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Mol. Cell. Biol., Aug 1997, 4811-4819, Vol 17, No. 8
Copyright © 1997, American Society for Microbiology

The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo

LG Burns and CL Peterson
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01605, USA.

The Saccharomyces cerevisiae SWI-SNF complex is a 2-MDa protein assembly that is required for the function of many transcriptional activators. Here we describe experiments on the role of the SWI-SNF complex in activation of transcription by the yeast activator GAL4. We find that while SWI-SNF activity is not required for the GAL4 activator to bind to and activate transcription from nucleosome-free binding sites, the complex is required for GAL4 to bind to and function at low- affinity, nucleosomal binding sites in vivo. This SWI-SNF dependence can be overcome by (i) replacing the low-affinity sites with higher- affinity, consensus GAL4 binding sequences or (ii) placing the low- affinity sites into a nucleosome-free region. These results define the criteria for the SWI-SNF dependence of gene expression and provide the first in vivo evidence that the SWI-SNF complex can regulate gene expression by modulating the DNA binding of an upstream activator protein.


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