This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moore, S. P.
Right arrow Articles by Garfinkel, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moore, S. P.
Right arrow Articles by Garfinkel, D. J.

 Previous Article  |  Next Article 

Mol Cell Biol, February 1998, p. 1105-1114, Vol. 18, No. 2
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Ty1 Integrase Nuclear Localization Signal Required for Retrotransposition

Sharon P. Moore, Lori A. Rinckel,dagger and David J. Garfinkel*

Gene Regulation and Chromosome Biology Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201

Received 5 September 1997/Returned for modification 22 October 1997/Accepted 7 November 1997

Ty1 retrotransposition in Saccharomyces cerevisiae requires integrase (IN)-mediated insertion of Ty1 cDNA into the host genome. The transposition components are assembled in the cytoplasm and must cross the nuclear envelope to reach the genomic target, since, unlike animal cell nuclear membranes, the yeast cell nuclear membrane remains intact throughout the cell cycle. We have identified a bipartite nuclear localization signal (NLS) in IN required for Ty1 transposition (Ty1 IN) that directs IN to the nucleus. Mutations in the NLS that specifically abolish nuclear localization inactivate transpositional integration but do not affect reverse transcription, protein processing, or catalytic activity in vitro. No additional Ty1-encoded proteins are required for IN nuclear localization. Intragenic complementation experiments suggest that Ty1 IN functions as a multimer and contains two distinct domains, one required for integration and the other for nuclear localization. Nuclear targeting of the preintegration complex by an IN NLS may prove to be a general strategy used by retrotransposons and retroviruses that infect nondividing cells.

* Corresponding author. Mailing address: Gene Regulation and Chromosome Biology Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD 21702-1201. Phone: (301) 846-5604. Fax: (301) 846-6911. E-mail: garfinke{at}ncifcrf.gov.

dagger Present address: Department of Medicine, University of Maryland School of Medicine, Veterans Administration Medical Center, Baltimore, Maryland.




This article has been cited by other articles:

  • Malagon, F., Jensen, T. H. (2008). The T Body, a New Cytoplasmic RNA Granule in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 6022-6032 [Abstract] [Full Text]  
  • McLane, L. M., Pulliam, K. F., Devine, S. E., Corbett, A. H. (2008). The Ty1 integrase protein can exploit the classical nuclear protein import machinery for entry into the nucleus. Nucleic Acids Res 36: 4317-4326 [Abstract] [Full Text]  
  • Garfinkel, D. J., Stefanisko, K. M., Nyswaner, K. M., Moore, S. P., Oh, J., Hughes, S. H. (2006). Retrotransposon Suicide: Formation of Ty1 Circles and Autointegration via a Central DNA Flap. J. Virol. 80: 11920-11934 [Abstract] [Full Text]  
  • Wilhelm, M., Wilhelm, F.-X. (2006). Cooperation between Reverse Transcriptase and Integrase during Reverse Transcription and Formation of the Preintegrative Complex of Ty1.. Eukaryot Cell 5: 1760-1769 [Abstract] [Full Text]  
  • Wilhelm, M., Wilhelm, F.-X. (2005). Role of Integrase in Reverse Transcription of the Saccharomyces cerevisiae Retrotransposon Ty1. Eukaryot Cell 4: 1057-1065 [Abstract] [Full Text]  
  • Garfinkel, D. J., Nyswaner, K. M., Stefanisko, K. M., Chang, C., Moore, S. P. (2005). Ty1 Copy Number Dynamics in Saccharomyces. Genetics 169: 1845-1857 [Abstract] [Full Text]  
  • Aye, M., Irwin, B., Beliakova-Bethell, N., Chen, E., Garrus, J., Sandmeyer, S. (2004). Host Factors That Affect Ty3 Retrotransposition in Saccharomyces cerevisiae. Genetics 168: 1159-1176 [Abstract] [Full Text]  
  • Llano, M., Vanegas, M., Fregoso, O., Saenz, D., Chung, S., Peretz, M., Poeschla, E. M. (2004). LEDGF/p75 Determines Cellular Trafficking of Diverse Lentiviral but Not Murine Oncoretroviral Integrase Proteins and Is a Component of Functional Lentiviral Preintegration Complexes. J. Virol. 78: 9524-9537 [Abstract] [Full Text]  
  • Radford, S. J., Boyle, M. L., Sheely, C. J., Graham, J., Haeusser, D. P., Zimmerman, L., Keeney, J. B. (2004). Increase in Ty1 cDNA Recombination in Yeast sir4 Mutant Strains at High Temperature. Genetics 168: 89-101 [Abstract] [Full Text]  
  • Griffith, J. L., Coleman, L. E., Raymond, A. S., Goodson, S. G., Pittard, W. S., Tsui, C., Devine, S. E. (2003). Functional Genomics Reveals Relationships Between the Retrovirus-Like Ty1 Element and Its Host Saccharomyces cerevisiae. Genetics 164: 867-879 [Abstract] [Full Text]  
  • Peterson-Burch, B. D., Voytas, D. F. (2002). Genes of the Pseudoviridae (Ty1/copia Retrotransposons). Mol Biol Evol 19: 1832-1845 [Abstract] [Full Text]  
  • Lin, S. S., Nymark-McMahon, M. H., Yieh, L., Sandmeyer, S. B. (2001). Integrase Mediates Nuclear Localization of Ty3. Mol. Cell. Biol. 21: 7826-7838 [Abstract] [Full Text]  
  • Xie, W., Gai, X., Zhu, Y., Zappulla, D. C., Sternglanz, R., Voytas, D. F. (2001). Targeting of the Yeast Ty5 Retrotransposon to Silent Chromatin Is Mediated by Interactions between Integrase and Sir4p. Mol. Cell. Biol. 21: 6606-6614 [Abstract] [Full Text]  
  • Bryk, M., Banerjee, M., Conte, D. Jr., Curcio, M. J. (2001). The Sgs1 Helicase of Saccharomyces cerevisiae Inhibits Retrotransposition of Ty1 Multimeric Arrays. Mol. Cell. Biol. 21: 5374-5388 [Abstract] [Full Text]  
  • Lawler, J. F. Jr., Merkulov, G. V., Boeke, J. D. (2001). Frameshift Signal Transplantation and the Unambiguous Analysis of Mutations in the Yeast Retrotransposon Ty1 Gag-Pol Overlap Region. J. Virol. 75: 6769-6775 [Abstract] [Full Text]  
  • Merkulov, G. V., Lawler, J. F. Jr., Eby, Y., Boeke, J. D. (2001). Ty1 Proteolytic Cleavage Sites Are Required for Transposition: All Sites Are Not Created Equal. J. Virol. 75: 638-644 [Abstract] [Full Text]  
  • Lee, B.-S., Bi, L., Garfinkel, D. J., Bailis, A. M. (2000). Nucleotide Excision Repair/TFIIH Helicases Rad3 and Ssl2 Inhibit Short-Sequence Recombination and Ty1 Retrotransposition by Similar Mechanisms. Mol. Cell. Biol. 20: 2436-2445 [Abstract] [Full Text]  
  • Balasundaram, D., Benedik, M. J., Morphew, M., Dang, V.-D., Levin, H. L. (1999). Nup124p Is a Nuclear Pore Factor of Schizosaccharomyces pombe That Is Important for Nuclear Import and Activity of Retrotransposon Tf1. Mol. Cell. Biol. 19: 5768-5784 [Abstract] [Full Text]  
  • Dang, V. D., Benedik, M. J., Ekwall, K., Choi, J., Allshire, R. C., Levin, H. L. (1999). A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast. Mol. Cell. Biol. 19: 2351-2365 [Abstract] [Full Text]  
  • Nymark-McMahon, M. H., Sandmeyer, S. B. (1999). Mutations in Nonconserved Domains of Ty3 Integrase Affect Multiple Stages of the Ty3 Life Cycle. J. Virol. 73: 453-465 [Abstract] [Full Text]  
  • Kenna, M. A., Brachmann, C. B., Devine, S. E., Boeke, J. D. (1998). Invading the Yeast Nucleus: a Nuclear Localization Signal at the C Terminus of Ty1 Integrase Is Required for Transposition In Vivo. Mol. Cell. Biol. 18: 1115-1124 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»