This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haviv, I.
Right arrow Articles by Shaul, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haviv, I.
Right arrow Articles by Shaul, Y.

 Previous Article  |  Next Article 

Mol Cell Biol, March 1998, p. 1562-1569, Vol. 18, No. 3
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Hepatitis B Virus pX Targets TFIIB in Transcription Coactivation

Izhak Haviv, Meir Shamay, Gilad Doitsh, and Yosef Shaul*

Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel

Received 30 June 1997/Returned for modification 29 August 1997/Accepted 26 November 1997

pX, the hepatitis B virus (HBV)-encoded regulator, coactivates transcription through an unknown mechanism. pX interacts with several components of the transcription machinery, including certain activators, TFIIB, TFIIH, and the RNA polymerase II (POLII) enzyme. We show that pX localizes in the nucleus and coimmunoprecipitates with TFIIB from nuclear extracts. We used TFIIB mutants inactive in binding either POLII or TATA binding protein to study the role of TFIIB-pX interaction in transcription coactivation. pX was able to bind the former type of TFIIB mutant and not the latter. Neither of these sets of TFIIB mutants supports transcription. Remarkably, the latter TFIIB mutants fully block pX activity, suggesting the role of TFIIB in pX-mediated coactivation. By contrast, in the presence of pX, TFIIB mutants with disrupted POLII binding acquire the wild-type phenotype, both in vivo and in vitro. These results suggest that pX may establish the otherwise inefficient TFIIB mutant-POLII interaction, by acting as a molecular bridge. Collectively, our results demonstrate that TFIIB is the in vivo target of pX.

* Corresponding author. Mailing address: Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel. Phone: 972-8-9342320. Fax: 972-8 9344108. E-mail: lvshaul{at}weizmann.weizmann.ac.il.




This article has been cited by other articles:

  • Fukuda, A., Nakadai, T., Shimada, M., Hisatake, K. (2009). Heterogeneous Nuclear Ribonucleoprotein R Enhances Transcription from the Naturally Configured c-fos Promoter in Vitro. J. Biol. Chem. 284: 23472-23480 [Abstract] [Full Text]  
  • Jung, J. K., Arora, P., Pagano, J. S., Jang, K. L. (2007). Expression of DNA Methyltransferase 1 Is Activated by Hepatitis B Virus X Protein via a Regulatory Circuit Involving the p16INK4a-Cyclin D1-CDK 4/6-pRb-E2F1 Pathway. Cancer Res. 67: 5771-5778 [Abstract] [Full Text]  
  • Li, H., Chi, C.-Y., Lee, S., Andrisani, O. M. (2006). The Mitogenic Function of Hepatitis B Virus X Protein Resides within Amino Acids 51 to 140 and Is Modulated by N- and C-Terminal Regulatory Regions. J. Virol. 80: 10554-10564 [Abstract] [Full Text]  
  • Han, J., Ding, L., Yuan, B., Yang, X., Wang, X., Li, J., Lu, Q., Huang, C., Ye, Q. (2006). Hepatitis B virus X protein and the estrogen receptor variant lacking exon 5 inhibit estrogen receptor signaling in hepatoma cells. Nucleic Acids Res 34: 3095-3106 [Abstract] [Full Text]  
  • Bouchard, M. J., Schneider, R. J. (2004). The Enigmatic X Gene of Hepatitis B Virus. J. Virol. 78: 12725-12734 [Full Text]  
  • Kang, S.-K., Chung, T.-W., Lee, J.-Y., Lee, Y.-C., Morton, R. E., Kim, C.-H. (2004). The Hepatitis B Virus X Protein Inhibits Secretion of Apolipoprotein B by Enhancing the Expression of N-Acetylglucosaminyltransferase III. J. Biol. Chem. 279: 28106-28112 [Abstract] [Full Text]  
  • Chung, T.-W., Lee, Y.-C., Ko, J.-H., Kim, C.-H. (2003). Hepatitis B Virus X Protein Modulates the Expression of PTEN by Inhibiting the Function of p53, a Transcriptional Activator in Liver Cells. Cancer Res. 63: 3453-3458 [Abstract] [Full Text]  
  • Chen, S.-Y., Kao, C.-F., Chen, C.-M., Shih, C.-M., Hsu, M.-J., Chao, C.-H., Wang, S.-H., You, L.-R., Lee, Y.-H. W. (2003). Mechanisms for Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatitis C Virus Core Protein. J. Biol. Chem. 278: 591-607 [Abstract] [Full Text]  
  • Lee, D. K., Kim, B.-C., Kim, I. Y., Cho, E.-a., Satterwhite, D. J., Kim, S.-J. (2002). The Human Papilloma Virus E7 Oncoprotein Inhibits Transforming Growth Factor-beta Signaling by Blocking Binding of the Smad Complex to Its Target Sequence. J. Biol. Chem. 277: 38557-38564 [Abstract] [Full Text]  
  • Tralhao, J. G., Roudier, J., Morosan, S., Giannini, C., Tu, H., Goulenok, C., Carnot, F., Zavala, F., Joulin, V., Kremsdorf, D., Brechot, C. (2002). Paracrine in vivo inhibitory effects of hepatitis B virus X protein (HBx) on liver cell proliferation: An alternative mechanism of HBx-related pathogenesis. Proc. Natl. Acad. Sci. USA 99: 6991-6996 [Abstract] [Full Text]  
  • Shamay, M., Barak, O., Doitsh, G., Ben-Dor, I., Shaul, Y. (2002). Hepatitis B Virus pX Interacts with HBXAP, a PHD Finger Protein to Coactivate Transcription. J. Biol. Chem. 277: 9982-9988 [Abstract] [Full Text]  
  • Xu, Z., Yen, T. S. B., Wu, L., Madden, C. R., Tan, W., Slagle, B. L., Ou, J.-h. (2002). Enhancement of Hepatitis B Virus Replication by Its X Protein in Transgenic Mice. J. Virol. 76: 2579-2584 [Abstract] [Full Text]  
  • Lee, S., Tarn, C., Wang, W.-H., Chen, S., Hullinger, R. L., Andrisani, O. M. (2002). Hepatitis B Virus X Protein Differentially Regulates Cell Cycle Progression in X-transforming Versus Nontransforming Hepatocyte (AML12) Cell Lines. J. Biol. Chem. 277: 8730-8740 [Abstract] [Full Text]  
  • Waris, G., Huh, K.-W., Siddiqui, A. (2001). Mitochondrially Associated Hepatitis B Virus X Protein Constitutively Activates Transcription Factors STAT-3 and NF-kappa B via Oxidative Stress. Mol. Cell. Biol. 21: 7721-7730 [Abstract] [Full Text]  
  • Jang, H. K., Albrecht, R. A., Buczynski, K. A., Kim, S. K., Derbigny, W. A., O'Callaghan, D. J. (2001). Mapping the Sequences That Mediate Interaction of the Equine Herpesvirus 1 Immediate-Early Protein and Human TFIIB. J. Virol. 75: 10219-10230 [Abstract] [Full Text]  
  • Bouchard, M., Giannakopoulos, S., Wang, E. H., Tanese, N., Schneider, R. J. (2001). Hepatitis B Virus HBx Protein Activation of Cyclin A-Cyclin-Dependent Kinase 2 Complexes and G1 Transit via a Src Kinase Pathway. J. Virol. 75: 4247-4257 [Abstract] [Full Text]  
  • Purcell, N. H., Yu, C., He, D., Xiang, J., Paran, N., DiDonato, J. A., Yamaoka, S., Shaul, Y., Lin, A. (2001). Activation of NF-{kappa}B by hepatitis B virus X protein through an I{kappa}B kinase-independent mechanism. Am. J. Physiol. Gastrointest. Liver Physiol. 280: G669-G677 [Abstract] [Full Text]  
  • Lee, D. K., Park, S. H., Yi, Y., Choi, S.-G., Lee, C., Parks, W. T., Cho, H., de Caestecker, M. P., Shaul, Y., Roberts, A. B., Kim, S.-J. (2001). The hepatitis B virus encoded oncoprotein pX amplifies TGF-{beta} family signaling through direct interaction with Smad4: potential mechanism of hepatitis B virus-induced liver fibrosis. Genes Dev. 15: 455-466 [Abstract] [Full Text]  
  • Chang, S.-F., Netter, H. J., Hildt, E., Schuster, R., Schaefer, S., Hsu, Y.-C., Rang, A., Will, H. (2001). Duck Hepatitis B Virus Expresses a Regulatory HBx-Like Protein from a Hidden Open Reading Frame. J. Virol. 75: 161-170 [Abstract] [Full Text]  
  • Seeger, C., Mason, W. S. (2000). Hepatitis B Virus Biology. Microbiol. Mol. Biol. Rev. 64: 51-68 [Abstract] [Full Text]  
  • Paran, N., Ori, A., Haviv, I., Shaul, Y. (2000). A Composite Polyadenylation Signal with TATA Box Function. Mol. Cell. Biol. 20: 834-841 [Abstract] [Full Text]  
  • Barnabas, S., Andrisani, O. M. (2000). Different Regions of Hepatitis B Virus X Protein Are Required for Enhancement of bZip-Mediated Transactivation versus Transrepression. J. Virol. 74: 83-90 [Abstract] [Full Text]  
  • Weil, R., Sirma, H., Giannini, C., Kremsdorf, D., Bessia, C., Dargemont, C., Brechot, C., Israel, A. (1999). Direct Association and Nuclear Import of the Hepatitis B Virus X Protein with the NF-kappa B Inhibitor Ikappa Balpha. Mol. Cell. Biol. 19: 6345-6354 [Abstract] [Full Text]  
  • Tarn, C., Bilodeau, M. L., Hullinger, R. L., Andrisani, O. M. (1999). Differential Immediate Early Gene Expression in Conditional Hepatitis B Virus pX-transforming Versus Nontransforming Hepatocyte Cell Lines. J. Biol. Chem. 274: 2327-2336 [Abstract] [Full Text]  
  • Kershnar, E., Wu, S.-Y., Chiang, C. M. (1998). Immunoaffinity Purification and Functional Characterization of Human Transcription Factor IIH and RNA Polymerase II from Clonal Cell Lines That Conditionally Express Epitope-tagged Subunits of the Multiprotein Complexes. J. Biol. Chem. 273: 34444-34453 [Abstract] [Full Text]  
  • Wang, H.-D., Trivedi, A., Johnson, D. L. (1998). Regulation of RNA Polymerase I-Dependent Promoters by the Hepatitis B Virus X Protein via Activated Ras and TATA-Binding Protein. Mol. Cell. Biol. 18: 7086-7094 [Abstract] [Full Text]  
  • Dorjsuren, D., Lin, Y., Wei, W., Yamashita, T., Nomura, T., Hayashi, N., Murakami, S. (1998). RMP, a Novel RNA Polymerase II Subunit 5-Interacting Protein, Counteracts Transactivation by Hepatitis B Virus X Protein. Mol. Cell. Biol. 18: 7546-7555 [Abstract] [Full Text]  
  • Lin, Y., Tang, H., Nomura, T., Dorjsuren, D., Hayashi, N., Wei, W., Ohta, T., Roeder, R., Murakami, S. (1998). The Hepatitis B Virus X Protein Is a Co-activator of Activated Transcription That Modulates the Transcription Machinery and Distal Binding Activators. J. Biol. Chem. 273: 27097-27103 [Abstract] [Full Text]  
  • Shih, W.-L., Kuo, M.-L., Chuang, S.-E., Cheng, A.-L., Doong, S.-L. (2000). Hepatitis B Virus X Protein Inhibits Transforming Growth Factor-beta -induced Apoptosis through the Activation of Phosphatidylinositol 3-Kinase Pathway. J. Biol. Chem. 275: 25858-25864 [Abstract] [Full Text]  
  • Jaitovich-Groisman, I., Benlimame, N., Slagle, B. L., Perez, M. H., Alpert, L., Song, D. J., Fotouhi-Ardakani, N., Galipeau, J., Alaoui-Jamali, M. A. (2001). Transcriptional Regulation of the TFIIH Transcription Repair Components XPB and XPD by the Hepatitis B Virus x Protein in Liver Cells and Transgenic Liver Tissue. J. Biol. Chem. 276: 14124-14132 [Abstract] [Full Text]  
  • Gomez-Gonzalo, M., Carretero, M., Rullas, J., Lara-Pezzi, E., Aramburu, J., Berkhout, B., Alcami, J., Lopez-Cabrera, M. (2001). The Hepatitis B Virus X Protein Induces HIV-1 Replication and Transcription in Synergy with T-cell Activation Signals. FUNCTIONAL ROLES OF NF-kappa B/NF-AT AND SP1-BINDING SITES IN THE HIV-1 LONG TERMINAL REPEAT PROMOTER. J. Biol. Chem. 276: 35435-35443 [Abstract] [Full Text]  
  • Tarn, C., Lee, S., Hu, Y., Ashendel, C., Andrisani, O. M. (2001). Hepatitis B Virus X Protein Differentially Activates RAS-RAF-MAPK and JNK Pathways in X-transforming Versus Non-transforming AML12 Hepatocytes. J. Biol. Chem. 276: 34671-34680 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»