This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nurminsky, D. I.
Right arrow Articles by Gvozdev, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nurminsky, D. I.
Right arrow Articles by Gvozdev, V. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 1998, p. 6816-6825, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytoplasmic Dynein Intermediate-Chain Isoforms with Different Targeting Properties Created by Tissue-Specific Alternative Splicing

Dmitry I. Nurminsky,1,* Maria V. Nurminskaya,2 Elizaveta V. Benevolenskaya,3,4 Yury Y. Shevelyov,4 Daniel L. Hartl,1 and Vladimir A. Gvozdev4

Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, Massachusetts 021381; Department of Anatomy and Cell Biology, Tufts University School of Medicine, Boston, Massachusetts 0211112; University of Missouri---Columbia, Columbia, Missouri 652113; and Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia4

Received 13 April 1998/Returned for modification 5 June 1998/Accepted 14 August 1998

The intermediate chains (ICs) are the subunits of the cytoplasmic dynein that provide binding of the complex to cargo organelles through interaction of their N termini with dynactin. We present evidence that in Drosophila, the IC subunits are represented by at least 10 structural isoforms, created by the alternative splicing of transcripts from a unique Cdic gene. The splicing pattern is tissue specific. A constitutive set of four IC isoforms is expressed in all tissues tested; in addition, tissue-specific isoforms are found in the ovaries and nervous tissue. The structural variations between isoforms are limited to the N terminus of the IC molecule, where the interaction with dynactin takes place. This suggests differences in the dynactin-mediated organelle binding by IC isoforms. Accordingly, when transiently expressed in Drosophila Schneider-3 cells, the IC isoforms differ in their intracellular targeting properties from each other. A mechanism is proposed for the regulation of dynein binding to organelles through the changes in the content of the IC isoform pool.

* Corresponding author. Mailing address: Department of Organismic & Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 496-5540. Fax: (617) 496-5854. E-mail: dnurminsky{at}oeb.harvard.edu.

Molecular and Cellular Biology, November 1998, p. 6816-6825, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lo, K. W.-H., Kogoy, J. M., Rasoul, B. A., King, S. M., Pfister, K. K. (2007). Interaction of the DYNLT (TCTEX1/RP3) Light Chains and the Intermediate Chains Reveals Novel Intersubunit Regulation during Assembly of the Dynein Complex. J. Biol. Chem. 282: 36871-36878 [Abstract] [Full Text]  
  • Guo, Y., Jangi, S., Welte, M. A. (2005). Organelle-specific Control of Intracellular Transport: Distinctly Targeted Isoforms of the Regulator Klar. Mol. Biol. Cell 16: 1406-1416 [Abstract] [Full Text]  
  • Lee, W.-L., Kaiser, M. A., Cooper, J. A. (2005). The offloading model for dynein function: differential function of motor subunits. JCB 168: 201-207 [Abstract] [Full Text]  
  • Zhuang, Y., Ma, F., Li-Ling, J., Xu, X., Li, Y. (2003). Comparative Analysis of Amino Acid Usage and Protein Length Distribution Between Alternatively and Non-alternatively Spliced Genes Across Six Eukaryotic Genomes. Mol Biol Evol 20: 1978-1985 [Abstract] [Full Text]  
  • Boylan, K. L. M., Hays, T. S. (2002). The Gene for the Intermediate Chain Subunit of Cytoplasmic Dynein Is Essential in Drosophila. Genetics 162: 1211-1220 [Abstract] [Full Text]  
  • Lozovsky, E. R., Nurminsky, D., Wimmer, E. A., Hartl, D. L. (2002). Unexpected Stability of mariner Transgenes in Drosophila. Genetics 160: 527-535 [Abstract] [Full Text]  
  • Yoder, J. H., Han, M. (2001). Cytoplasmic Dynein Light Intermediate Chain Is Required for Discrete Aspects of Mitosis in Caenorhabditis elegans. Mol. Biol. Cell 12: 2921-2933 [Abstract] [Full Text]  
  • Boylan, K., Serr, M., Hays, T. (2000). A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex. Mol. Biol. Cell 11: 3791-3803 [Abstract] [Full Text]  
  • Gross, S. P., Welte, M. A., Block, S. M., Wieschaus, E. F. (2000). Dynein-Mediated Cargo Transport in Vivo: A Switch Controls Travel Distance. JCB 148: 945-956 [Abstract] [Full Text]  
  • Habura, A., Tikhonenko, I., Chisholm, R. L., Koonce, M. P. (1999). Interaction Mapping of a Dynein Heavy Chain. IDENTIFICATION OF DIMERIZATION AND INTERMEDIATE-CHAIN BINDING DOMAINS. J. Biol. Chem. 274: 15447-15453 [Abstract] [Full Text]  
  • Roghi, C, Allan, V. (1999). Dynamic association of cytoplasmic dynein heavy chain 1a with the Golgi apparatus and intermediate compartment. J. Cell Sci. 112: 4673-4685 [Abstract]  
  • Lo, K. W.-H., Naisbitt, S., Fan, J.-S., Sheng, M., Zhang, M. (2001). The 8-kDa Dynein Light Chain Binds to Its Targets via a Conserved (K/R)XTQT Motif. J. Biol. Chem. 276: 14059-14066 [Abstract] [Full Text]  
  • Mok, Y.-K., Lo, K. W.-H., Zhang, M. (2001). Structure of Tctex-1 and Its Interaction with Cytoplasmic Dynein Intermediate Chain. J. Biol. Chem. 276: 14067-14074 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»