This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ye, X.
Right arrow Articles by Cavener, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ye, X.
Right arrow Articles by Cavener, D. R.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 03 1997, 1714-1721, Vol 17, No. 3
Copyright © 1997, American Society for Microbiology

Ultrabithorax and Antennapedia 5' untranslated regions promote developmentally regulated internal translation initiation

X Ye, P Fong, N Iizuka, D Choate and DR Cavener
Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA.

The 5' untranslated regions (UTRs) of the Drosophila Ubx and Antp genes were tested for their ability to promote cap-independent translation initiation. The Ubx and the Antp 5' UTR were inserted between the CAT and lacZ coding sequences in a dicistronic gene and tested for IRES activity in transgenic Drosophila. Northern analysis of the mRNAs showed the presence of the predicted full-length dicistronic mRNAs. High CAT activity was expressed from the first cistron from all of the dicistronic constructs introduced into the fly genome. The dicistronic transgenic strains bearing the Ubx and Antp IRES elements expressed significant levels of beta-galactosidase (betaGAL) from the second cistron whereas little or no betaGAL was expressed in the controls lacking the IRESs. In situ analysis of betaGAL expression in the transgenic strains indicates that expression of the second cistron is spatially and temporally regulated. Although the developmental patterns of expression directed by the Antp and Ubx IRESs overlap, they exhibit several differences indicating that these IRESs are not functionally equivalent.


This article has been cited by other articles:

  • Conte, C., Ainaoui, N., Delluc-Clavieres, A., Khoury, M. P., Azar, R., Pujol, F., Martineau, Y., Pyronnet, S., Prats, A.-C. (2009). Fibroblast growth factor 1 induced during myogenesis by a transcription-translation coupling mechanism. Nucleic Acids Res 37: 5267-5278 [Abstract] [Full Text]  
  • Shang, L. L., Pfahnl, A. E., Sanyal, S., Jiao, Z., Allen, J., Banach, K., Fahrenbach, J., Weiss, D., Taylor, W. R., Zafari, A. M., Dudley, S. C. Jr (2007). Human Heart Failure Is Associated With Abnormal C-Terminal Splicing Variants in the Cardiac Sodium Channel. Circ. Res. 101: 1146-1154 [Abstract] [Full Text]  
  • Huang, P., Pleasance, E. D., Maydan, J. S., Hunt-Newbury, R., O'Neil, N. J., Mah, A., Baillie, D. L., Marra, M. A., Moerman, D. G., Jones, S. J.M. (2007). Identification and analysis of internal promoters in Caenorhabditis elegans operons. Genome Res 17: 1478-1485 [Abstract] [Full Text]  
  • Marr, M. T. II, D'Alessio, J. A., Puig, O., Tjian, R. (2007). IRES-mediated functional coupling of transcription and translation amplifies insulin receptor feedback. Genes Dev. 21: 175-183 [Abstract] [Full Text]  
  • Kozak, M. (2005). A second look at cellular mRNA sequences said to function as internal ribosome entry sites. Nucleic Acids Res 33: 6593-6602 [Abstract] [Full Text]  
  • Churbanov, A., Rogozin, I. B., Babenko, V. N., Ali, H., Koonin, E. V. (2005). Evolutionary conservation suggests a regulatory function of AUG triplets in 5'-UTRs of eukaryotic genes. Nucleic Acids Res 33: 5512-5520 [Abstract] [Full Text]  
  • Komar, A. A., Hatzoglou, M. (2005). Internal Ribosome Entry Sites in Cellular mRNAs: Mystery of Their Existence. J. Biol. Chem. 280: 23425-23428 [Abstract] [Full Text]  
  • Komonyi, O., Papai, G., Enunlu, I., Muratoglu, S., Pankotai, T., Kopitova, D., Maroy, P., Udvardy, A., Boros, I. (2005). DTL, the Drosophila Homolog of PIMT/Tgs1 Nuclear Receptor Coactivator-interacting Protein/RNA Methyltransferase, Has an Essential Role in Development. J. Biol. Chem. 280: 12397-12404 [Abstract] [Full Text]  
  • Shang, L. L., Dudley, S. C. Jr. (2005). Tandem Promoters and Developmentally Regulated 5'- and 3'-mRNA Untranslated Regions of the Mouse Scn5a Cardiac Sodium Channel. J. Biol. Chem. 280: 933-940 [Abstract] [Full Text]  
  • HERNANDEZ, G., VAZQUEZ-PIANZOLA, P., SIERRA, J. M., RIVERA-POMAR, R. (2004). Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos. RNA 10: 1783-1797 [Abstract] [Full Text]  
  • Martineau, Y., Le Bec, C., Monbrun, L., Allo, V., Chiu, I.-M., Danos, O., Moine, H., Prats, H., Prats, A.-C. (2004). Internal Ribosome Entry Site Structural Motifs Conserved among Mammalian Fibroblast Growth Factor 1 Alternatively Spliced mRNAs. Mol. Cell. Biol. 24: 7622-7635 [Abstract] [Full Text]  
  • Negre, B., Ranz, J. M., Casals, F., Caceres, M., Ruiz, A. (2003). A New Split of the Hox Gene Complex in Drosophila: Relocation and Evolution of the Gene labial. Mol Biol Evol 20: 2042-2054 [Abstract] [Full Text]  
  • Meignin, C., Bailly, J.-L., Arnaud, F., Dastugue, B., Vaury, C. (2003). The 5' Untranslated Region and Gag product of Idefix, a Long Terminal Repeat-Retrotransposon from Drosophila melanogaster, Act Together To Initiate a Switch between Translated and Untranslated States of the Genomic mRNA. Mol. Cell. Biol. 23: 8246-8254 [Abstract] [Full Text]  
  • Boussadia, O., Niepmann, M., Creancier, L., Prats, A.-C., Dautry, F., Jacquemin-Sablon, H. (2003). Unr Is Required In Vivo for Efficient Initiation of Translation from the Internal Ribosome Entry Sites of both Rhinovirus and Poliovirus. J. Virol. 77: 3353-3359 [Abstract] [Full Text]  
  • Maier, D., Nagel, A. C., Preiss, A. (2002). Two isoforms of the Notch antagonist Hairless are produced by differential translation initiation. Proc. Natl. Acad. Sci. USA 99: 15480-15485 [Abstract] [Full Text]  
  • Shiroki, K., Ohsawa, C., Sugi, N., Wakiyama, M., Miura, K.-i., Watanabe, M., Suzuki, Y., Sugano, S. (2002). Internal ribosome entry site-mediated translation of Smad5 in vivo: requirement for a nuclear event. Nucleic Acids Res 30: 2851-2861 [Abstract] [Full Text]  
  • Hennecke, M., Kwissa, M., Metzger, K., Oumard, A., Kroger, A., Schirmbeck, R., Reimann, J., Hauser, H. (2001). Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs. Nucleic Acids Res 29: 3327-3334 [Abstract] [Full Text]  
  • Hellen, C. U.T., Sarnow, P. (2001). Internal ribosome entry sites in eukaryotic mRNA molecules. Genes Dev. 15: 1593-1612 [Full Text]  
  • Créancier, L., Mercier, P., Prats, A.-C., Morello, D. (2001). c-myc Internal Ribosome Entry Site Activity Is Developmentally Controlled and Subjected to a Strong Translational Repression in Adult Transgenic Mice. Mol. Cell. Biol. 21: 1833-1840 [Abstract] [Full Text]  
  • Owens, G. C., Chappell, S. A., Mauro, V. P., Edelman, G. M. (2001). Identification of two short internal ribosome entry sites selected from libraries of random oligonucleotides. Proc. Natl. Acad. Sci. USA 98: 1471-1476 [Abstract] [Full Text]  
  • Maraia, R. J., Intine, R. V. A. (2001). Recognition of Nascent RNA by the Human La Antigen: Conserved and Divergent Features of Structure and Function. Mol. Cell. Biol. 21: 367-379 [Full Text]  
  • Creancier, L., Morello, D., Mercier, P., Prats, A.-C. (2000). Fibroblast Growth Factor 2 Internal Ribosome Entry Site (Ires) Activity Ex Vivo and in Transgenic Mice Reveals a Stringent Tissue-Specific Regulation. JCB 150: 275-281 [Abstract] [Full Text]  
  • Holcik, M., Korneluk, R. G. (2000). Functional Characterization of the X-Linked Inhibitor of Apoptosis (XIAP) Internal Ribosome Entry Site Element: Role of La Autoantigen in XIAP Translation. Mol. Cell. Biol. 20: 4648-4657 [Abstract] [Full Text]  
  • Pozner, A., Goldenberg, D., Negreanu, V., Le, S.-Y., Elroy-Stein, O., Levanon, D., Groner, Y. (2000). Transcription-Coupled Translation Control of AML1/RUNX1 Is Mediated by Cap- and Internal Ribosome Entry Site-Dependent Mechanisms. Mol. Cell. Biol. 20: 2297-2307 [Abstract] [Full Text]  
  • Bai, C., Tolias, P. P. (2000). Genetic analysis of a La homolog in Drosophila melanogaster. Nucleic Acids Res 28: 1078-1084 [Abstract] [Full Text]  
  • Sella, O., Gerlitz, G., Le, S.-Y., Elroy-Stein, O. (1999). Differentiation-Induced Internal Translation of c-sis mRNA: Analysis of the cis Elements and Their Differentiation-Linked Binding to the hnRNP C Protein. Mol. Cell. Biol. 19: 5429-5440 [Abstract] [Full Text]  
  • Paz, I., Abramovitz, L., Choder, M. (1999). Starved Saccharomyces cerevisiae Cells Have the Capacity to Support Internal Initiation of Translation. J. Biol. Chem. 274: 21741-21745 [Abstract] [Full Text]  
  • Sluder, A. E., Mathews, S. W., Hough, D., Yin, V. P., Maina, C. V. (1999). The Nuclear Receptor Superfamily Has Undergone Extensive Proliferation and Diversification in Nematodes. Genome Res 9: 103-120 [Abstract] [Full Text]  
  • Huez, I., Créancier, L., Audigier, S., Gensac, M.-C., Prats, A.-C., Prats, H. (1998). Two Independent Internal Ribosome Entry Sites Are Involved in Translation Initiation of Vascular Endothelial Growth Factor mRNA. Mol. Cell. Biol. 18: 6178-6190 [Abstract] [Full Text]  
  • Stein, I., Itin, A., Einat, P., Skaliter, R., Grossman, Z., Keshet, E. (1998). Translation of Vascular Endothelial Growth Factor mRNA by Internal Ribosome Entry: Implications for Translation under Hypoxia. Mol. Cell. Biol. 18: 3112-3119 [Abstract] [Full Text]  
  • Gan, W., Celle, M. L., Rhoads, R. E. (1998). Functional Characterization of the Internal Ribosome Entry Site of eIF4G mRNA. J. Biol. Chem. 273: 5006-5012 [Abstract] [Full Text]  
  • Mertineit, C, Yoder, J., Taketo, T, Laird, D., Trasler, J., Bestor, T. (1998). Sex-specific exons control DNA methyltransferase in mammalian germ cells. Development 125: 889-897 [Abstract]  
  • Watada, H., Mirmira, R. G., Leung, J., German, M. S. (2000). Transcriptional and Translational Regulation of beta -Cell Differentiation Factor Nkx6.1. J. Biol. Chem. 275: 34224-34230 [Abstract] [Full Text]  
  • Nakai, Y., Nakai, M., Hayashi, H., Kagamiyama, H. (2001). Nuclear Localization of Yeast Nfs1p Is Required for Cell Survival. J. Biol. Chem. 276: 8314-8320 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»