This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, E. J.
Right arrow Articles by Kaiser, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, E. J.
Right arrow Articles by Kaiser, C. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 1998, p. 7139-7146, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Link between Secretion and Pre-mRNA Processing Defects in Saccharomyces cerevisiae and the Identification of a Novel Splicing Gene, RSE1

Esther J. Chen, Alison R. Frand, Elizabeth Chitouras, and Chris A. Kaiser*

Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 15 May 1998/Returned for modification 26 June 1998/Accepted 28 August 1998

Secretory proteins in eukaryotic cells are transported to the cell surface via the endoplasmic reticulum (ER) and the Golgi apparatus by membrane-bounded vesicles. We screened a collection of temperature-sensitive mutants of Saccharomyces cerevisiae for defects in ER-to-Golgi transport. Two of the genes identified in this screen were PRP2, which encodes a known pre-mRNA splicing factor, and RSE1, a novel gene that we show to be important for pre-mRNA splicing. Both prp2-13 and rse1-1 mutants accumulate the ER forms of invertase and the vacuolar protease CPY at restrictive temperature. The secretion defect in each mutant can be suppressed by increasing the amount of SAR1, which encodes a small GTPase essential for COPII vesicle formation from the ER, or by deleting the intron from the SAR1 gene. These data indicate that a failure to splice SAR1 pre-mRNA is the specific cause of the secretion defects in prp2-13 and rse1-1. Moreover, these data imply that Sar1p is a limiting component of the ER-to-Golgi transport machinery and suggest a way that secretory pathway function might be coordinated with the amount of gene expression in a cell.

* Corresponding author. Mailing address: Department of Biology, Room 68-533, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-9804. Fax: (617) 253-8699. E-mail: ckaiser{at}mit.edu.

Molecular and Cellular Biology, December 1998, p. 7139-7146, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garas, M., Dichtl, B., Keller, W. (2008). The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis. RNA 14: 2671-2684 [Abstract] [Full Text]  
  • Vincent, K., Wang, Q., Jay, S., Hobbs, K., Rymond, B. C. (2003). Genetic Interactions With CLF1 Identify Additional Pre-mRNA Splicing Factors and a Link Between Activators of Yeast Vesicular Transport and Splicing. Genetics 164: 895-907 [Abstract] [Full Text]  
  • Faustino, N. A., Cooper, T. A. (2003). Pre-mRNA splicing and human disease. Genes Dev. 17: 419-437 [Full Text]  
  • Burns, C. G., Ohi, R., Mehta, S., O'Toole, E. T., Winey, M., Clark, T. A., Sugnet, C. W., Ares, M. Jr., Gould, K. L. (2002). Removal of a Single {alpha}-Tubulin Gene Intron Suppresses Cell Cycle Arrest Phenotypes of Splicing Factor Mutations in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 801-815 [Abstract] [Full Text]  
  • McKie, A. B., McHale, J. C., Keen, T. J., Tarttelin, E. E., Goliath, R., van Lith-Verhoeven, J. J.C., Greenberg, J., Ramesar, R. S., Hoyng, C. B., Cremers, F. P.M., Mackey, D. A., Bhattacharya, S. S., Bird, A. C., Markham, A. F., Inglehearn, C. F. (2001). Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13). Hum Mol Genet 10: 1555-1562 [Abstract] [Full Text]  
  • Libri, D., Graziani, N., Saguez, C., Boulay, J. (2001). Multiple roles for the yeast SUB2/yUAP56 gene in splicing. Genes Dev. 15: 36-41 [Abstract] [Full Text]  
  • Nanduri, J., Mitra, S., Andrei, C., Liu, Y., Yu, Y., Hitomi, M., Tartakoff, A. M. (1999). An Unexpected Link between the Secretory Path and the Organization of the Nucleus. J. Biol. Chem. 274: 33785-33789 [Abstract] [Full Text]  
  • Das, B. K., Xia, L., Palandjian, L., Gozani, O., Chyung, Y., Reed, R. (1999). Characterization of a Protein Complex Containing Spliceosomal Proteins SAPs 49, 130, 145, and 155. Mol. Cell. Biol. 19: 6796-6802 [Abstract] [Full Text]  
  • Zhao, J., Hyman, L., Moore, C. (1999). Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis. Microbiol. Mol. Biol. Rev. 63: 405-445 [Abstract] [Full Text]  
  • Awasthi, S., Palmer, R., Castro, M., Mobarak, C. D., Ruby, S. W. (2001). New Roles for the Snp1 and Exo84 Proteins in Yeast Pre-mRNA Splicing. J. Biol. Chem. 276: 31004-31015 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»