This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, T. E.
Right arrow Articles by Stuart, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, T. E.
Right arrow Articles by Stuart, K. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 1998, p. 6014-6022, Vol. 18, No. 10
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Association of Guide RNA Binding Protein gBP21 with Active RNA Editing Complexes in Trypanosoma brucei

Thomas E. Allen,1,2 Stefan Heidmann,3 RoseMary Reed,1,2 Peter J. Myler,1,2 H. Ulrich Göringer,4 and Kenneth D. Stuart1,2,*

Seattle Biomedical Research Institute, Seattle, Washington, 98109-1651,1 and Department of Pathobiology, University of Washington Seattle, Washington 98195,2 and Department of Genetics, University of Bayreuth, 95440 Bayreuth,3 and Laboratorium für Molekulare Biologie, Genzentrum der LMU München am MPI für Biochemie, 82152 Martinsried,4 Germany

Received 6 April 1998/Returned for modification 3 June 1998/Accepted 19 June 1998

RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitochondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of previously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA. These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.

* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 4 Nickerson St., Seattle, WA 98109-1651. Phone: (206) 284-8846, ext. 316. Fax: (206) 284-0313. E-mail: kstuart{at}u.washington.edu.

Molecular and Cellular Biology, October 1998, p. 6014-6022, Vol. 18, No. 10
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fisk, J. C., Presnyak, V., Ammerman, M. L., Read, L. K. (2009). Distinct and Overlapping Functions of MRP1/2 and RBP16 in Mitochondrial RNA Metabolism. Mol. Cell. Biol. 29: 5214-5225 [Abstract] [Full Text]  
  • Osato, D., Rogers, K., Guo, Q., Li, F., Richmond, G., Klug, F., Simpson, L. (2009). Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome. RNA 15: 1338-1344 [Abstract] [Full Text]  
  • Panigrahi, A. K., Zikova, A., Dalley, R. A., Acestor, N., Ogata, Y., Anupama, A., Myler, P. J., Stuart, K. D. (2008). Mitochondrial Complexes in Trypanosoma brucei: A Novel Complex and a Unique Oxidoreductase Complex. Mol. Cell. Proteomics 7: 534-545 [Abstract] [Full Text]  
  • Cifuentes-Rojas, C., Pavia, P., Hernandez, A., Osterwisch, D., Puerta, C., Cruz-Reyes, J. (2007). Substrate Determinants for RNA Editing and Editing Complex Interactions at a Site for Full-round U Insertion. J. Biol. Chem. 282: 4265-4276 [Abstract] [Full Text]  
  • Sacharidou, A., Cifuentes-Rojas, C., Halbig, K., Hernandez, A., Dangott, L. J., De Nova-Ocampo, M., Cruz-Reyes, J. (2006). RNA editing complex interactions with a site for full-round U deletion in Trypanosoma brucei. RNA 12: 1219-1228 [Abstract] [Full Text]  
  • Panigrahi, A. K., Ernst, N. L., Domingo, G. J., Fleck, M., Salavati, R., Stuart, K. D. (2006). Compositionally and functionally distinct editosomes in Trypanosoma brucei. RNA 12: 1038-1049 [Abstract] [Full Text]  
  • Salavati, R., Ernst, N. L., O'Rear, J., Gilliam, T., Tarun, S. Jr., Stuart, K. (2006). KREPA4, an RNA binding protein essential for editosome integrity and survival of Trypanosoma brucei. RNA 12: 819-831 [Abstract] [Full Text]  
  • Vondruskova, E., van den Burg, J., Zikova, A., Ernst, N. L., Stuart, K., Benne, R., Lukes, J. (2005). RNA Interference Analyses Suggest a Transcript-specific Regulatory Role for Mitochondrial RNA-binding Proteins MRP1 and MRP2 in RNA Editing and Other RNA Processing in Trypanosoma brucei. J. Biol. Chem. 280: 2429-2438 [Abstract] [Full Text]  
  • Penschow, J. L., Sleve, D. A., Ryan, C. M., Read, L. K. (2004). TbDSS-1, an Essential Trypanosoma brucei Exoribonuclease Homolog That Has Pleiotropic Effects on Mitochondrial RNA Metabolism. Eukaryot Cell 3: 1206-1216 [Abstract] [Full Text]  
  • Kang, X., Falick, A. M., Nelson, R. E., Gao, G., Rogers, K., Aphasizhev, R., Simpson, L. (2004). Disruption of the Zinc Finger Motifs in the Leishmania tarentolae LC-4 (=TbMP63) L-complex Editing Protein Affects the Stability of the L-complex. J. Biol. Chem. 279: 3893-3899 [Abstract] [Full Text]  
  • SIMPSON, L., APHASIZHEV, R., GAO, G., KANG, X. (2004). Mitochondrial proteins and complexes in Leishmania and Trypanosoma involved in U-insertion/deletion RNA editing. RNA 10: 159-170 [Abstract] [Full Text]  
  • Wang, B., Ernst, N. L., Palazzo, S. S., Panigrahi, A. K., Salavati, R., Stuart, K. (2003). TbMP44 Is Essential for RNA Editing and Structural Integrity of the Editosome in Trypanosoma brucei. Eukaryot Cell 2: 578-587 [Abstract] [Full Text]  
  • PANIGRAHI, A. K., SCHNAUFER, A., ERNST, N. L., WANG, B., CARMEAN, N., SALAVATI, R., STUART, K. (2003). Identification of novel components of Trypanosoma brucei editosomes. RNA 9: 484-492 [Abstract] [Full Text]  
  • SIMPSON, L., SBICEGO, S., APHASIZHEV, R. (2003). Uridine insertion/deletion RNA editing in trypanosome mitochondria: A complex business. RNA 9: 265-276 [Abstract] [Full Text]  
  • APHASIZHEV, R., APHASIZHEVA, I., NELSON, R. E., SIMPSON, L. (2003). A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components. RNA 9: 62-76 [Abstract] [Full Text]  
  • Cruz-Reyes, J., Zhelonkina, A. G., Huang, C. E., Sollner-Webb, B. (2002). Distinct Functions of Two RNA Ligases in Active Trypanosoma brucei RNA Editing Complexes. Mol. Cell. Biol. 22: 4652-4660 [Abstract] [Full Text]  
  • Igo, R. P. Jr., Lawson, S. D., Stuart, K. (2002). RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei. Mol. Cell. Biol. 22: 1567-1576 [Abstract] [Full Text]  
  • Muller, U. F., Goringer, H. U. (2002). Mechanism of the gBP21-mediated RNA/RNA annealing reaction: matchmaking and charge reduction. Nucleic Acids Res 30: 447-455 [Abstract] [Full Text]  
  • Blom, D., Burg, J. v. d., Breek, C. K. D., Speijer, D., Muijsers, A. O., Benne, R. (2001). Cloning and characterization of two guide RNA-binding proteins from mitochondria of Crithidia fasciculata: gBP27, a novel protein, and gBP29, the orthologue of Trypanosoma brucei gBP21. Nucleic Acids Res 29: 2950-2962 [Abstract] [Full Text]  
  • Cruz-Reyes, J., Zhelonkina, A., Rusche, L., Sollner-Webb, B. (2001). Trypanosome RNA Editing: Simple Guide RNA Features Enhance U Deletion 100-Fold. Mol. Cell. Biol. 21: 884-892 [Abstract] [Full Text]  
  • Panigrahi, A. K., Gygi, S. P., Ernst, N. L., Igo, R. P. Jr., Palazzo, S. S., Schnaufer, A., Weston, D. S., Carmean, N., Salavati, R., Aebersold, R., Stuart, K. D. (2001). Association of Two Novel Proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA Editing Complex. Mol. Cell. Biol. 21: 380-389 [Abstract] [Full Text]  
  • Igo, R. P. Jr., Palazzo, S. S., Burgess, M. L. K., Panigrahi, A. K., Stuart, K. (2000). Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing. Mol. Cell. Biol. 20: 8447-8457 [Abstract] [Full Text]  
  • Hayman, M. L., Read, L. K. (1999). Trypanosoma brucei RBP16 Is a Mitochondrial Y-box Family Protein with Guide RNA Binding Activity. J. Biol. Chem. 274: 12067-12074 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»