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Mol Cell Biol, June 1998, p. 3191-3200, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Chimeric Subunit of Yeast Transcription Factor IIIC Forms a Subcomplex with tau 95

Nathalie Manaud, Rosalía Arrebola, Bénédicte Buffin-Meyer, Olivier Lefebvre, Hartmut Voss,dagger Michel Riva, Christine Conesa, and André Sentenac*

Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France

Received 20 January 1998/Returned for modification 25 February 1998/Accepted 5 March 1998

The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We report the isolation and characterization of TFC7, an essential gene encoding the 55-kDa polypeptide, tau 55, present in affinity-purified TFIIIC. tau 55 is a chimeric protein generated by an ancient chromosomal rearrangement. Its C-terminal half is essential for cell viability and sufficient to ensure TFIIIC function in DNA binding and transcription assays. The N-terminal half is nonessential and highly similar to a putative yeast protein encoded on another chromosome and to a cyanobacterial protein of unknown function. Partial deletions of the N-terminal domain impaired tau 55 function at a high temperature or in media containing glycerol or ethanol, suggesting a link between PolIII transcription and metabolic pathways. Interestingly, tau 55 was found, together with TFIIIC subunit tau 95, in a protein complex which was distinct from TFIIIC and which may play a role in the regulation of PolIII transcription, possibly in relation to cell metabolism.

* Corresponding author. Mailing address: Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France. Phone: 33 1 69 08 22 36. Fax: 33 1 69 08 47 12. E-mail: sentenac{at}dsvidf.cea.fr.

dagger Present address: LION Biosciences AG, 69120 Heidelberg, Germany.

Mol Cell Biol, June 1998, p. 3191-3200, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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