Relationship of the Xeroderma Pigmentosum Group E DNA Repair Defect to the Chromatin and DNA Binding Proteins UV-DDB and Replication Protein A

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rapic Otrin, V.
	Articles by Wood, R. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rapic Otrin, V.
	Articles by Wood, R. D.

Previous Article | Next Article

Mol Cell Biol, June 1998, p. 3182-3190, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Relationship of the Xeroderma Pigmentosum Group E DNA Repair Defect to the Chromatin and DNA Binding Proteins UV-DDB and Replication Protein A

Vesna Rapi $c'$ Otrin,¹ Isao Kuraoka,² Tiziana Nardo,³ Mary McLenigan,¹ A. P. M. Eker,⁴ Miria Stefanini,³ Arthur S. Levine,¹^,^* and Richard D. Wood²

Section on DNA Replication, Repair, and Mutagenesis, National Institute of Child Health and Human Development, Bethesda, Maryland 20892-2725¹; Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom²; Istituto di Genetica Biochimica ed Evolutionisticia, CNR, 27100 Pavia, Italy³; and Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands⁴

Received 23 October 1997/Returned for modification 15 December 1997/Accepted 19 March 1998

Cells from complementation groups A through G of the heritable sun-sensitive disorder xeroderma pigmentosum (XP) show defectsin nucleotide excision repair of damaged DNA. Proteins representinggroups A, B, C, D, F, and G are subunits of the core recognitionand incision machinery of repair. XP group E (XP-E) is the mildestform of the disorder, and cells generally show about 50% of thenormal repair level. We investigated two protein factors previouslyimplicated in the XP-E defect, UV-damaged DNA binding protein(UV-DDB) and replication protein A (RPA). Three newly identifiedXP-E cell lines (XP23PV, XP25PV, and a line formerly classifiedas an XP variant) were defective in UV-DDB binding activity buthad levels of RPA in the normal range. The XP-E cell extractsdid not display a significant nucleotide excision repair defectin vitro, with either UV-irradiated DNA or a uniquely placed cisplatinlesion used as a substrate. Purified UV-DDB protein did not stimulaterepair of naked DNA by DDB XP-E cell extracts, but microinjection of the protein into DDB XP-E cells could partially correct the repair defect. RPA stimulatedrepair in normal, XP-E, or complemented extracts from other XPgroups, and so the effect of RPA was not specific for XP-E cellextracts. These data strengthen the connection between XP-E andUV-DDB. Coupled with previous results, the findings suggest thatUV-DDB has a role in the repair of DNA in chromatin.

^* Corresponding author. Mailing address: Section on DNA Replication, Repair, and Mutagenesis, National Institute of Child Healthand Human Development, NIH, Building 6, Rm. 1A15, Bethesda, MD20892-2725. Phone: (301) 496-2133. Fax: (301) 402-0105. E-mail:LevineA{at}EXCHANGE.NIH.GOV.

Mol Cell Biol, June 1998, p. 3182-3190, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Guerrero-Santoro, J., Kapetanaki, M. G., Hsieh, C. L., Gorbachinsky, I., Levine, A. S., Rapic-Otrin, V. (2008). The Cullin 4B-Based UV-Damaged DNA-Binding Protein Ligase Binds to UV-Damaged Chromatin and Ubiquitinates Histone H2A. Cancer Res. 68: 5014-5022 [Abstract] [Full Text]
Stoyanova, T., Yoon, T., Kopanja, D., Mokyr, M. B., Raychaudhuri, P. (2008). The Xeroderma Pigmentosum Group E Gene Product DDB2 Activates Nucleotide Excision Repair by Regulating the Level of p21Waf1/Cip1. Mol. Cell. Biol. 28: 177-187 [Abstract] [Full Text]
Tan, L., Ehrlich, E., Yu, X.-F. (2007). DDB1 and Cul4A Are Required for Human Immunodeficiency Virus Type 1 Vpr-Induced G2 Arrest. J. Virol. 81: 10822-10830 [Abstract] [Full Text]
Wang, Q.-E., Praetorius-Ibba, M., Zhu, Q., El-Mahdy, M. A., Wani, G., Zhao, Q., Qin, S., Patnaik, S., Wani, A. A. (2007). Ubiquitylation-independent degradation of Xeroderma pigmentosum group C protein is required for efficient nucleotide excision repair. Nucleic Acids Res 35: 5338-5350 [Abstract] [Full Text]
Li, J., Wang, Q.-E., Zhu, Q., El-Mahdy, M. A., Wani, G., Praetorius-Ibba, M., Wani, A. A. (2006). DNA Damage Binding Protein Component DDB1 Participates in Nucleotide Excision Repair through DDB2 DNA-binding and Cullin 4A Ubiquitin Ligase Activity.. Cancer Res. 66: 8590-8597 [Abstract] [Full Text]
Hu, Z., Shao, M., Yuan, J., Xu, L., Wang, F., Wang, Y., Yuan, W., Qian, J., Ma, H., Wang, Y., Liu, H., Chen, W., Yang, L., Jin, G., Huo, X., Chen, F., Jin, L., Wei, Q., Huang, W., Lu, D., Wu, T., Shen, H. (2006). Polymorphisms in DNA damage binding protein 2 (DDB2) and susceptibility of primary lung cancer in the Chinese: a case-control study. Carcinogenesis 27: 1475-1480 [Abstract] [Full Text]
Kapetanaki, M. G., Guerrero-Santoro, J., Bisi, D. C., Hsieh, C. L., Rapic-Otrin, V., Levine, A. S. (2006). The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites. Proc. Natl. Acad. Sci. USA 103: 2588-2593 [Abstract] [Full Text]
Shimanouchi, K., Takata, K.-i., Yamaguchi, M., Murakami, S., Ishikawa, G., Takeuchi, R., Kanai, Y., Ruike, T., Nakamura, R., Abe, Y., Sakaguchi, K. (2006). Drosophila Damaged DNA Binding Protein 1 Contributes to Genome Stability in Somatic Cells. J Biochem 139: 51-58 [Abstract] [Full Text]
Kulaksiz, G., Reardon, J. T., Sancar, A. (2005). Xeroderma Pigmentosum Complementation Group E Protein (XPE/DDB2): Purification of Various Complexes of XPE and Analyses of Their Damaged DNA Binding and Putative DNA Repair Properties. Mol. Cell. Biol. 25: 9784-9792 [Abstract] [Full Text]
Alekseev, S., Kool, H., Rebel, H., Fousteri, M., Moser, J., Backendorf, C., de Gruijl, F. R., Vrieling, H., Mullenders, L. H.F. (2005). Enhanced DDB2 Expression Protects Mice from Carcinogenic Effects of Chronic UV-B Irradiation. Cancer Res. 65: 10298-10306 [Abstract] [Full Text]
Wang, Q.-E., Zhu, Q., Wani, G., Chen, J., Wani, A. A. (2004). UV radiation-induced XPC translocation within chromatin is mediated by damaged-DNA binding protein, DDB2. Carcinogenesis 25: 1033-1043 [Abstract] [Full Text]
Bondar, T., Ponomarev, A., Raychaudhuri, P. (2004). Ddb1 Is Required for the Proteolysis of the Schizosaccharomyces pombe Replication Inhibitor Spd1 during S Phase and after DNA Damage. J. Biol. Chem. 279: 9937-9943 [Abstract] [Full Text]
Itoh, T., Cado, D., Kamide, R., Linn, S. (2004). DDB2 gene disruption leads to skin tumors and resistance to apoptosis after exposure to ultraviolet light but not a chemical carcinogen. Proc. Natl. Acad. Sci. USA 101: 2052-2057 [Abstract] [Full Text]
Reardon, J. T., Sancar, A. (2003). Recognition and repair of the cyclobutane thymine dimer, a major cause of skin cancers, by the human excision nuclease. Genes Dev. 17: 2539-2551 [Abstract] [Full Text]
Bondar, T., Mirkin, E. V., Ucker, D. S., Walden, W. E., Mirkin, S. M., Raychaudhuri, P. (2003). Schizosaccharomyces pombe Ddb1 Is functionally Linked to the Replication Checkpoint Pathway. J. Biol. Chem. 278: 37006-37014 [Abstract] [Full Text]
Rapic-Otrin, V., Navazza, V., Nardo, T., Botta, E., McLenigan, M., Bisi, D. C., Levine, A. S., Stefanini, M. (2003). True XP group E patients have a defective UV-damaged DNA binding protein complex and mutations in DDB2 which reveal the functional domains of its p48 product. Hum Mol Genet 12: 1507-1522 [Abstract] [Full Text]
Leupin, O., Bontron, S., Strubin, M. (2003). Hepatitis B Virus X Protein and Simian Virus 5 V Protein Exhibit Similar UV-DDB1 Binding Properties To Mediate Distinct Activities. J. Virol. 77: 6274-6283 [Abstract] [Full Text]
Fitch, M. E., Cross, I. V., Ford, J. M. (2003). p53 responsive nucleotide excision repair gene products p48 and XPC, but not p53, localize to sites of UV-irradiation-induced DNA damage, in vivo. Carcinogenesis 24: 843-850 [Abstract] [Full Text]
Nichols, A. F., Itoh, T., Zolezzi, F., Hutsell, S., Linn, S. (2003). Basal transcriptional regulation of human damage-specific DNA-binding protein genes DDB1 and DDB2 by Sp1, E2F, N-myc and NF1 elements. Nucleic Acids Res 31: 562-569 [Abstract] [Full Text]
Bontron, S., Lin-Marq, N., Strubin, M. (2002). Hepatitis B Virus X Protein Associated with UV-DDB1 Induces Cell Death in the Nucleus and Is Functionally Antagonized by UV-DDB2. J. Biol. Chem. 277: 38847-38854 [Abstract] [Full Text]
Cong, F., Tang, J., Hwang, B. J., Vuong, B. Q., Chu, G., Goff, S. P. (2002). Interaction between UV-damaged DNA Binding Activity Proteins and the c-Abl Tyrosine Kinase. J. Biol. Chem. 277: 34870-34878 [Abstract] [Full Text]
Rapic-Otrin, V., McLenigan, M. P., Bisi, D. C., Gonzalez, M., Levine, A. S. (2002). Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation. Nucleic Acids Res 30: 2588-2598 [Abstract] [Full Text]
Chen, X., Zhang, Y., Douglas, L., Zhou, P. (2001). UV-damaged DNA-binding Proteins Are Targets of CUL-4A-mediated Ubiquitination and Degradation. J. Biol. Chem. 276: 48175-48182 [Abstract] [Full Text]
Nag, A., Datta, A., Yoo, K., Bhattacharyya, D., Chakrabortty, A., Wang, X., Slagle, B. L., Costa, R. H., Raychaudhuri, P. (2001). DDB2 Induces Nuclear Accumulation of the Hepatitis B Virus X Protein Independently of Binding to DDB1. J. Virol. 75: 10383-10392 [Abstract] [Full Text]
Nag, A., Bondar, T., Shiv, S., Raychaudhuri, P. (2001). The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells. Mol. Cell. Biol. 21: 6738-6747 [Abstract] [Full Text]
Martinez, E., Palhan, V. B., Tjernberg, A., Lymar, E. S., Gamper, A. M., Kundu, T. K., Chait, B. T., Roeder, R. G. (2001). Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo. Mol. Cell. Biol. 21: 6782-6795 [Abstract] [Full Text]
Araújo, S. J., Nigg, E. A., Wood, R. D. (2001). Strong Functional Interactions of TFIIH with XPC and XPG in Human DNA Nucleotide Excision Repair, without a Preassembled Repairosome. Mol. Cell. Biol. 21: 2281-2291 [Abstract] [Full Text]
Hara, R., Mo, J., Sancar, A. (2000). DNA Damage in the Nucleosome Core Is Refractory to Repair by Human Excision Nuclease. Mol. Cell. Biol. 20: 9173-9181 [Abstract] [Full Text]
Neuwald, A. F., Poleksic, A. (2000). PSI-BLAST searches using hidden Markov models of structural repeats: prediction of an unusual sliding DNA clamp and of {beta}-propellers in UV-damaged DNA-binding protein. Nucleic Acids Res 28: 3570-3580 [Abstract] [Full Text]
Shiyanov, P., Nag, A., Raychaudhuri, P. (1999). Cullin 4A Associates with the UV-damaged DNA-binding Protein DDB. J. Biol. Chem. 274: 35309-35312 [Abstract] [Full Text]
Fujiwara, Y., Masutani, C., Mizukoshi, T., Kondo, J., Hanaoka, F., Iwai, S. (1999). Characterization of DNA Recognition by the Human UV-damaged DNA-binding Protein. J. Biol. Chem. 274: 20027-20033 [Abstract] [Full Text]
Shiyanov, P., Hayes, S. A., Donepudi, M., Nichols, A. F., Linn, S., Slagle, B. L., Raychaudhuri, P. (1999). The Naturally Occurring Mutants of DDB Are Impaired in Stimulating Nuclear Import of the p125 Subunit and E2F1-Activated Transcription. Mol. Cell. Biol. 19: 4935-4943 [Abstract] [Full Text]
Wakasugi, M., Sancar, A. (1999). Order of Assembly of Human DNA Repair Excision Nuclease. J. Biol. Chem. 274: 18759-18768 [Abstract] [Full Text]
de Laat, W. L., Jaspers, N. G.J., Hoeijmakers, J. H.J. (1999). Molecular mechanism of nucleotide excision repair. Genes Dev. 13: 768-785 [Full Text]
Nichols, A. F., Itoh, T., Graham, J. A., Liu, W., Yamaizumi, M., Linn, S. (2000). Human Damage-specific DNA-binding Protein p48. CHARACTERIZATION OF XPE MUTATIONS AND REGULATION FOLLOWING UV IRRADIATION. J. Biol. Chem. 275: 21422-21428 [Abstract] [Full Text]
Wakasugi, M., Shimizu, M., Morioka, H., Linn, S., Nikaido, O., Matsunaga, T. (2001). Damaged DNA-binding Protein DDB Stimulates the Excision of Cyclobutane Pyrimidine Dimers in Vitro in Concert with XPA and Replication Protein A. J. Biol. Chem. 276: 15434-15440 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals