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Mol Cell Biol, April 1998, p. 2252-2261, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Adf-1 Is a Nonmodular Transcription Factor That Contains a TAF-Binding Myb-Like Motif

Gene Cutler,dagger Kathleen M. Perry, and Robert Tjian*

Howard Hughes Medical Institute, University of California, Berkeley, California 94720

Received 28 October 1997/Returned for modification 7 January 1998/Accepted 27 January 1998

Adf-1 is an essential Drosophila melanogaster sequence-specific transactivator that binds the promoters of a diverse group of genes. We have performed a comprehensive mapping of the functional domains of Adf-1 to study the role of transactivators in the process of gene activation. Using a series of clustered point mutations and small deletions we have identified regions of Adf-1 required for DNA binding, dimerization, and activation. In contrast to most enhancer-binding factors, the Adf-1 activation regions are nonmodular and depend on an intact protein, including the Adf-1 DNA-binding domain, for activity. Like many transcriptional activators, Adf-1 contains a TFIID-binding domain that can interact with specific TAF subunits. Although TAFs are required for Adf-1-directed activation, TAF binding is not sufficient, suggesting that Adf-1 may direct multiple essential steps during activation. Interestingly, both the TAF-binding domain and the DNA-binding domain contain sequences homologous to those of the Myb family of DNA-binding domains. Thus, Adf-1 has evolved an unusual structure containing two versions of the Myb motif, one that binds DNA and one that binds proteins.

* Corresponding author. Mailing address: Molecular and Cell Biology, 401 Barker Hall Mailroom, University of California, Berkeley, CA 94720-3204. Phone: (510) 642-0884. Fax: (510) 643-9547. E-mail: jmlim{at}uclink4.berkeley.edu.

dagger Present address: Tulerik Inc., South San Francisco, Calif.




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