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Molecular and Cellular Biology, November 1998, p. 6826-6838, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nuclear mRNA Export Requires Complex Formation between Mex67p and Mtr2p at the Nuclear Pores

Helena Santos-Rosa,1 Horacio Moreno,1 George Simos,1 Alexandra Segref,1 Birthe Fahrenkrog,2 Nelly Panté,2,dagger and Ed Hurt1,*

Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany,1 and Maurice E. Müller Institute, Biozentrum University of Basel, Basel, Switzerland2

Received 20 April 1998/Returned for modification 25 May 1998/Accepted 4 August 1998

We have identified between Mex67p and Mtr2p a complex which is essential for mRNA export. This complex, either isolated from yeast or assembled in Escherichia coli, can bind in vitro to RNA through Mex67p. In vivo, Mex67p requires Mtr2p for association with the nuclear pores, which can be abolished by mutating either MEX67 or MTR2. In all cases, detachment of Mex67p from the pores into the cytoplasm correlates with a strong inhibition of mRNA export. At the nuclear pores, Nup85p represents one of the targets with which the Mex67p-Mtr2p complex interacts. Thus, Mex67p and Mtr2p constitute a novel mRNA export complex which can bind to RNA via Mex67p and which interacts with nuclear pores via Mtr2p.

* Corresponding author. Mailing address: Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany. Phone: 49-6221-54 41 73. Fax: 49-6221-54 43 69. E-mail: cg5{at}ix.urz.uni-heidelberg.de.

dagger Present address: Institute of Biochemistry, ETH-Zürich, Switzerland.

Molecular and Cellular Biology, November 1998, p. 6826-6838, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Zhao, J., Hyman, L., Moore, C. (1999). Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis. Microbiol. Mol. Biol. Rev. 63: 405-445 [Abstract] [Full Text]  
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  • Braun, I. C., Herold, A., Rode, M., Conti, E., Izaurralde, E. (2001). Overexpression of TAP/p15 Heterodimers Bypasses Nuclear Retention and Stimulates Nuclear mRNA Export. J. Biol. Chem. 276: 20536-20543 [Abstract] [Full Text]  
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  • Westberg, C., Yang, J.-P., Tang, H., Reddy, T. R., Wong-Staal, F. (2000). A Novel Shuttle Protein Binds to RNA Helicase A and Activates the Retroviral Constitutive Transport Element. J. Biol. Chem. 275: 21396-21401 [Abstract] [Full Text]  


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