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Molecular and Cellular Biology, December 1998, p. 7294-7303, Vol. 18, No. 12
Department of Genetics, Roswell Park Cancer
Institute, Buffalo, New York 14263
Received 3 June 1998/Returned for modification 3 August
1998/Accepted 19 August 1998
Previous investigations have shown that the fission yeast,
Schizosaccharomyces pombe, has DNA replication origins (500 to 1500 bp) that are larger than those in the budding yeast,
Saccharomyces cerevisiae (100 to 150 bp). Deletion and
linker substitution analyses of two fission yeast origins revealed that
they contain multiple important regions with AT-rich asymmetric
(abundant A residues in one strand and T residues in the complementary
strand) sequence motifs. In this work we present the characterization
of a third fission yeast replication origin, ars3001, which
is relatively small (~570 bp) and responsible for replication of
ribosomal DNA. Like previously studied fission yeast origins,
ars3001 contains multiple important regions. The three most
important of these regions resemble each other in several ways: each
region is essential for origin function and is at least partially
orientation dependent, each region contains similar clusters of
A+T-rich asymmetric sequences, and the regions can partially substitute
for each other. These observations suggest that ars3001
function requires synergistic interactions between domains binding
similar proteins. It is likely that this requirement extends to other
fission yeast origins, explaining why such origins are larger than
those of budding yeast.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Multiple Orientation-Dependent, Synergistically
Interacting, Similar Domains in the Ribosomal DNA Replication
Origin of the Fission Yeast, Schizosaccharomyces
pombe
*
Corresponding author. Mailing address: Department of
Genetics, Roswell Park Cancer Institute, Elm & Carlton Streets,
Buffalo, NY 14263-0001. Phone: (716) 845-3047. Fax: (716) 845-8126. E-mail: huberman{at}acsu.buffalo.edu.
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