This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Scharf, K.-D. Articles by Nover, L. Search for Related Content PubMed PubMed Citation Articles by Scharf, K.-D. Articles by Nover, L.

Mol Cell Biol, April 1998, p. 2240-2251, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Tomato Hsf System: HsfA2 Needs Interaction with HsfA1 for Efficient Nuclear Import and May Be Localized in Cytoplasmic Heat Stress Granules

Klaus-Dieter Scharf, Harald Heider, Ingo Höhfeld, Ruth Lyck, Enrico Schmidt, and Lutz Nover^*

Department of Molecular Cell Biology, Goethe University Frankfurt, D-60439 Frankfurt/Main, Germany

Received 31 October 1997/Returned for modification 16 December 1997/Accepted 12 January 1998

In heat-stressed (HS) tomato (Lycopersicon peruvianum) cell cultures, the constitutively expressed HS transcription factor HsfA1 is complemented by two HS-inducible forms, HsfA2 and HsfB1. Because of its stability, HsfA2 accumulates to fairly high levels in the course of a prolonged HS and recovery regimen. Using immunofluorescence and cell fractionation experiments, we identified three states of HsfA2: (i) a soluble, cytoplasmic form in preinduced cultures maintained at 25°C, (ii) a salt-resistant, nuclear form found in HS cells, and (iii) a stored form of HsfA2 in cytoplasmic HS granules. The efficient nuclear transport of HsfA2 evidently requires interaction with HsfA1. When expressed in tobacco protoplasts by use of a transient-expression system, HsfA2 is mainly retained in the cytoplasm unless it is coexpressed with HsfA1. The essential parts for the interaction and nuclear cotransport of the two Hsfs are the homologous oligomerization domain (HR-A/B region of the A-type Hsfs) and functional nuclear localization signal motifs of both partners. Direct physical interaction of the two Hsfs with formation of relatively stabile hetero-oligomers was shown by a two-hybrid test in Saccharomyces cerevisiae as well as by coimmunoprecipitation using tomato and tobacco whole-cell lysates.

---

^* Corresponding author. Mailing address: Department of Molecular Cell Biology, Biocenter N200, 30G, Goethe University Frankfurt, Marie Curie Str. 9, D-60439 Frankfurt/Main, Germany. Phone: 49- 69-798-29284. Fax: 49-69-798-29286. E-mail: nover{at}cellbiology.uni-frankfurt.de.

Present address: DIBIT-Scientific Institute San Raffaele, I-20132 Milan, Italy.

This article has been cited by other articles:

• Snyman, M., Cronje, M. J. (2008). Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings. J Exp Bot 0: ern075v1-ern075 [Abstract] [Full Text]  
• Kim, J.-E., Ryu, I., Kim, W. J., Song, O.-K., Ryu, J., Kwon, M. Y., Kim, J. H., Jang, S. K. (2008). Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation. Mol. Cell. Biol. 28: 803-813 [Abstract] [Full Text]  
• Baniwal, S. K., Chan, K. Y., Scharf, K.-D., Nover, L. (2007). Role of Heat Stress Transcription Factor HsfA5 as Specific Repressor of HsfA4. J. Biol. Chem. 282: 3605-3613 [Abstract] [Full Text]  
• Kotak, S., Vierling, E., Baumlein, H., Koskull-Doring, P. v. (2007). A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis. Plant Cell 19: 182-195 [Abstract] [Full Text]  
• Charng, Y.-y., Liu, H.-c., Liu, N.-y., Chi, W.-t., Wang, C.-n., Chang, S.-h., Wang, T.-t. (2007). A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in Arabidopsis. Plant Physiol. 143: 251-262 [Abstract] [Full Text]  
• Jolly, C., Lakhotia, S. C. (2006). Human sat III and Drosophila hsr{omega} transcripts: a common paradigm for regulation of nuclear RNA processing in stressed cells. Nucleic Acids Res 34: 5508-5514 [Abstract] [Full Text]  
• MILLER, G., MITTLER, R. (2006). Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?. ANN BOT (LOND) 98: 279-288 [Abstract] [Full Text]  
• Kim, W. J., Back, S. H., Kim, V., Ryu, I., Jang, S. K. (2005). Sequestration of TRAF2 into Stress Granules Interrupts Tumor Necrosis Factor Signaling under Stress Conditions. Mol. Cell. Biol. 25: 2450-2462 [Abstract] [Full Text]  
• Port, M., Tripp, J., Zielinski, D., Weber, C., Heerklotz, D., Winkelhaus, S., Bublak, D., Scharf, K.-D. (2004). Role of Hsp17.4-CII as Coregulator and Cytoplasmic Retention Factor of Tomato Heat Stress Transcription Factor HsfA2. Plant Physiol. 135: 1457-1470 [Abstract] [Full Text]  
• Bharti, K., von Koskull-Doring, P., Bharti, S., Kumar, P., Tintschl-Korbitzer, A., Treuter, E., Nover, L. (2004). Tomato Heat Stress Transcription Factor HsfB1 Represents a Novel Type of General Transcription Coactivator with a Histone-Like Motif Interacting with the Plant CREB Binding Protein Ortholog HAC1. Plant Cell 16: 1521-1535 [Abstract] [Full Text]  
• Anderson, P., Kedersha, N. (2002). Stressful initiations. J. Cell Sci. 115: 3227-3234 [Abstract] [Full Text]  
• Rojas, A., Almoguera, C., Carranco, R., Scharf, K.-D., Jordano, J. (2002). Selective Activation of the Developmentally Regulated Ha hsp17.6 G1 Promoter by Heat Stress Transcription Factors. Plant Physiol. 129: 1207-1215 [Abstract] [Full Text]  
• Mishra, S. K., Tripp, J., Winkelhaus, S., Tschiersch, B., Theres, K., Nover, L., Scharf, K.-D. (2002). In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes Dev. 16: 1555-1567 [Abstract] [Full Text]  
• Narberhaus, F. (2002). {alpha}-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network. Microbiol. Mol. Biol. Rev. 66: 64-93 [Abstract] [Full Text]  
• Mathew, A., Mathur, S. K., Jolly, C., Fox, S. G., Kim, S., Morimoto, R. I. (2001). Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2. Mol. Cell. Biol. 21: 7163-7171 [Abstract] [Full Text]  
• Heerklotz, D., Döring, P., Bonzelius, F., Winkelhaus, S., Nover, L. (2001). The Balance of Nuclear Import and Export Determines the Intracellular Distribution and Function of Tomato Heat Stress Transcription Factor HsfA2. Mol. Cell. Biol. 21: 1759-1768 [Abstract] [Full Text]  
• Kedersha, N., Cho, M. R., Li, W., Yacono, P. W., Chen, S., Gilks, N., Golan, D. E., Anderson, P. (2000). Dynamic Shuttling of TIA-1 Accompanies the Recruitment of mRNA to Mammalian Stress Granules. J. Cell Biol. 151: 1257-1268 [Abstract] [Full Text]  
• Döring, P., Treuter, E., Kistner, C., Lyck, R., Chen, A., Nover, L. (2000). The Role of AHA Motifs in the Activator Function of Tomato Heat Stress Transcription Factors HsfA1 and HsfA2. Plant Cell 12: 265-278 [Abstract] [Full Text]  
• Kedersha, N. L., Gupta, M., Li, W., Miller, I., Anderson, P. (1999). RNA-binding Proteins TIA-1 and TIAR Link the Phosphorylation of eIF-2{alpha} to the Assembly of Mammalian Stress Granules. J. Cell Biol. 147: 1431-1442 [Abstract] [Full Text]  
• Stuger, R., Ranostaj, S., Materna, T., Forreiter, C. (1999). Messenger RNA-Binding Properties of Nonpolysomal Ribonucleoproteins from Heat-Stressed Tomato Cells. Plant Physiol. 120: 23-32 [Abstract] [Full Text]  
• Kedersha, N., Chen, S., Gilks, N., Li, W., Miller, I. J., Stahl, J., Anderson, P. (2002). Evidence That Ternary Complex (eIF2-GTP-tRNAiMet)-Deficient Preinitiation Complexes Are Core Constituents of Mammalian Stress Granules. Mol. Biol. Cell 13: 195-210 [Abstract] [Full Text]