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Mol. Cell. Biol., 05 1997, 2598-2604, Vol 17, No. 5
Copyright © 1997, American Society for Microbiology

Hormonal regulation of an islet-specific enhancer in the pancreatic homeobox gene STF-1

S Sharma, US Jhala, T Johnson, K Ferreri, J Leonard and M Montminy
Department of Biology, University of California, San Diego, La Jolla 92037, USA.

The homeobox protein STF-1 appears to function as a master control switch for expression of the pancreatic program during development. Here we characterize a composite enhancer which directs STF-1 expression to pancreatic islet cells via two functional elements that recognize the nuclear factors HNF-3beta and BETA-2. In keeping with their inhibitory effects on islet cell maturation, glucocorticoids were found to repress STF-1 gene expression by interfering with HNF-3beta activity on the islet-specific enhancer. Overexpression of HNF-3beta suppressed glucocorticoid receptor-mediated inhibition of the STF-1 gene, and our results suggest that the expansion of pancreatic islet precursor cells during development may be restricted by hormonal cues which regulate STF-1 gene expression.

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