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Molecular and Cellular Biology, November 1998, p. 6374-6386, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Yeast Los1p Has Properties of an Exportin-Like Nucleocytoplasmic Transport Factor for tRNA

Klaus Hellmuth,1 Denise M. Lau,1 F. Ralf Bischoff,2 Markus Künzler,1 Ed Hurt,1,* and George Simos1,*

Biochemie-Zentrum Heidelberg1 and Abteilung Molekulare Biologie der Mitose, Deutsches Krebsforschungszentrum,2 69120 Heidelberg, Germany

Received 30 March 1998/Returned for modification 18 May 1998/Accepted 5 August 1998

Saccharomyces cerevisiae Los1p, which is genetically linked to the nuclear pore protein Nsp1p and several tRNA biogenesis factors, was recently grouped into the family of importin/karyopherin-beta -like proteins on the basis of its sequence similarity. In a two-hybrid screen, we identified Nup2p as a nucleoporin interacting with Los1p. Subsequent purification of Los1p from yeast demonstrates its physical association not only with Nup2p but also with Nsp1p. By the use of the Gsp1p-G21V mutant, Los1p was shown to preferentially bind to the GTP-bound form of yeast Ran. Furthermore, overexpression of full-length or N-terminally truncated Los1p was shown to have dominant-negative effects on cell growth and different nuclear export pathways. Finally, Los1p could interact with Gsp1p-GTP, but only in the presence of tRNA, as revealed in an indirect in vitro binding assay. These data confirm the homology between Los1p and the recently identified human exportin for tRNA and reinforce the possibility of a role for Los1p in nuclear export of tRNA in yeast.

* Corresponding author. Mailing address: Biochemie-Zentrum Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany. Phone: 49-6221-546757. Fax: 49-6221-544369. E-mail for E.H.: cg5{at}ix.urz.uni-heidelberg.de. E-mail for G.S.: cg2{at}ix.urz.uni-heidelberg.de.

Molecular and Cellular Biology, November 1998, p. 6374-6386, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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