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Molecular and Cellular Biology, November 1998, p. 6560-6570, Vol. 18, No. 11
Department of Cell Biology and Anatomy, Mount
Sinai School of Medicine, New York, New York 10029
Received 2 June 1998/Returned for modification 14 July
1998/Accepted 28 July 1998
Two transcription factors, Oaf1p and Pip2p (Oaf2p), are key
components in the pathway by which several Saccharomyces
cerevisiae genes encoding peroxisomal proteins are activated in
the presence of a fatty acid such as oleate. By searching the S. cerevisiae genomic database for the consensus sequence that acts
as a target for these transcription factors, we identified 40 genes
that contain a putative Oaf1p-Pip2p binding site in their promoter
region. Quantitative Northern analysis confirmed that the expression of 22 of the genes identified is induced by oleate and that either one or
both of these transcription factors are required for the activation. In
addition to known peroxisomal proteins, the regulated genes encode
novel peroxisomal proteins, a mitochondrial protein, and proteins of
unknown location and function. We demonstrate that Oaf1p regulates
certain genes in the absence of Pip2p and that both of these
transcription factors play a role in maintaining the glucose-repressed
state of one gene. Furthermore, we provide evidence that the defined
consensus binding site is not required for the regulation of certain
oleate-responsive genes.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Global Regulatory Functions of Oaf1p and Pip2p
(Oaf2p), Transcription Factors That Regulate Genes Encoding Peroxisomal
Proteins in Saccharomyces cerevisiae
and
*
Corresponding author. Mailing address: Department of
Cell Biology and Anatomy, Box 1007, Mount Sinai School of Medicine, New York, NY 10029. Phone: (212) 241-0981. Fax: (212) 860-1174. E-mail: small{at}msvax.mssm.edu.
Permanent address: Centre of Bioengineering, Russian Academy of
Sciences, Moscow 117312, Russia.
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