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Mol Cell Biol, May 1998, p. 2659-2667, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Increased c-fos mRNA Expression By Human Fibroblasts Contracting Stressed Collagen Matrices

Hans Rosenfeldt, David J. Lee, and Frederick Grinnell*

Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical School, Dallas, Texas 75235-9039

Received 29 August 1997/Returned for modification 27 October 1997/Accepted 17 February 1998

We studied early changes in gene expression during fibroblast contraction of stressed collagen matrices. The level of c-fos mRNA increased dramatically and peaked 50 to 60 min after matrix contraction was initiated. This response did not require serum and could not be accounted for simply by disruption of the actin cytoskeleton. Increased c-fos mRNA levels required Ca2+ influx but not the cyclic AMP or extracellular signal-regulated kinase (ERK 1/2) signaling pathways, both of which are activated when fibroblasts contract stressed collagen matrices. The levels of two other immediate-early genes, fosb and c-jun, also increased transiently after fibroblast contraction, whereas the levels of fra-1, fra-2, c-myc, and the transcription factor NF-kappa B remained the same, indicating that fibroblast contraction caused changes in a selective group of genes. The increase in c-fos mRNA during contraction of stressed collagen matrices may reflect a unique role for c-fos in mechanoregulated events at the end of wound repair.

* Corresponding author. Mailing address: Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical School, Dallas, TX 75235-9039. Phone: (214) 648-2181. Fax: (214) 648-8694. E-mail: grinne01{at}utsw.swmed.edu.

Mol Cell Biol, May 1998, p. 2659-2667, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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