Viral Oncoproteins Discriminate between p53 and the p53 Homolog p73

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Molecular and Cellular Biology, November 1998, p. 6316-6324, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Viral Oncoproteins Discriminate between p53 and the p53 Homolog p73

Maria Carmen Marin,¹ Christine A. Jost,¹ Meredith S. Irwin,¹ James A. DeCaprio,¹ Daniel Caput,² and William G. Kaelin Jr.¹^,³^,^*

Dana-Farber Cancer Institute and Harvard Medical School¹ and Howard Hughes Medical Institute,³ Boston, Massachusetts 02115, and Sanofi Recherche, Labege, France²

Received 30 April 1998/Returned for modification 11 June 1998/Accepted 27 July 1998

p73 is a recently identified member of the p53 family. Previously it was shown that p73 can, when overproduced in p53-defectivetumor cells, activate p53-responsive promoters and induce apoptosis.In this report we describe the generation of anti-p73 monoclonalantibodies and confirm that two previously described p73 isoformsare produced in mammalian cells. Furthermore, we show that thesetwo isoforms can bind to canonical p53 DNA-binding sites in electrophoreticmobility shift assays. Despite the high degree of similarity betweenp53 and p73, we found that adenovirus E1B 55K, simian virus 40T, and human papillomavirus E6 do not physically interact withp73. The observation that viral oncoproteins discriminate betweenp53 and p73 suggests that the functions of these two proteinsmay differ under physiological conditions. Furthermore, they suggestthat inactivation of p73 may not be required for transformation.

^* Corresponding author. Mailing address: Dana-Farber Cancer Institute, 44 Binney St., Boston, MA 02115. Phone: (617) 632-3975.Fax: (617) 632-4760. E-mail: william_kaelin{at}dfci.harvard.edu.

Molecular and Cellular Biology, November 1998, p. 6316-6324, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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