This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Measday, V.
Right arrow Articles by Andrews, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Measday, V.
Right arrow Articles by Andrews, B.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 03 1997, 1212-1223, Vol 17, No. 3
Copyright © 1997, American Society for Microbiology

A family of cyclin-like proteins that interact with the Pho85 cyclin- dependent kinase

V Measday, L Moore, R Retnakaran, J Lee, M Donoviel, AM Neiman and B Andrews
Department of Molecular and Medical Genetics, University of Toronto, Canada.

In budding yeast, entry into the mitotic cell cycle, or Start, requires the Cdc28 cyclin-dependent kinase (Cdk) and one of its three associated G1 cyclins, Cln1, Cln2, or Cln3. In addition, two other G1 cyclins, Pcl1 and Pcl2, associate with a second Cdk, Pho85, to contribute to Start. Although Pho85 is not essential for viability, Pcl1,2-Pho85 kinase complexes become essential for Start in the absence of Cln1,2- Cdc28 kinases. In addition, Pho85 interacts with a third cyclin, Pho80, to regulate acid phosphatase gene expression. Other cellular roles for Pho85 cyclin-Cdk complexes are suggested by the multiple phenotypes associated with deletion of PHO85, in addition to Start defects and deregulated acid phosphatase gene expression. Strains with pho80, pcl1, and pcl2 deletions show only a subset of the pho85 mutant phenotypes, suggesting the existence of additional Pho85 cyclins (Pcls). We used two-hybrid screening and database searching to identify seven additional cyclin-related genes that may interact with Pho85. We found that all of the new genes encode proteins that interacted with Pho85 in an affinity chromatography assay. One of these genes, CLG1, was previously suggested to encode a cyclin, based on the protein's sequence homology to Pcl1 and Pcl2. We have named the other genes PCL5, PCL6, PCL7, PCL8, PCL9, and PCL10. On the basis of sequence similarities, the PCLs can be divided into two subfamilies: the Pcl1,2- like subfamily and the Pho80-like subfamily. We found that deletion of members of the Pcl1,2 class of genes resulted in pronounced morphological abnormalities. In addition, we found that expression of one member of the Pcl1,2 subfamily, PCL9, is cell cycle regulated and is decreased in cells arrested in G1 by pheromone treatment. Our studies suggest that Pho85 associates with multiple cyclins and that subsets of cyclins may direct Pho85 to perform distinct roles in cell growth and division.


This article has been cited by other articles:

  • Zou, J., Friesen, H., Larson, J., Huang, D., Cox, M., Tatchell, K., Andrews, B. (2009). Regulation of Cell Polarity through Phosphorylation of Bni4 by Pho85 G1 Cyclin-dependent Kinases in Saccharomyces cerevisiae. Mol. Biol. Cell 20: 3239-3250 [Abstract] [Full Text]  
  • Streckfuss-Bomeke, K., Schulze, F., Herzog, B., Scholz, E., Braus, G. H. (2009). Degradation of Saccharomyces cerevisiae Transcription Factor Gcn4 Requires a C-Terminal Nuclear Localization Signal in the Cyclin Pcl5. Eukaryot Cell 8: 496-510 [Abstract] [Full Text]  
  • Aviram, S., Simon, E., Gildor, T., Glaser, F., Kornitzer, D. (2008). Autophosphorylation-Induced Degradation of the Pho85 Cyclin Pcl5 Is Essential for Response to Amino Acid Limitation. Mol. Cell. Biol. 28: 6858-6869 [Abstract] [Full Text]  
  • Haynes, J., Garcia, B., Stollar, E. J., Rath, A., Andrews, B. J., Davidson, A. R. (2007). The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context. Genetics 176: 193-208 [Abstract] [Full Text]  
  • Rubenstein, E. M., Schmidt, M. C. (2007). Mechanisms Regulating the Protein Kinases of Saccharomyces cerevisiae. Eukaryot Cell 6: 571-583 [Full Text]  
  • Horiuchi, Y., Asada, A., Hisanaga, S.-i., Toh-e, A., Nishizawa, M. (2006). Identifying novel substrates for mouse Cdk5 kinase using the yeast Saccharomyces cerevisiae. GENES CELLS 11: 1393-1404 [Abstract] [Full Text]  
  • Gandhi, M., Goode, B. L., Chan, C. S. M. (2006). Four Novel Suppressors of gic1 gic2 and Their Roles in Cytokinesis and Polarized Cell Growth in Saccharomyces cerevisiae. Genetics 174: 665-678 [Abstract] [Full Text]  
  • Coic, E., Sun, K., Wu, C., Haber, J. E. (2006). Cell Cycle-Dependent Regulation of Saccharomyces cerevisiae Donor Preference during Mating-Type Switching by SBF (Swi4/Swi6) and Fkh1.. Mol. Cell. Biol. 26: 5470-5480 [Abstract] [Full Text]  
  • Bomeke, K., Pries, R., Korte, V., Scholz, E., Herzog, B., Schulze, F., Braus, G. H. (2006). Yeast Gcn4p Stabilization Is Initiated by the Dissociation of the Nuclear Pho85p/Pcl5p Complex. Mol. Biol. Cell 17: 2952-2962 [Abstract] [Full Text]  
  • Ren, G., Vajjhala, P., Lee, J. S., Winsor, B., Munn, A. L. (2006). The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy. Microbiol. Mol. Biol. Rev. 70: 37-120 [Abstract] [Full Text]  
  • Friesen, H., Colwill, K., Robertson, K., Schub, O., Andrews, B. (2005). Interaction of the Saccharomyces cerevisiae Cortical Actin Patch Protein Rvs167p With Proteins Involved in ER to Golgi Vesicle Trafficking. Genetics 170: 555-568 [Abstract] [Full Text]  
  • Iwaki, S., Kihara, A., Sano, T., Igarashi, Y. (2005). Phosphorylation by Pho85 Cyclin-dependent Kinase Acts as a Signal for the Down-regulation of the Yeast Sphingoid Long-chain Base Kinase Lcb4 during the Stationary Phase. J. Biol. Chem. 280: 6520-6527 [Abstract] [Full Text]  
  • Gildor, T., Shemer, R., Atir-Lande, A., Kornitzer, D. (2005). Coevolution of Cyclin Pcl5 and Its Substrate Gcn4. Eukaryot Cell 4: 310-318 [Abstract] [Full Text]  
  • Wu, D., Dou, X., Hashmi, S. B., Osmani, S. A. (2004). The Pho80-like Cyclin of Aspergillus nidulans Regulates Development Independently of Its Role in Phosphate Acquisition. J. Biol. Chem. 279: 37693-37703 [Abstract] [Full Text]  
  • Hammarton, T. C., Engstler, M., Mottram, J. C. (2004). The Trypanosoma brucei Cyclin, CYC2, Is Required for Cell Cycle Progression through G1 Phase and for Maintenance of Procyclic Form Cell Morphology. J. Biol. Chem. 279: 24757-24764 [Abstract] [Full Text]  
  • Willis, K. A., Barbara, K. E., Menon, B. B., Moffat, J., Andrews, B., Santangelo, G. M. (2003). The Global Transcriptional Activator of Saccharomyces cerevisiae, Gcr1p, Mediates the Response to Glucose by Stimulating Protein Synthesis and CLN-Dependent Cell Cycle Progression. Genetics 165: 1017-1029 [Abstract] [Full Text]  
  • Dou, X., Wu, D., An, W., Davies, J., Hashmi, S. B., Ukil, L., Osmani, S. A. (2003). The PHOA and PHOB Cyclin-Dependent Kinases Perform an Essential Function in Aspergillus nidulans. Genetics 165: 1105-1115 [Abstract] [Full Text]  
  • Costanzo, M., Schub, O., Andrews, B. (2003). G1 Transcription Factors Are Differentially Regulated in Saccharomyces cerevisiae by the Swi6-Binding Protein Stb1. Mol. Cell. Biol. 23: 5064-5077 [Abstract] [Full Text]  
  • Friesen, H., Murphy, K., Breitkreutz, A., Tyers, M., Andrews, B. (2003). Regulation of the Yeast Amphiphysin Homologue Rvs167p by Phosphorylation. Mol. Biol. Cell 14: 3027-3040 [Abstract] [Full Text]  
  • Tan, Y. S. H., Morcos, P. A., Cannon, J. F. (2003). Pho85 Phosphorylates the Glc7 Protein Phosphatase Regulator Glc8 in Vivo. J. Biol. Chem. 278: 147-153 [Abstract] [Full Text]  
  • Stadler, J. A., Schweyen, R. J. (2002). The Yeast Iron Regulon Is Induced upon Cobalt Stress and Crucial for Cobalt Tolerance. J. Biol. Chem. 277: 39649-39654 [Abstract] [Full Text]  
  • Shemer, R., Meimoun, A., Holtzman, T., Kornitzer, D. (2002). Regulation of the Transcription Factor Gcn4 by Pho85 Cyclin Pcl5. Mol. Cell. Biol. 22: 5395-5404 [Abstract] [Full Text]  
  • Huang, D., Moffat, J., Andrews, B. (2002). Dissection of a Complex Phenotype by Functional Genomics Reveals Roles for the Yeast Cyclin-Dependent Protein Kinase Pho85 in Stress Adaptation and Cell Integrity. Mol. Cell. Biol. 22: 5076-5088 [Abstract] [Full Text]  
  • Karpichev, I. V., Cornivelli, L., Small, G. M. (2002). Multiple Regulatory Roles of a Novel Saccharomyces cerevisiae Protein, Encoded by YOL002c, in Lipid and Phosphate Metabolism. J. Biol. Chem. 277: 19609-19617 [Abstract] [Full Text]  
  • Hyman, L. E., Kwon, E., Ghosh, S., McGee, J., Chachulska, A. M. B., Jackson, T., Baricos, W. H. (2002). Binding to Elongin C Inhibits Degradation of Interacting Proteins in Yeast. J. Biol. Chem. 277: 15586-15591 [Abstract] [Full Text]  
  • Edgington, N. P., Futcher, B. (2002). Relationship between the function and the location of G1 cyclins in S. cerevisiae. J. Cell Sci. 114: 4599-4611 [Abstract] [Full Text]  
  • Carroll, A. S., Bishop, A. C., DeRisi, J. L., Shokat, K. M., O'Shea, E. K. (2001). Chemical inhibition of the Pho85 cyclin-dependent kinase reveals a role in the environmental stress response. Proc. Natl. Acad. Sci. USA 98: 12578-12583 [Abstract] [Full Text]  
  • Anthony, C., Zong, Q., De Benedetti, A. (2001). Overexpression of eIF4E in Saccharomyces cerevisiae Causes Slow Growth and Decreased alpha -Factor Response through Alterations in CLN3 Expression. J. Biol. Chem. 276: 39645-39652 [Abstract] [Full Text]  
  • Baetz, K., Moffat, J., Haynes, J., Chang, M., Andrews, B. (2001). Transcriptional Coregulation by the Cell Integrity Mitogen-Activated Protein Kinase Slt2 and the Cell Cycle Regulator Swi4. Mol. Cell. Biol. 21: 6515-6528 [Abstract] [Full Text]  
  • Wang, Z., Wilson, W. A., Fujino, M. A., Roach, P. J. (2001). Antagonistic Controls of Autophagy and Glycogen Accumulation by Snf1p, the Yeast Homolog of AMP-Activated Protein Kinase, and the Cyclin-Dependent Kinase Pho85p. Mol. Cell. Biol. 21: 5742-5752 [Abstract] [Full Text]  
  • Schier, N., Liese, R., Fischer, R. (2001). A Pcl-Like Cyclin of Aspergillus nidulans Is Transcriptionally Activated by Developmental Regulators and Is Involved in Sporulation. Mol. Cell. Biol. 21: 4075-4088 [Abstract] [Full Text]  
  • Lenburg, M. E., O'Shea, E. K. (2001). Genetic Evidence for a Morphogenetic Function of the Saccharomyces cerevisiae Pho85 Cyclin-Dependent Kinase. Genetics 157: 39-51 [Abstract] [Full Text]  
  • Yao, S., Neiman, A., Prelich, G. (2000). BUR1 and BUR2 Encode a Divergent Cyclin-Dependent Kinase-Cyclin Complex Important for Transcription In Vivo. Mol. Cell. Biol. 20: 7080-7087 [Abstract] [Full Text]  
  • Tanaka, K., Okayama, H. (2000). A Pcl-like Cyclin Activates the Res2p-Cdc10p Cell Cycle "Start" Transcriptional Factor Complex in Fission Yeast. Mol. Biol. Cell 11: 2845-2862 [Abstract] [Full Text]  
  • Kaplun, L., Ivantsiv, Y., Kornitzer, D., Raveh, D. (2000). Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system. Proc. Natl. Acad. Sci. USA 97: 10077-10082 [Abstract] [Full Text]  
  • JIA, M. H., LAROSSA, R. A., LEE, J.-M., RAFALSKI, A., DEROSE, E., GONYE, G., XUE, Z. (2000). Global expression profiling of yeast treated with an inhibitor of amino acid biosynthesis, sulfometuron methyl. Physiol. Genomics 3: 83-92 [Abstract] [Full Text]  
  • Macpherson, N., Measday, V., Moore, L., Andrews, B. (2000). A Yeast taf17 Mutant Requires the Swi6 Transcriptional Activator for Viability and Shows Defects in Cell Cycle-Regulated Transcription. Genetics 154: 1561-1576 [Abstract] [Full Text]  
  • Le Roch, K., Sestier, C., Dorin, D., Waters, N., Kappes, B., Chakrabarti, D., Meijer, L., Doerig, C. (2000). Activation of a Plasmodium falciparum cdc2-related Kinase by Heterologous p25 and Cyclin H. FUNCTIONAL CHARACTERIZATION OF A P. FALCIPARUM CYCLIN HOMOLOGUE. J. Biol. Chem. 275: 8952-8958 [Abstract] [Full Text]  
  • Meimoun, A., Holtzman, T., Weissman, Z., McBride, H. J., Stillman, D. J., Fink, G. R., Kornitzer, D. (2000). Degradation of the Transcription Factor Gcn4 Requires the Kinase Pho85 and the SCFCDC4 Ubiquitin-Ligase Complex. Mol. Biol. Cell 11: 915-927 [Abstract] [Full Text]  
  • Huang, D., Patrick, G., Moffat, J., Tsai, L.-H., Andrews, B. (1999). Mammalian Cdk5 is a functional homologue of the budding yeast Pho85 cyclin-dependent protein kinase. Proc. Natl. Acad. Sci. USA 96: 14445-14450 [Abstract] [Full Text]  
  • Nishizawa, M., Kanaya, Y., Toh-e, A. (1999). Mouse Cyclin-dependent Kinase (Cdk) 5 Is a Functional Homologue of a Yeast Cdk, Pho85 Kinase. J. Biol. Chem. 274: 33859-33862 [Abstract] [Full Text]  
  • Baetz, K., Andrews, B. (1999). Regulation of Cell Cycle Transcription Factor Swi4 through Auto-Inhibition of DNA Binding. Mol. Cell. Biol. 19: 6729-6741 [Abstract] [Full Text]  
  • Wilson, W. A., Mahrenholz, A. M., Roach, P. J. (1999). Substrate Targeting of the Yeast Cyclin-Dependent Kinase Pho85p by the Cyclin Pcl10p. Mol. Cell. Biol. 19: 7020-7030 [Abstract] [Full Text]  
  • Ho, Y., Costanzo, M., Moore, L., Kobayashi, R., Andrews, B. J. (1999). Regulation of Transcription at the Saccharomyces cerevisiae Start Transition by Stb1, a Swi6-Binding Protein. Mol. Cell. Biol. 19: 5267-5278 [Abstract] [Full Text]  
  • Leza, M. A., Elion, E. A. (1999). POG1, a Novel Yeast Gene, Promotes Recovery From Pheromone Arrest via the G1 Cyclin CLN2. Genetics 151: 531-543 [Abstract] [Full Text]  
  • Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
  • Gustin, M. C., Albertyn, J., Alexander, M., Davenport, K. (1998). MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264-1300 [Abstract] [Full Text]  
  • Nishizawa, M., Kawasumi, M., Fujino, M., Toh-e, A. (1998). Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation. Mol. Biol. Cell 9: 2393-2405 [Abstract] [Full Text]  
  • Gray, N. S., Wodicka, L., Thunnissen, A. W., Norman, T. C., Kwon, S., Espinoza, F. H., Morgan, D. O., Barnes, G., LeClerc, S., Meijer, L., Kim, S., Lockhart, D. J., Schultz, P. G. (1998). Exploiting Chemical Libraries, Structure, and Genomics in the Search for Kinase Inhibitors. Science 281: 533-538 [Abstract] [Full Text]  
  • Huang, D., Moffat, J., Wilson, W. A., Moore, L., Cheng, C., Roach, P. J., Andrews, B. (1998). Cyclin Partners Determine Pho85 Protein Kinase Substrate Specificity In Vitro and In Vivo: Control of Glycogen Biosynthesis by Pcl8 and Pcl10. Mol. Cell. Biol. 18: 3289-3299 [Abstract] [Full Text]  
  • Wickert, S., Finck, M., Herz, B., Ernst, J. F. (1998). A Small Protein (Ags1p) and the Pho80p-Pho85p Kinase Complex Contribute to Aminoglycoside Antibiotic Resistance of the Yeast Saccharomyces cerevisiae. J. Bacteriol. 180: 1887-1894 [Abstract] [Full Text]  
  • Aerne, B. L., Johnson, A. L., Toyn, J. H., Johnston, L. H. (1998). Swi5 Controls a Novel Wave of Cyclin Synthesis in Late Mitosis. Mol. Biol. Cell 9: 945-956 [Abstract] [Full Text]  
  • (1998). An Essential Function of a Phosphoinositide-Specific Phospholipase C Is Relieved by Inhibition of a Cyclin-Dependent Protein Kinase in the Yeast Saccharomyces cerevisiae. Genetics 148: 33-48  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»