This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neuteboom, S. T.
Right arrow Articles by Murre, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neuteboom, S. T.
Right arrow Articles by Murre, C.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 08 1997, 4696-4706, Vol 17, No. 8
Copyright © 1997, American Society for Microbiology

Pbx raises the DNA binding specificity but not the selectivity of antennapedia Hox proteins

ST Neuteboom and C Murre
Department of Biology, University of California, San Diego, La Jolla 92093, USA.

We have used a binding site selection strategy to determine the optimal binding sites for Pbx proteins by themselves and as heterodimeric partners with various Hox gene products. Among the Pbx proteins by themselves, only Pbx3 binds with high affinity, as a monomer or as a homodimer, to an optimal binding site, TGATTGATTTGAT. An inhibitory domain located N terminal of the Pbx1 homeodomain prevents intrinsic Pbx1 binding to this sequence. When complexed with Hoxc-6, each of the Pbx gene products binds the same consensus sequence, TGATTTAT, which differs from the site bound by Pbx3 alone. Three members of the Antennapedia family, Hoxc-6, Hoxb-7, and Hoxb-8, select the same binding site in conjunction with Pbx1. The affinities of these proteins as heterodimeric partners with Pbx1 for the selected optimal binding site are similar. However, the binding specificity of Hox proteins for optimal binding sites is increased, compared to nonspecific DNA, in the presence of Pbx proteins. Thus, while cooperative DNA binding involving heterodimers of Pbx and Hox gene products derived from members within the Antennapedia family does not increase binding site selectivity, DNA binding specificity of the Hox gene products is significantly enhanced in the presence of Pbx.


This article has been cited by other articles:

  • Pearson, J. C., Juarez, M. T., Kim, M., Drivenes, O., McGinnis, W. (2009). Multiple transcription factor codes activate epidermal wound-response genes in Drosophila. Proc. Natl. Acad. Sci. USA 106: 2224-2229 [Abstract] [Full Text]  
  • Hassan, M. Q., Tare, R., Lee, S. H., Mandeville, M., Weiner, B., Montecino, M., van Wijnen, A. J., Stein, J. L., Stein, G. S., Lian, J. B. (2007). HOXA10 Controls Osteoblastogenesis by Directly Activating Bone Regulatory and Phenotypic Genes. Mol. Cell. Biol. 27: 3337-3352 [Abstract] [Full Text]  
  • Wermuth, P. J., Buchberg, A. M. (2005). Meis1-mediated apoptosis is caspase dependent and can be suppressed by coexpression of HoxA9 in murine and human cell lines. Blood 105: 1222-1230 [Abstract] [Full Text]  
  • Remacle, S., Abbas, L., De Backer, O., Pacico, N., Gavalas, A., Gofflot, F., Picard, J. J., Rezsohazy, R. (2004). Loss of Function but No Gain of Function Caused by Amino Acid Substitutions in the Hexapeptide of Hoxa1 In Vivo. Mol. Cell. Biol. 24: 8567-8575 [Abstract] [Full Text]  
  • Pineault, N., Buske, C., Feuring-Buske, M., Abramovich, C., Rosten, P., Hogge, D. E., Aplan, P. D., Humphries, R. K. (2003). Induction of acute myeloid leukemia in mice by the human leukemia-specific fusion gene NUP98-HOXD13 in concert with Meis1. Blood 101: 4529-4538 [Abstract] [Full Text]  
  • Saleh, M., Rambaldi, I., Yang, X.-J., Featherstone, M. S. (2000). Cell Signaling Switches HOX-PBX Complexes from Repressors to Activators of Transcription Mediated by Histone Deacetylases and Histone Acetyltransferases. Mol. Cell. Biol. 20: 8623-8633 [Abstract] [Full Text]  
  • Veraksa, A, McGinnis, N, Li, X, Mohler, J, McGinnis, W (2000). Cap 'n' collar B cooperates with a small Maf subunit to specify pharyngeal development and suppress deformed homeotic function in the Drosophila head. Development 127: 4023-4037 [Abstract]  
  • Amendt, B. A., Sutherland, L. B., Russo, A. F. (1999). Multifunctional Role of the Pitx2 Homeodomain Protein C-Terminal Tail. Mol. Cell. Biol. 19: 7001-7010 [Abstract] [Full Text]  
  • Ryoo, H. D., Mann, R. S. (1999). The control of trunk Hox specificity and activity by Extradenticle. Genes Dev. 13: 1704-1716 [Abstract] [Full Text]  
  • Li, X, Veraksa, A, McGinnis, W (1999). A sequence motif distinct from Hox binding sites controls the specificity of a Hox response element. Development 126: 5581-5589 [Abstract]  
  • Amendt, B. A., Sutherland, L. B., Semina, E. V., Russo, A. F. (1998). The Molecular Basis of Rieger Syndrome. ANALYSIS OF PITX2 HOMEODOMAIN PROTEIN ACTIVITIES. J. Biol. Chem. 273: 20066-20072 [Abstract] [Full Text]  
  • Herzig, S., Fuzesi, L., Knepel, W. (2000). Heterodimeric Pbx-Prep1 Homeodomain Protein Binding to the Glucagon Gene Restricting Transcription in a Cell Type-dependent Manner. J. Biol. Chem. 275: 27989-27999 [Abstract] [Full Text]  
  • Pan, L., Xie, Y., Black, T. A., Jones, C. A., Pruitt, S. C., Gross, K. W. (2001). An Abd-B Class HOX{middle dot}PBX Recognition Sequence Is Required for Expression from the Mouse Ren-1c Gene. J. Biol. Chem. 276: 32489-32494 [Abstract] [Full Text]  
  • Liu, Y., MacDonald, R. J., Swift, G. H. (2001). DNA Binding and Transcriptional Activation by a PDX1{middle dot}PBX1b{middle dot}MEIS2b Trimer and Cooperation with a Pancreas-specific Basic Helix-Loop-Helix Complex. J. Biol. Chem. 276: 17985-17993 [Abstract] [Full Text]  
  • Eklund, E. A., Jalava, A., Kakar, R. (2000). Tyrosine Phosphorylation of HoxA10 Decreases DNA Binding and Transcriptional Repression during Interferon gamma -induced Differentiation of Myeloid Leukemia Cell Lines. J. Biol. Chem. 275: 20117-20126 [Abstract] [Full Text]  
  • Penkov, D., Tanaka, S., Di Rocco, G., Berthelsen, J., Blasi, F., Ramirez, F. (2000). Cooperative Interactions between PBX, PREP, and HOX Proteins Modulate the Activity of the alpha 2(V) Collagen (COL5A2) Promoter. J. Biol. Chem. 275: 16681-16689 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»