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Molecular and Cellular Biology, November 1998, p. 6816-6825, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytoplasmic Dynein Intermediate-Chain Isoforms with Different Targeting Properties Created by Tissue-Specific Alternative Splicing

Dmitry I. Nurminsky,1,* Maria V. Nurminskaya,2 Elizaveta V. Benevolenskaya,3,4 Yury Y. Shevelyov,4 Daniel L. Hartl,1 and Vladimir A. Gvozdev4

Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, Massachusetts 021381; Department of Anatomy and Cell Biology, Tufts University School of Medicine, Boston, Massachusetts 0211112; University of Missouri---Columbia, Columbia, Missouri 652113; and Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia4

Received 13 April 1998/Returned for modification 5 June 1998/Accepted 14 August 1998

The intermediate chains (ICs) are the subunits of the cytoplasmic dynein that provide binding of the complex to cargo organelles through interaction of their N termini with dynactin. We present evidence that in Drosophila, the IC subunits are represented by at least 10 structural isoforms, created by the alternative splicing of transcripts from a unique Cdic gene. The splicing pattern is tissue specific. A constitutive set of four IC isoforms is expressed in all tissues tested; in addition, tissue-specific isoforms are found in the ovaries and nervous tissue. The structural variations between isoforms are limited to the N terminus of the IC molecule, where the interaction with dynactin takes place. This suggests differences in the dynactin-mediated organelle binding by IC isoforms. Accordingly, when transiently expressed in Drosophila Schneider-3 cells, the IC isoforms differ in their intracellular targeting properties from each other. A mechanism is proposed for the regulation of dynein binding to organelles through the changes in the content of the IC isoform pool.

* Corresponding author. Mailing address: Department of Organismic & Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 496-5540. Fax: (617) 496-5854. E-mail: dnurminsky{at}oeb.harvard.edu.

Molecular and Cellular Biology, November 1998, p. 6816-6825, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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