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Mol Cell Biol, April 1998, p. 2045-2054, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expansions and Contractions in a Tandem Repeat Induced by Double-Strand Break Repair

Frédéric Pâques, Wai-Ying Leung, and James E. Haber*

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110

Received 25 November 1997/Accepted 16 January 1998

Repair of a double-strand break (DSB) in yeast can induce very frequent expansions and contractions in a tandem array of 375-bp repeats. These results strongly suggest that DSB repair can be a major source of amplification of tandemly repeated sequences. Most of the DSB repair events are not associated with crossover. Rearrangements appear in 50% of these repaired recipient molecules. In contrast, the donor template nearly always remains unchanged. Among the rare crossover events, similar rearrangements are found. These results cannot readily be explained by the gap repair model of Szostak et al. (J. W. Szostak, T. L. Orr-Weaver, R. J. Rothstein, and F. W. Stahl, Cell 33:25-35, 1983) but can be explained by synthesis-dependent strand annealing (SDSA) models that allow for crossover. Support for SDSA models is provided by a demonstration that a single DSB repair event can use two donor templates located on two different chromosomes.

* Corresponding author. Mailing address: Rosenstiel Center, Brandeis University, Waltham, MA 02254-9110. Phone: (781) 736-2462. Fax: (781) 736-2405. E-mail: haber{at}hydra.rose.brandeis.edu.




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