This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sidote, D.
Right arrow Articles by Edery, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sidote, D.
Right arrow Articles by Edery, I.

 Previous Article  |  Next Article 

Mol Cell Biol, April 1998, p. 2004-2013, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differential Effects of Light and Heat on the Drosophila Circadian Clock Proteins PER and TIM

David Sidote,1 John Majercak,2 Vaishali Parikh,3,dagger and Isaac Edery1,*

Department of Molecular Biology and Biochemistry1 and Biochemistry Graduate Program,2 Center for Advanced Biotechnology and Medicine, Rutgers University, and Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey,3 Piscataway, New Jersey 08854

Received 21 August 1997/Returned for modification 1 October 1997/Accepted 6 January 1998

Circadian (congruent 24-h) rhythms are governed by endogenous biochemical oscillators (clocks) that in a wide variety of organisms can be phase shifted (i.e., delayed or advanced) by brief exposure to light and changes in temperature. However, how changes in temperature reset circadian timekeeping mechanisms is not known. To begin to address this issue, we measured the effects of short-duration heat pulses on the protein and mRNA products from the Drosophila circadian clock genes period (per) and timeless (tim). Heat pulses at all times in a daily cycle elicited dramatic and rapid decreases in the levels of PER and TIM proteins. PER is sensitive to heat but not light, indicating that individual clock components can markedly differ in sensitivity to environmental stimuli. A similar resetting mechanism involving delays in the per-tim transcriptional-translational feedback loop likely underlies the observation that when heat and light signals are administered in the early night, they both evoke phase delays in behavioral rhythms. However, whereas previous studies showed that the light-induced degradation of TIM in the late night is accompanied by stable phase advances in the temporal regulation of the PER and TIM biochemical rhythms, the heat-induced degradation of PER and TIM at these times in a daily cycle results in little, if any, long-term perturbation in the cycles of these clock proteins. Rather, the initial heat-induced degradation of PER and TIM in the late night is followed by a transient and rapid increase in the speed of the PER-TIM temporal program. The net effect of these heat-induced changes results in an oscillatory mechanism with a steady-state phase similar to that of the unperturbed control situation. These findings can account for the lack of apparent steady-state shifts in Drosophila behavioral rhythms by heat pulses applied in the late night and strongly suggest that stimulus-induced changes in the speed of circadian clocks can contribute to phase-shifting responses.

* Corresponding author. Mailing address: Department of Molecular Biology and Biochemistry, CABM, 679 Hoes Lane, Piscataway, NJ 08854. Phone: (732) 235-5550. Fax: (732) 235-5318. E-mail: edery{at}mbcl.rutgers.edu.

dagger Present address: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.




This article has been cited by other articles:

  • Picot, M., Klarsfeld, A., Chelot, E., Malpel, S., Rouyer, F. (2009). A Role for Blind DN2 Clock Neurons in Temperature Entrainment of the Drosophila Larval Brain. J. Neurosci. 29: 8312-8320 [Abstract] [Full Text]  
  • Yu, W., Zheng, H., Price, J. L., Hardin, P. E. (2009). DOUBLETIME Plays a Noncatalytic Role To Mediate CLOCK Phosphorylation and Repress CLOCK-Dependent Transcription within the Drosophila Circadian Clock. Mol. Cell. Biol. 29: 1452-1458 [Abstract] [Full Text]  
  • Yoshii, T., Wulbeck, C., Sehadova, H., Veleri, S., Bichler, D., Stanewsky, R., Helfrich-Forster, C. (2009). The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase of Drosophila's Clock. J. Neurosci. 29: 2597-2610 [Abstract] [Full Text]  
  • Fan, J.-Y., Muskus, M. J., Price, J. L. (2007). Entrainment of the Drosophila Circadian Clock: More Heat Than Light. Sci Signal 2007: pe65-pe65 [Abstract] [Full Text]  
  • Lim, C., Lee, J., Choi, C., Kim, J., Doh, E., Choe, J. (2007). Functional Role of CREB-Binding Protein in the Circadian Clock System of Drosophila melanogaster. Mol. Cell. Biol. 27: 4876-4890 [Abstract] [Full Text]  
  • Kim, E. Y., Ko, H. W., Yu, W., Hardin, P. E., Edery, I. (2007). A DOUBLETIME Kinase Binding Domain on the Drosophila PERIOD Protein Is Essential for Its Hyperphosphorylation, Transcriptional Repression, and Circadian Clock Function. Mol. Cell. Biol. 27: 5014-5028 [Abstract] [Full Text]  
  • Glaser, F. T., Stanewsky, R. (2007). Synchronization of the Drosophila Circadian Clock by Temperature Cycles. Cold Spring Harb Symp Quant Biol 72: 233-242 [Abstract]  
  • Chen, W.-F., Low, K. H., Lim, C., Edery, I. (2007). Thermosensitive Splicing of a Clock Gene and Seasonal Adaptation. Cold Spring Harb Symp Quant Biol 72: 599-606 [Abstract]  
  • Peschel, N., Veleri, S., Stanewsky, R. (2006). Veela defines a molecular link between Cryptochrome and Timeless in the light-input pathway to Drosophila's circadian clock. Proc. Natl. Acad. Sci. USA 103: 17313-17318 [Abstract] [Full Text]  
  • Chen, W.-F., Majercak, J., Edery, I. (2006). Clock-Gated Photic Stimulation of Timeless Expression at Cold Temperatures and Seasonal Adaptation in Drosophila. J Biol Rhythms 21: 256-271 [Abstract]  
  • Hong, S.-T., Bang, S., Paik, D., Kang, J., Hwang, S., Jeon, K., Chun, B., Hyun, S., Lee, Y., Kim, J. (2006). Histamine and its receptors modulate temperature-preference behaviors in Drosophila.. J. Neurosci. 26: 7245-7256 [Abstract] [Full Text]  
  • Preuss, F., Fan, J.-Y., Kalive, M., Bao, S., Schuenemann, E., Bjes, E. S., Price, J. L. (2004). Drosophila doubletime Mutations Which either Shorten or Lengthen the Period of Circadian Rhythms Decrease the Protein Kinase Activity of Casein Kinase I. Mol. Cell. Biol. 24: 886-898 [Abstract] [Full Text]  
  • Ashmore, L. J., Sehgal, A. (2003). A Fly's Eye View of Circadian Entrainment. J Biol Rhythms 18: 206-216 [Abstract]  
  • Hendricks, J. C. (2003). Genetic Models in Applied Physiology: Invited Review: Sleeping flies don't lie: the use of Drosophila melanogaster to study sleep and circadian rhythms. J. Appl. Physiol. 94: 1660-1672 [Abstract] [Full Text]  
  • Damiola, F., Le Minh, N., Preitner, N., Kornmann, B., Fleury-Olela, F., Schibler, U. (2000). Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus. Genes Dev. 14: 2950-2961 [Abstract] [Full Text]  
  • Bae, K., Lee, C., Hardin, P. E., Edery, I. (2000). dCLOCK Is Present in Limiting Amounts and Likely Mediates Daily Interactions between the dCLOCK-CYC Transcription Factor and the PER-TIM Complex. J. Neurosci. 20: 1746-1753 [Abstract] [Full Text]  
  • Lee, C., Bae, K., Edery, I. (1999). PER and TIM Inhibit the DNA Binding Activity of a Drosophila CLOCK-CYC/dBMAL1 Heterodimer without Disrupting Formation of the Heterodimer: a Basis for Circadian Transcription. Mol. Cell. Biol. 19: 5316-5325 [Abstract] [Full Text]  
  • Taylor, B. L., Zhulin, I. B. (1999). PAS Domains: Internal Sensors of Oxygen, Redox Potential, and Light. Microbiol. Mol. Biol. Rev. 63: 479-506 [Abstract] [Full Text]  
  • Egan, E. S., Franklin, T. M., Hilderbrand-Chae, M. J., McNeil, G. P., Roberts, M. A., Schroeder, A. J., Zhang, X., Jackson, F. R. (1999). An Extraretinally Expressed Insect Cryptochrome with Similarity to the Blue Light Photoreceptors of Mammals and Plants. J. Neurosci. 19: 3665-3673 [Abstract] [Full Text]  
  • Bae, K., Lee, C., Sidote, D., Chuang, K.-y., Edery, I. (1998). Circadian Regulation of a Drosophila Homolog of the Mammalian Clock Gene: PER and TIM Function as Positive Regulators. Mol. Cell. Biol. 18: 6142-6151 [Abstract] [Full Text]  
  • Liu, Y., Merrow, M., Loros, J. J., Dunlap, J. C. (1998). How Temperature Changes Reset a Circadian Oscillator. Science 281: 825-829 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»