Loading and Maintenance of SpCdc45 on Chromatin in DNA Replication in Fission Yeast. Mol. Biol. Cell 13: 1462-1472 [Abstract] [Full Text]  
  • Zhu, W., Rainville, I. R., Ding, M., Bolus, M., Heintz, N. H., Pederson, D. S. (2002). Evidence That the pre-mRNA Splicing Factor Clf1p Plays a Role in DNA Replication in Saccharomyces cerevisiae. Genetics 160: 1319-1333 [Abstract] [Full Text]  
  • Jacobson, M. D., Munoz, C. X., Knox, K. S., Williams, B. E., Lu, L. L., Cross, F. R., Vallen, E. A. (2001). Mutations in SID2, a Novel Gene in Saccharomyces cerevisiae, Cause Synthetic Lethality With sic1 Deletion and May Cause a Defect During S Phase. Genetics 159: 17-33 [Abstract] [Full Text]  
  • Yoshida, K., Kuo, F., George, E. L., Sharpe, A. H., Dutta, A. (2001). Requirement of CDC45 for Postimplantation Mouse Development. Mol. Cell. Biol. 21: 4598-4603 [Abstract] [Full Text]  
  • Loebel, D., Huikeshoven, H., Cotterill, S. (2000). Localisation of the DmCdc45 DNA replication factor in the mitotic cycle and during chorion gene amplification. Nucleic Acids Res 28: 3897-3903 [Abstract] [Full Text]  
  • Jares, P., Blow, J. J. (2000). Xenopus Cdc7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading. Genes Dev. 14: 1528-1540 [Abstract] [Full Text]  
  • Nougarède, R., Della Seta, F., Zarzov, P., Schwob, E. (2000). Hierarchy of S-Phase-Promoting Factors: Yeast Dbf4-Cdc7 Kinase Requires Prior S-Phase Cyclin-Dependent Kinase Activation. Mol. Cell. Biol. 20: 3795-3806 [Abstract] [Full Text]  
  • Zou, L., Stillman, B. (2000). Assembly of a Complex Containing Cdc45p, Replication Protein A, and Mcm2p at Replication Origins Controlled by S-Phase Cyclin-Dependent Kinases and Cdc7p-Dbf4p Kinase. Mol. Cell. Biol. 20: 3086-3096 [Abstract] [Full Text]  
  • Masumoto, H., Sugino, A., Araki, H. (2000). Dpb11 Controls the Association between DNA Polymerases alpha and varepsilon and the Autonomously Replicating Sequence Region of Budding Yeast. Mol. Cell. Biol. 20: 2809-2817 [Abstract] [Full Text]  
  • Homesley, L., Lei, M., Kawasaki, Y., Sawyer, S., Christensen, T., Tye, B. K. (2000). Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev. 14: 913-926 [Abstract] [Full Text]  
  • Arata, Y., Fujita, M., Ohtani, K., Kijima, S., Kato, J.-y. (2000). Cdk2-dependent and -independent Pathways in E2F-mediated S Phase Induction. J. Biol. Chem. 275: 6337-6345 [Abstract] [Full Text]  
  • DIFFLEY, J.F.X., BOUSSET, K., LABIB, K., NOTON, E.A., SANTOCANALE, C., TERCERO, J.A. (2000). Coping with and Recovering from Hydroxyurea-induced Replication Fork Arrest in Budding Yeast. Cold Spring Harb Symp Quant Biol 65: 333-342 [Abstract]  
  • You, Z., Komamura, Y., Ishimi, Y. (1999). Biochemical Analysis of the Intrinsic Mcm4-Mcm6-Mcm7 DNA Helicase Activity. Mol. Cell. Biol. 19: 8003-8015 [Abstract] [Full Text]  
  • Ehrenhofer-Murray, A. E., Kamakaka, R. T., Rine, J. (1999). A Role for the Replication Proteins PCNA, RF-C, Polymerase {epsilon} and Cdc45 in Transcriptional Silencing in Saccharomyces cerevisiae. Genetics 153: 1171-1182 [Abstract] [Full Text]  
  • Reid, R. J. D., Fiorani, P., Sugawara, M., Bjornsti, M.-A. (1999). CDC45 and DPB11 are required for processive DNA replication and resistance to DNA topoisomerase I-mediated DNA damage. Proc. Natl. Acad. Sci. USA 96: 11440-11445 [Abstract] [Full Text]  
  • Oshiro, G., Owens, J. C., Shellman, Y., Sclafani, R. A., Li, J. J. (1999). Cell Cycle Control of Cdc7p Kinase Activity through Regulation of Dbf4p Stability. Mol. Cell. Biol. 19: 4888-4896 [Abstract] [Full Text]  
  • Snaith, H. A., Forsburg, S. L. (1999). Rereplication Phenomenon in Fission Yeast Requires MCM Proteins and Other S Phase Genes. Genetics 152: 839-851 [Abstract] [Full Text]  
  • Trowbridge, P. W., Roy, R., Simmons, D. T. (1999). Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro. Mol. Cell. Biol. 19: 1686-1694 [Abstract] [Full Text]  
  • Sanchez, M, Calzada, A, Bueno, A (1999). Functionally homologous DNA replication genes in fission and budding yeast. J. Cell Sci. 112: 2381-2390 [Abstract]  
  • Kamimura, Y., Masumoto, H., Sugino, A., Araki, H. (1998). Sld2, Which Interacts with Dpb11 in Saccharomyces cerevisiae, Is Required for Chromosomal DNA Replication. Mol. Cell. Biol. 18: 6102-6109 [Abstract] [Full Text]  
  • Ritzi, M., Baack, M., Musahl, C., Romanowski, P., Laskey, R. A., Knippers, R. (1998). Human Minichromosome Maintenance Proteins and Human Origin Recognition Complex 2 Protein on Chromatin. J. Biol. Chem. 273: 24543-24549 [Abstract] [Full Text]  
  • McKie, J. M., Wadey, R. B., Sutherland, H. F., Taylor, C. L., Scambler, P. J. (1998). Direct Selection of Conserved cDNAs from the DiGeorge Critical Region: Isolation of a Novel CDC45-Like Gene. Genome Res 8: 834-841 [Abstract] [Full Text]  
  • Saha, P., Thome, K. C., Yamaguchi, R., Hou, Z.-h., Weremowicz, S., Dutta, A. (1998). The Human Homolog of Saccharomyces cerevisiae CDC45. J. Biol. Chem. 273: 18205-18209 [Abstract] [Full Text]  
  • Caponigro, G., Abedi, M. R., Hurlburt, A. P., Maxfield, A., Judd, W., Kamb, A. (1998). Transdominant genetic analysis of a growth control pathway. Proc. Natl. Acad. Sci. USA 95: 7508-7513 [Abstract] [Full Text]  
  • Zou, L., Stillman, B. (1998). Formation of a Preinitiation Complex by S-phase Cyclin CDK-Dependent Loading of Cdc45p onto Chromatin. Science 280: 593-596 [Abstract] [Full Text]  
  • Holthoff, H. P., Baack, M., Richter, A., Ritzi, M., Knippers, R. (1998). Human Protein MCM6 on HeLa Cell Chromatin. J. Biol. Chem. 273: 7320-7325 [Abstract] [Full Text]  
  • Owens, J. C., Detweiler, C. S., Li, J. J. (1997). CDC45 is required in conjunction with CDC7/DBF4 to trigger the initiation of DNA replication. Proc. Natl. Acad. Sci. USA 94: 12521-12526 [Abstract] [Full Text]  
  • Lee, J.-K., Hurwitz, J. (2000). Isolation and Characterization of Various Complexes of the Minichromosome Maintenance Proteins of Schizosaccharomyces pombe. J. Biol. Chem. 275: 18871-18878 [Abstract] [Full Text]  
  • Chong, J. P. J., Hayashi, M. K., Simon, M. N., Xu, R.-M., Stillman, B. (2000). A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase. Proc. Natl. Acad. Sci. USA 97: 1530-1535 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»