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Mol Cell Biol, April 1998, p. 1835-1843, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multiple Developmental Requirements of Noisette, the Drosophila Homolog of the U2 snRNP-Associated Polypeptide SF3a⁶⁰

Valérie Meyer, Brian Oliver, and Daniel Pauli^*

Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland

Received 8 September 1997/Returned for modification 19 November 1997/Accepted 20 January 1998

We report the cloning of the noisette gene (noi), which encodes the Drosophila melanogaster ortholog of a U2 snRNP-associated splicing factor, SF3a⁶⁰ (SAP61) in humans and PRP9p in Saccharomyces cerevisiae. Antibodies raised against human SF3a⁶⁰ recognized NOI in flies, showing a nuclear localization in all the stages examined, including the embryo, the dividing cells of imaginal discs, and the larval polyploid nuclei. NOI is expressed in somatic and germinal cells of both male and female gonads. By mobilization of P transposons, we have generated a large number of noi mutations. Complete loss of function resulted in lethality at the end of embryogenesis, without obvious morphological defects. Hypomorphic alleles revealed multiple roles of noi for the survival and differentiation of male germ cells, the differentiation of female germ cells, and the development of several adult structures.

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^* Corresponding author. Mailing address: Department of Zoology and Animal Biology, University of Geneva, 30 Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland. Phone: (41 22) 702 6346. Fax: (41 22) 702 6439. E-mail: pauli{at}sc2a.unige.ch.

Present address: Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892.

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