Previous Article | Next Article 
Mol Cell Biol, April 1998, p. 1879-1890, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Identification and Analysis of Mot3, a Zinc Finger
Protein That Binds to the Retrotransposon Ty Long Terminal Repeat (
)
in Saccharomyces cerevisiae
Jon M.
Madison,
Aimée M.
Dudley, and
Fred
Winston*
Department of Genetics, Harvard Medical
School, Boston, Massachusetts 02115
Received 2 October 1997/Returned for modification 13 November
1997/Accepted 6 January 1998
Spt3 and Mot1 are two transcription factors of Saccharomyces
cerevisiae that are thought to act in a related fashion to
control the function of TATA-binding protein (TBP). Current models
suggest that while Spt3 and Mot1 do not directly interact, they do
function in a related fashion to stabilize the TBP-TATA interaction at particular promoters. Consistent with this model, certain combinations of spt3 and mot1 mutations are inviable. To
identify additional proteins related to Spt3 and Mot1 functions, we
screened for high-copy-number suppressors of the mot1 spt3
inviability. This screen identified a previously unstudied gene,
MOT3, that encodes a zinc finger protein. We show that Mot3
binds in vitro to three sites within the retrotransposon Ty long
terminal repeat (
) sequence. One of these sites is immediately 5' of
the
TATA region. Although a mot3 null mutation causes
no strong phenotypes, it does cause some mild phenotypes, including a
very modest increase in Ty mRNA levels, partial suppression of
transcriptional defects caused by a mot1 mutation, and
partial suppression of an spt3 mutation. These results, in
conjunction with those of an independent study of Mot3 (A. Grishin, M. Rothenberg, M. A. Downs, and K. J. Blumer, Genetics, in
press), suggest that this protein plays a varied role in gene
expression that may be largely redundant with other factors.
*
Corresponding author. Mailing address: Department of
Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-3993. E-mail:
winston{at}rascal.med.harvard.edu.
This article has been cited by other articles:
-
Servant, G., Pennetier, C., Lesage, P.
(2008). Remodeling Yeast Gene Transcription by Activating the Ty1 Long Terminal Repeat Retrotransposon under Severe Adenine Deficiency. Mol. Cell. Biol.
28: 5543-5554
[Abstract]
[Full Text]
-
Sertil, O., Vemula, A., Salmon, S. L., Morse, R. H., Lowry, C. V.
(2007). Direct Role for the Rpd3 Complex in Transcriptional Induction of the Anaerobic DAN/TIR Genes in Yeast. Mol. Cell. Biol.
27: 2037-2047
[Abstract]
[Full Text]
-
Todeschini, A.-L., Morillon, A., Springer, M., Lesage, P.
(2005). Severe Adenine Starvation Activates Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae. Mol. Cell. Biol.
25: 7459-7472
[Abstract]
[Full Text]
-
Klinkenberg, L. G., Mennella, T. A., Luetkenhaus, K., Zitomer, R. S.
(2005). Combinatorial Repression of the Hypoxic Genes of Saccharomyces cerevisiae by DNA Binding Proteins Rox1 and Mot3. Eukaryot Cell
4: 649-660
[Abstract]
[Full Text]
-
Bachman, N., Eby, Y., Boeke, J. D.
(2004). Local Definition of Ty1 Target Preference by Long Terminal Repeats and Clustered tRNA Genes. Genome Res
14: 1232-1247
[Abstract]
[Full Text]
-
Sertil, O., Kapoor, R., Cohen, B. D., Abramova, N., Lowry, C. V.
(2003). Synergistic repression of anaerobic genes by Mot3 and Rox1 in Saccharomyces cerevisiae. Nucleic Acids Res
31: 5831-5837
[Abstract]
[Full Text]
-
Morillon, A., Benard, L., Springer, M., Lesage, P.
(2002). Differential Effects of Chromatin and Gcn4 on the 50-Fold Range of Expression among Individual Yeast Ty1 Retrotransposons. Mol. Cell. Biol.
22: 2078-2088
[Abstract]
[Full Text]
-
Abramova, N. E., Cohen, B. D., Sertil, O., Kapoor, R., Davies, K. J. A., Lowry, C. V.
(2001). Regulatory Mechanisms Controlling Expression of the DAN/TIR Mannoprotein Genes During Anaerobic Remodeling of the Cell Wall in Saccharomyces cerevisiae. Genetics
157: 1169-1177
[Abstract]
[Full Text]
-
Cohen, B. D., Sertil, O., Abramova, N. E., Davies, K. J. A., Lowry, C. V.
(2001). Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites. Nucleic Acids Res
29: 799-808
[Abstract]
[Full Text]
-
Kastaniotis, A. J., Mennella, T. A., Konrad, C., Torres, A. M. R., Zitomer, R. S.
(2000). Roles of Transcription Factor Mot3 and Chromatin in Repression of the Hypoxic Gene ANB1 in Yeast. Mol. Cell. Biol.
20: 7088-7098
[Abstract]
[Full Text]
-
Dudley, A. M., Gansheroff, L. J., Winston, F.
(1999). Specific Components of the SAGA Complex Are Required for Gcn4- and Gcr1-Mediated Activation of the his4-912{delta} Promoter in Saccharomyces cerevisiae. Genetics
151: 1365-1378
[Abstract]
[Full Text]
-
Grishin, A. V., Rothenberg, M., Downs, M. A., Blumer, K. J.
(1998). Mot3, a Zn Finger Transcription Factor That Modulates Gene Expression and Attenuates Mating Pheromone Signaling in Saccharomyces cerevisiae. Genetics
149: 879-892
[Abstract]
[Full Text]