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Mol Cell Biol, April 1998, p. 2045-2054, Vol. 18, No. 4
Rosenstiel Center and Department of Biology,
Brandeis University, Waltham, Massachusetts 02254-9110
Received 25 November 1997/Accepted 16 January 1998
Repair of a double-strand break (DSB) in yeast can induce very
frequent expansions and contractions in a tandem array of 375-bp repeats. These results strongly suggest that DSB repair can be a major
source of amplification of tandemly repeated sequences. Most of the DSB
repair events are not associated with crossover. Rearrangements appear
in 50% of these repaired recipient molecules. In contrast, the donor
template nearly always remains unchanged. Among the rare crossover
events, similar rearrangements are found. These results cannot readily
be explained by the gap repair model of Szostak et al. (J. W. Szostak, T. L. Orr-Weaver, R. J. Rothstein, and F. W. Stahl, Cell 33:25-35, 1983) but can be explained by synthesis-dependent strand annealing (SDSA) models that allow for
crossover. Support for SDSA models is provided by a demonstration that
a single DSB repair event can use two donor templates located on two
different chromosomes.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Expansions and Contractions in a Tandem Repeat
Induced by Double-Strand Break Repair
*
Corresponding author. Mailing address: Rosenstiel
Center, Brandeis University, Waltham, MA 02254-9110. Phone: (781)
736-2462. Fax: (781) 736-2405. E-mail:
haber{at}hydra.rose.brandeis.edu.
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