This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Erman, B.
Right arrow Articles by Sen, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Erman, B.
Right arrow Articles by Sen, R.

 Previous Article  |  Next Article 

Mol Cell Biol, March 1998, p. 1322-1330, Vol. 18, No. 3
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

ETS-Core Binding Factor: a Common Composite Motif in Antigen Receptor Gene Enhancers

Batu Erman,1 Marta Cortes,1 Barbara S. Nikolajczyk,1 Nancy A. Speck,2 and Ranjan Sen1,*

Rosenstiel Research Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254,1 and Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 037552

Received 15 August 1997/Returned for modification 23 September 1997/Accepted 9 December 1997

A tripartite domain of the murine immunoglobulin µ heavy-chain enhancer contains the µA and µB elements that bind ETS proteins and the µE3 element that binds leucine zipper-containing basic helix-loop-helix (bHLH-zip) factors. Analysis of the corresponding region of the human µ enhancer revealed high conservation of the µA and µB motifs but a striking absence of the µE3 element. Instead of bHLH-zip proteins, we found that the human enhancer bound core binding factor (CBF) between the µA and µB elements; CBF binding was shown to be a common feature of both murine and human enhancers. Furthermore, mutant enhancers that bound prototypic bHLH-zip proteins but not CBF did not activate transcription in B cells, and conversely, CBF transactivated the murine enhancer in nonlymphoid cells. Taking these data together with the earlier analysis of T-cell-specific enhancers, we propose that ETS-CBF is a common composite element in the regulation of antigen receptor genes. In addition, these studies identify the first B-cell target of CBF, a protein that has been implicated in the development of childhood pre-B-cell leukemias.

* Corresponding author. Mailing address: Rosenstiel Research Center and Department of Biology, Brandeis University, Waltham, MA 02254. Phone: (781) 736-2455. Fax: (781) 736-2405. E-mail: sen{at}binah.cc.brandeis.edu.




This article has been cited by other articles:

  • Achour, I., Cavelier, P., Tichit, M., Bouchier, C., Lafaye, P., Rougeon, F. (2008). Tetrameric and Homodimeric Camelid IgGs Originate from the Same IgH Locus. J. Immunol. 181: 2001-2009 [Abstract] [Full Text]  
  • Hikima, J.-i., Cioffi, C. C., Middleton, D. L., Wilson, M. R., Miller, N. W., Clem, L. W., Warr, G. W. (2004). Evolution of Transcriptional Control of the IgH Locus: Characterization, Expression, and Function of TF12/HEB Homologs of the Catfish. J. Immunol. 173: 5476-5484 [Abstract] [Full Text]  
  • Brown, C., Gaspar, J., Pettit, A., Lee, R., Gu, X., Wang, H., Manning, C., Voland, C., Goldring, S. R., Goldring, M. B., Libermann, T. A., Gravallese, E. M., Oettgen, P. (2004). ESE-1 Is a Novel Transcriptional Mediator of Angiopoietin-1 Expression in the Setting of Inflammation. J. Biol. Chem. 279: 12794-12803 [Abstract] [Full Text]  
  • McCarthy, K. M., McDevit, D., Andreucci, A., Reeves, R., Nikolajczyk, B. S. (2003). HMGA1 Co-activates Transcription in B Cells through Indirect Association with DNA. J. Biol. Chem. 278: 42106-42114 [Abstract] [Full Text]  
  • Cutrona, G., Carpaneto, E. M., Ponzanelli, A., Ulivi, M., Millo, E., Scarfi, S., Roncella, S., Benatti, U., Boffa, L. C., Ferrarini, M. (2003). Inhibition of the Translocated c-myc in Burkitt's Lymphoma by a PNA Complementary to the E{micro} Enhancer. Cancer Res. 63: 6144-6148 [Abstract] [Full Text]  
  • Schwieger, M., Lohler, J., Friel, J., Scheller, M., Horak, I., Stocking, C. (2002). AML1-ETO Inhibits Maturation of Multiple Lymphohematopoietic Lineages and Induces Myeloblast Transformation in Synergy with ICSBP Deficiency. JEM 196: 1227-1240 [Abstract] [Full Text]  
  • Spender, L. C., Cornish, G. H., Sullivan, A., Farrell, P. J. (2002). Expression of Transcription Factor AML-2 (RUNX3, CBF{alpha}-3) Is Induced by Epstein-Barr Virus EBNA-2 and Correlates with the B-Cell Activation Phenotype. J. Virol. 76: 4919-4927 [Abstract] [Full Text]  
  • Shi, M.-J., Park, S.-R., Kim, P.-H., Stavnezer, J. (2001). Roles of Ets proteins, NF-{{kappa}}B and nocodazole in regulating induction of transcription of mouse germline Ig {{alpha}} RNA by transforming growth factor-{beta}1. Int Immunol 13: 733-746 [Abstract] [Full Text]  
  • Tripathi, R. K., Mathieu, N., Spicuglia, S., Payet, D., Verthuy, C., Bouvier, G., Depetris, D., Mattei, M.-G., HempeL, W. M., Ferrier, P. (2000). Definition of a T-Cell Receptor beta Gene Core Enhancer of V(D)J Recombination by Transgenic Mapping. Mol. Cell. Biol. 20: 42-53 [Abstract] [Full Text]  
  • Goetz, T. L., Gu, T.-L., Speck, N. A., Graves, B. J. (2000). Auto-Inhibition of Ets-1 Is Counteracted by DNA Binding Cooperativity with Core-Binding Factor alpha 2. Mol. Cell. Biol. 20: 81-90 [Abstract] [Full Text]  
  • Gu, T.-L., Goetz, T. L., Graves, B. J., Speck, N. A. (2000). Auto-Inhibition and Partner Proteins, Core-Binding Factor beta (CBFbeta ) and Ets-1, Modulate DNA Binding by CBFalpha 2 (AML1). Mol. Cell. Biol. 20: 91-103 [Abstract] [Full Text]  
  • Rao, S., Garrett-Sinha, L. A., Yoon, J., Simon, M. C. (1999). The Ets Factors PU.1 and Spi-B Regulate the Transcription in Vivo of P2Y10, a Lymphoid Restricted Heptahelical Receptor. J. Biol. Chem. 274: 34245-34252 [Abstract] [Full Text]  
  • Libermann, T. A., Pan, Z., Akbarali, Y., Hetherington, C. J., Boltax, J., Yergeau, D. A., Zhang, D.-E. (1999). AML1 (CBFalpha 2) Cooperates with B Cell-specific Activating Protein (BSAP/PAX5) in Activation of the B Cell-specific BLK Gene Promoter. J. Biol. Chem. 274: 24671-24676 [Abstract] [Full Text]  
  • Lewis, A. F., Stacy, T., Green, W. R., Taddesse-Heath, L., Hartley, J. W., Speck, N. A. (1999). Core-Binding Factor Influences the Disease Specificity of Moloney Murine Leukemia Virus. J. Virol. 73: 5535-5547 [Abstract] [Full Text]  
  • Rao, S., Matsumura, A., Yoon, J., Simon, M. C. (1999). SPI-B Activates Transcription via a Unique Proline, Serine, and Threonine Domain and Exhibits DNA Binding Affinity Differences from PU.1. J. Biol. Chem. 274: 11115-11124 [Abstract] [Full Text]  
  • Shi, M.-J., Stavnezer, J. (1998). CBF{alpha}3 (AML2) Is Induced by TGF-{beta}1 to Bind and Activate the Mouse Germline Ig {alpha} Promoter. J. Immunol. 161: 6751-6760 [Abstract] [Full Text]  
  • Dang, W., Nikolajczyk, B. S., Sen, R. (1998). Exploring Functional Redundancy in the Immunoglobulin µ Heavy-Chain Gene Enhancer. Mol. Cell. Biol. 18: 6870-6878 [Abstract] [Full Text]  
  • Cioffi, C. C., Middleton, D. L., Wilson, M. R., Miller, N. W., Clem, L. W., Warr, G. W. (2001). An IgH Enhancer That Drives Transcription through Basic Helix-Loop-Helix and Oct Transcription Factor Binding Motifs. FUNCTIONAL ANALYSIS OF THE E{micro}3' ENHANCER OF THE CATFISH. J. Biol. Chem. 276: 27825-27830 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»