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Molecular and Cellular Biology, December 1998, p. 6879-6884, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Embryo Deadenylation Element-Dependent Deadenylation Is Enhanced by a cis Element Containing AUU Repeats

Yann Audic, Francis Omilli, and H. Beverley Osborne*

CNRS UPR 41, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France

Received 28 May 1998/Returned for modification 23 June 1998/Accepted 9 September 1998

The deadenylation of maternal mRNAs in the Xenopus embryo is a sequence-specific process. One cis element that targets maternal mRNAs for deadenylation after fertilization is the embryo deadenylation element (EDEN). This element, composed of U/R repeats, is specifically bound by a protein, EDEN-BP. In the present study we show that the rate at which an RNA containing an EDEN is deadenylated can be increased by the presence of an additional cis element composed of three AUU repeats. This effect was observed for a natural EDEN (c-mos) and two synthetic EDENs. Hence, the enhancement of EDEN-dependent deadenylation conferred by the (AUU)3 motif is not due to an interaction with a particular EDEN sequence. Mutation of the (AUU)3 motif abrogated the enhancement of EDEN-dependent deadenylation. These data indicate that the rate at which a specific maternal mRNA is deadenylated in Xenopus embryos is probably defined by a cross talk between multiple cis elements.

* Corresponding author. Mailing address: CNRS UPR 41, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France. Phone: 33 (0)299 28 61 13. Fax: 33 (0)299 28 16 49. E-mail: Beverley.Osborne{at}univ-rennes1.fr.

Molecular and Cellular Biology, December 1998, p. 6879-6884, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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