This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sewalt, R. G. A. B.
Right arrow Articles by Otte, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sewalt, R. G. A. B.
Right arrow Articles by Otte, A. P.

 Previous Article  |  Next Article 

Mol Cell Biol, June 1998, p. 3586-3595, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Interactions between the Mammalian Polycomb-Group Proteins Enx1/EZH2 and EED Suggests the Existence of Different Mammalian Polycomb-Group Protein Complexes

Richard G. A. B. Sewalt, Johan van der Vlag, Marco J. Gunster, Karien M. Hamer, Jan L. den Blaauwen, David P. E. Satijn, Thijs Hendrix, Roel van Driel, and Arie P. Otte*

E. C. Slater Instituut, BioCentrum Amsterdam, University of Amsterdam, 1018 TV Amsterdam, The Netherlands

Received 1 December 1997/Returned for modification 7 January 1998/Accepted 24 February 1998

In Drosophila melanogaster, the Polycomb-group (PcG) and trithorax-group (trxG) genes have been identified as repressors and activators, respectively, of gene expression. Both groups of genes are required for the stable transmission of gene expression patterns to progeny cells throughout development. Several lines of evidence suggest a functional interaction between the PcG and trxG proteins. For example, genetic evidence indicates that the enhancer of zeste [E(z)] gene can be considered both a PcG and a trxG gene. To better understand the molecular interactions in which the E(z) protein is involved, we performed a two-hybrid screen with Enx1/EZH2, a mammalian homolog of E(z), as the target. We report the identification of the human EED protein, which interacts with Enx1/EZH2. EED is the human homolog of eed, a murine PcG gene which has extensive homology with the Drosophila PcG gene extra sex combs (esc). Enx1/EZH2 and EED coimmunoprecipitate, indicating that they also interact in vivo. However, Enx1/EZH2 and EED do not coimmunoprecipitate with other human PcG proteins, such as HPC2 and BMI1. Furthermore, unlike HPC2 and BMI1, which colocalize in nuclear domains of U-2 OS osteosarcoma cells, Enx1/EZH2 and EED do not colocalize with HPC2 or BMI1. Our findings indicate that Enx1/EZH2 and EED are members of a class of PcG proteins that is distinct from previously described human PcG proteins.

* Corresponding author. Mailing address: E. C. Slater Instituut, BioCentrum Amsterdam, University of Amsterdam, Plantage Muiderracht 12, 1018 TV Amsterdam, The Netherlands. Phone: 31-20-5255115. Fax: 31-20-5255124. E-mail: arie.otte{at}chem.uva.nl.

Mol Cell Biol, June 1998, p. 3586-3595, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Karanikolas, B. D.W., Figueiredo, M. L., Wu, L. (2009). Polycomb Group Protein Enhancer of Zeste 2 Is an Oncogene That Promotes the Neoplastic Transformation of a Benign Prostatic Epithelial Cell Line. Mol Cancer Res 7: 1456-1465 [Abstract] [Full Text]  
  • Kanellopoulou, C., Muljo, S. A., Dimitrov, S. D., Chen, X., Colin, C., Plath, K., Livingston, D. M. (2009). X chromosome inactivation in the absence of Dicer. Proc. Natl. Acad. Sci. USA 106: 1122-1127 [Abstract] [Full Text]  
  • Dutton, A., Woodman, C. B., Chukwuma, M. B., Last, J. I. K., Wei, W., Vockerodt, M., Baumforth, K. R. N., Flavell, J. R., Rowe, M., Taylor, A. M. R., Young, L. S., Murray, P. G. (2007). Bmi-1 is induced by the Epstein-Barr virus oncogene LMP1 and regulates the expression of viral target genes in Hodgkin lymphoma cells. Blood 109: 2597-2603 [Abstract] [Full Text]  
  • Takada, Y., Isono, K.-i., Shinga, J., Turner, J. M. A., Kitamura, H., Ohara, O., Watanabe, G., Singh, P. B., Kamijo, T., Jenuwein, T., Burgoyne, P. S., Koseki, H. (2007). Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. Development 134: 579-590 [Abstract] [Full Text]  
  • Kim, S. Y., Paylor, S. W., Magnuson, T., Schumacher, A. (2006). Juxtaposed Polycomb complexes co-regulate vertebral identity. Development 133: 4957-4968 [Abstract] [Full Text]  
  • Kamminga, L. M., Bystrykh, L. V., de Boer, A., Houwer, S., Douma, J., Weersing, E., Dontje, B., de Haan, G. (2006). The Polycomb group gene Ezh2 prevents hematopoietic stem cell exhaustion. Blood 107: 2170-2179 [Abstract] [Full Text]  
  • Collett, K., Eide, G. E., Arnes, J., Stefansson, I. M., Eide, J., Braaten, A., Aas, T., Otte, A. P., Akslen, L. A. (2006). Expression of Enhancer of Zeste Homologue 2 Is Significantly Associated with Increased Tumor Cell Proliferation and Is a Marker of Aggressive Breast Cancer. Clin. Cancer Res. 12: 1168-1174 [Abstract] [Full Text]  
  • Hernandez-Munoz, I., Taghavi, P., Kuijl, C., Neefjes, J., van Lohuizen, M. (2005). Association of BMI1 with Polycomb Bodies Is Dynamic and Requires PRC2/EZH2 and the Maintenance DNA Methyltransferase DNMT1. Mol. Cell. Biol. 25: 11047-11058 [Abstract] [Full Text]  
  • Raman, J. D., Mongan, N. P., Tickoo, S. K., Boorjian, S. A., Scherr, D. S., Gudas, L. J. (2005). Increased Expression of the Polycomb Group Gene, EZH2, in Transitional Cell Carcinoma of the Bladder. Clin. Cancer Res. 11: 8570-8576 [Abstract] [Full Text]  
  • Kunath, T., Arnaud, D., Uy, G. D., Okamoto, I., Chureau, C., Yamanaka, Y., Heard, E., Gardner, R. L., Avner, P., Rossant, J. (2005). Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts. Development 132: 1649-1661 [Abstract] [Full Text]  
  • Attwooll, C., Oddi, S., Cartwright, P., Prosperini, E., Agger, K., Steensgaard, P., Wagener, C., Sardet, C., Moroni, M. C., Helin, K. (2005). A Novel Repressive E2F6 Complex Containing the Polycomb Group Protein, EPC1, That Interacts with EZH2 in a Proliferation-specific Manner. J. Biol. Chem. 280: 1199-1208 [Abstract] [Full Text]  
  • Plath, K., Talbot, D., Hamer, K. M., Otte, A. P., Yang, T. P., Jaenisch, R., Panning, B. (2004). Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome. JCB 167: 1025-1035 [Abstract] [Full Text]  
  • Dukers, D. F., van Galen, J. C., Giroth, C., Jansen, P., Sewalt, R. G.A.B., Otte, A. P., Kluin-Nelemans, H. C., Meijer, C. J.L.M., Raaphorst, F. M. (2004). Unique Polycomb Gene Expression Pattern in Hodgkin's Lymphoma and Hodgkin's Lymphoma-Derived Cell Lines. Am. J. Pathol. 164: 873-881 [Abstract] [Full Text]  
  • Raaphorst, F. M., Vermeer, M., Fieret, E., Blokzijl, T., Dukers, D., Sewalt, R. G.A.B., Otte, A. P., Willemze, R., Meijer, C. J.L.M. (2004). Site-Specific Expression of Polycomb-Group Genes Encoding the HPC-HPH/PRC1 Complex in Clinically Defined Primary Nodal and Cutaneous Large B-Cell Lymphomas. Am. J. Pathol. 164: 533-542 [Abstract] [Full Text]  
  • Violot, S., Hong, S. S., Rakotobe, D., Petit, C., Gay, B., Moreau, K., Billaud, G., Priet, S., Sire, J., Schwartz, O., Mouscadet, J.-F., Boulanger, P. (2003). The Human Polycomb Group EED Protein Interacts with the Integrase of Human Immunodeficiency Virus Type 1. J. Virol. 77: 12507-12522 [Abstract] [Full Text]  
  • Kleer, C. G., Cao, Q., Varambally, S., Shen, R., Ota, I., Tomlins, S. A., Ghosh, D., Sewalt, R. G. A. B., Otte, A. P., Hayes, D. F., Sabel, M. S., Livant, D., Weiss, S. J., Rubin, M. A., Chinnaiyan, A. M. (2003). EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells. Proc. Natl. Acad. Sci. USA 100: 11606-11611 [Abstract] [Full Text]  
  • Erhardt, S., Su, I-h., Schneider, R., Barton, S., Bannister, A. J., Perez-Burgos, L., Jenuwein, T., Kouzarides, T., Tarakhovsky, A., Surani, M. A. (2003). Consequences of the depletion of zygotic and embryonic enhancer of zeste 2 during preimplantation mouse development. Development 130: 4235-4248 [Abstract] [Full Text]  
  • Jin, Q., van Eynde, A., Beullens, M., Roy, N., Thiel, G., Stalmans, W., Bollen, M. (2003). The Protein Phosphatase-1 (PP1) Regulator, Nuclear Inhibitor of PP1 (NIPP1), Interacts with the Polycomb Group Protein, Embryonic Ectoderm Development (EED), and Functions as a Transcriptional Repressor. J. Biol. Chem. 278: 30677-30685 [Abstract] [Full Text]  
  • Xia, Z.-B., Anderson, M., Diaz, M. O., Zeleznik-Le, N. J. (2003). MLL repression domain interacts with histone deacetylases, the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein. Proc. Natl. Acad. Sci. USA 100: 8342-8347 [Abstract] [Full Text]  
  • Rhodes, D. R., Sanda, M. G., Otte, A. P., Chinnaiyan, A. M., Rubin, M. A. (2003). Multiplex Biomarker Approach for Determining Risk of Prostate-Specific Antigen-Defined Recurrence of Prostate Cancer. JNCI J Natl Cancer Inst 95: 661-668 [Abstract] [Full Text]  
  • Suzuki, M., Mizutani-Koseki, Y., Fujimura, Y.-i., Miyagishima, H., Kaneko, T., Takada, Y., Akasaka, T., Tanzawa, H., Takihara, Y., Nakano, M., Masumoto, H., Vidal, M., Isono, K.-i., Koseki, H. (2003). Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice. Development 129: 4171-4183 [Abstract] [Full Text]  
  • Voncken, J. W., Roelen, B. A. J., Roefs, M., de Vries, S., Verhoeven, E., Marino, S., Deschamps, J., van Lohuizen, M. (2003). Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition. Proc. Natl. Acad. Sci. USA 100: 2468-2473 [Abstract] [Full Text]  
  • Cmarko, D., Verschure, P. J., Otte, A. P., van Driel, R., Fakan, S. (2003). Polycomb group gene silencing proteins are concentrated in the perichromatin compartment of the mammalian nucleus. J. Cell Sci. 116: 335-343 [Abstract] [Full Text]  
  • Kuzmichev, A., Nishioka, K., Erdjument-Bromage, H., Tempst, P., Reinberg, D. (2002). Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein. Genes Dev. 16: 2893-2905 [Abstract] [Full Text]  
  • Dasilva, C., Hadji, H., Ozouf-Costaz, C., Nicaud, S., Jaillon, O., Weissenbach, J., Crollius, H. R. (2002). Remarkable compartmentalization of transposable elements and pseudogenes in the heterochromatin of the Tetraodon nigroviridis genome. Proc. Natl. Acad. Sci. USA 99: 13636-13641 [Abstract] [Full Text]  
  • Levine, S. S., Weiss, A., Erdjument-Bromage, H., Shao, Z., Tempst, P., Kingston, R. E. (2002). The Core of the Polycomb Repressive Complex Is Compositionally and Functionally Conserved in Flies and Humans. Mol. Cell. Biol. 22: 6070-6078 [Abstract] [Full Text]  
  • Sewalt, R. G. A. B., Lachner, M., Vargas, M., Hamer, K. M., den Blaauwen, J. L., Hendrix, T., Melcher, M., Schweizer, D., Jenuwein, T., Otte, A. P. (2002). Selective Interactions between Vertebrate Polycomb Homologs and the SUV39H1 Histone Lysine Methyltransferase Suggest that Histone H3-K9 Methylation Contributes to Chromosomal Targeting of Polycomb Group Proteins. Mol. Cell. Biol. 22: 5539-5553 [Abstract] [Full Text]  
  • Tuckfield, A., Clouston, D. R., Wilanowski, T. M., Zhao, L.-L., Cunningham, J. M., Jane, S. M. (2002). Binding of the RING Polycomb Proteins to Specific Target Genes in Complex with the grainyhead-Like Family of Developmental Transcription Factors. Mol. Cell. Biol. 22: 1936-1946 [Abstract] [Full Text]  
  • O'Connell, S., Wang, L., Robert, S., Jones, C. A., Saint, R., Jones, R. S. (2001). Polycomblike PHD Fingers Mediate Conserved Interaction with Enhancer of Zeste Protein. J. Biol. Chem. 276: 43065-43073 [Abstract] [Full Text]  
  • Poux, S., Melfi, R., Pirrotta, V. (2001). Establishment of Polycomb silencing requires a transient interaction between PC and ESC. Genes Dev. 15: 2509-2514 [Abstract] [Full Text]  
  • O'Carroll, D., Erhardt, S., Pagani, M., Barton, S. C., Surani, M. A., Jenuwein, T. (2001). The Polycomb-Group Gene Ezh2 Is Required for Early Mouse Development. Mol. Cell. Biol. 21: 4330-4336 [Abstract] [Full Text]  
  • van Kemenade, F. J., Raaphorst, F. M., Blokzijl, T., Fieret, E., Hamer, K. M., Satijn, D. P. E., Otte, A. P., Meijer, C. J. L. M. (2001). Coexpression of BMI-1 and EZH2 polycomb-group proteins is associated with cycling cells and degree of malignancy in B-cell non-Hodgkin lymphoma. Blood 97: 3896-3901 [Abstract] [Full Text]  
  • Raaphorst, F. M., Otte, A. P., van Kemenade, F. J., Blokzijl, T., Fieret, E., Hamer, K. M., Satijn, D. P. E., Meijer, C. J. L. M. (2001). Distinct BMI-1 and EZH2 Expression Patterns in Thymocytes and Mature T Cells Suggest a Role for Polycomb Genes in Human T Cell Differentiation. J. Immunol. 166: 5925-5934 [Abstract] [Full Text]  
  • Roseman, R. R., Morgan, K., Mallin, D. R., Roberson, R., Parnell, T. J., Bornemann, D. J., Simon, J. A., Geyer, P. K. (2001). Long-Range Repression by Multiple Polycomb Group (PcG) Proteins Targeted by Fusion to a Defined DNA-Binding Domain in Drosophila. Genetics 158: 291-307 [Abstract] [Full Text]  
  • Xu, L., Fong, Y., Strome, S. (2001). The Caenorhabditis elegans maternal-effect sterile proteins, MES-2, MES-3, and MES-6, are associated in a complex in embryos. Proc. Natl. Acad. Sci. USA 98: 5061-5066 [Abstract] [Full Text]  
  • Satijn, D. P. E., Hamer, K. M., den Blaauwen, J., Otte, A. P. (2001). The Polycomb Group Protein EED Interacts with YY1, and Both Proteins Induce Neural Tissue in Xenopus Embryos. Mol. Cell. Biol. 21: 1360-1369 [Abstract] [Full Text]  
  • Stauffer, D. R., Howard, T. L., Nyun, T., Hollenberg, S. M. (2001). CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression. J. Cell Sci. 114: 2383-2393 [Abstract] [Full Text]  
  • Akasaka, T, van Lohuizen, M, van der Lugt, N, Mizutani-Koseki, Y, Kanno, M, Taniguchi, M, Vidal, M, Alkema, M, Berns, A, Koseki, H (2001). Mice doubly deficient for the Polycomb Group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression. Development 128: 1587-1597 [Abstract]  
  • Tie, F, Furuyama, T, Prasad-Sinha, J, Jane, E, Harte, P. (2001). The Drosophila Polycomb Group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3. Development 128: 275-286 [Abstract]  
  • Yadegari, R., Kinoshita, T., Lotan, O., Cohen, G., Katz, A., Choi, Y., Katz, A., Nakashima, K., Harada, J. J., Goldberg, R. B., Fischer, R. L., Ohad, N. (2000). Mutations in the FIE and MEA Genes That Encode Interacting Polycomb Proteins Cause Parent-of-Origin Effects on Seed Development by Distinct Mechanisms. Plant Cell 12: 2367-2382 [Abstract] [Full Text]  
  • Raaphorst, F. M., van Kemenade, F. J., Blokzijl, T., Fieret, E., Hamer, K. M., Satijn, D. P. E., Otte, A. P., Meijer, C. J. L. M. (2000). Coexpression of BMI-1 and EZH2 Polycomb Group Genes in Reed-Sternberg Cells of Hodgkin's Disease. Am. J. Pathol. 157: 709-715 [Abstract] [Full Text]  
  • Shnyreva, M., Schullery, D. S., Suzuki, H., Higaki, Y., Bomsztyk, K. (2000). Interaction of Two Multifunctional Proteins. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K AND Y-BOX-BINDING PROTEIN. J. Biol. Chem. 275: 15498-15503 [Abstract] [Full Text]  
  • Ng, J., Hart, C. M., Morgan, K., Simon, J. A. (2000). A Drosophila ESC-E(Z) Protein Complex Is Distinct from Other Polycomb Group Complexes and Contains Covalently Modified ESC. Mol. Cell. Biol. 20: 3069-3078 [Abstract] [Full Text]  
  • Li, H., Leo, C., Zhu, J., Wu, X., O'Neil, J., Park, E.-J., Chen, J. D. (2000). Sequestration and Inhibition of Daxx-Mediated Transcriptional Repression by PML. Mol. Cell. Biol. 20: 1784-1796 [Abstract] [Full Text]  
  • Akhmanova, A, Verkerk, T, Langeveld, A, Grosveld, F, Galjart, N (2000). Characterisation of transcriptionally active and inactive chromatin domains in neurons. J. Cell Sci. 113: 4463-4474 [Abstract]  
  • Raaphorst, F. M., van Kemenade, F. J., Fieret, E., Hamer, K. M., Satijn, D. P. E., Otte, A. P., Meijer, C. J. L. M. (2000). Cutting Edge: Polycomb Gene Expression Patterns Reflect Distinct B Cell Differentiation Stages in Human Germinal Centers. J. Immunol. 164: 1-4 [Abstract] [Full Text]  
  • Lessard, J., Schumacher, A., Thorsteinsdottir, U., van Lohuizen, M., Magnuson, T., Sauvageau, G. (1999). Functional antagonism of the Polycomb-Group genes eed and Bmi1 in hemopoietic cell proliferation. Genes Dev. 13: 2691-2703 [Abstract] [Full Text]  
  • Strouboulis, J., Damjanovski, S., Vermaak, D., Meric, F., Wolffe, A. P. (1999). Transcriptional Repression by XPc1, a New Polycomb Homolog in Xenopus laevis Embryos, Is Independent of Histone Deacetylase. Mol. Cell. Biol. 19: 3958-3968 [Abstract] [Full Text]  
  • Kiyosue, T., Ohad, N., Yadegari, R., Hannon, M., Dinneny, J., Wells, D., Katz, A., Margossian, L., Harada, J. J., Goldberg, R. B., Fischer, R. L. (1999). Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis. Proc. Natl. Acad. Sci. USA 96: 4186-4191 [Abstract] [Full Text]  
  • Ohad, N., Yadegari, R., Margossian, L., Hannon, M., Michaeli, D., Harada, J. J., Goldberg, R. B., Fischer, R. L. (1999). Mutations in FIE, a WD Polycomb Group Gene, Allow Endosperm Development without Fertilization. Plant Cell 11: 407-416 [Abstract] [Full Text]  
  • Peytavi, R., Hong, S. S., Gay, B., d'Angeac, A. D., Selig, L., Benichou, S., Benarous, R., Boulanger, P. (1999). HEED, the Product of the Human Homolog of the Murine eed Gene, Binds to the Matrix Protein of HIV-1. J. Biol. Chem. 274: 1635-1645 [Abstract] [Full Text]  
  • Satijn, D. P. E., Otte, A. P. (1999). RING1 Interacts with Multiple Polycomb-Group Proteins and Displays Tumorigenic Activity. Mol. Cell. Biol. 19: 57-68 [Abstract] [Full Text]  
  • Sewalt, R. G. A. B., Gunster, M. J., van der Vlag, J., Satijn, D. P. E., Otte, A. P. (1999). C-Terminal Binding Protein Is a Transcriptional Repressor That Interacts with a Specific Class of Vertebrate Polycomb Proteins. Mol. Cell. Biol. 19: 777-787 [Abstract] [Full Text]  
  • Rietzler, M., Bittner, M., Kolanus, W., Schuster, A., Holzmann, B. (1998). The Human WD Repeat Protein WAIT-1 Specifically Interacts with the Cytoplasmic Tails of beta 7-Integrins. J. Biol. Chem. 273: 27459-27466 [Abstract] [Full Text]  
  • Tie, F, Furuyama, T, Harte, P. (1998). The Drosophila Polycomb Group proteins ESC and E(Z) bind directly to each other and co-localize at multiple chromosomal sites. Development 125: 3483-3496 [Abstract]  
  • Bardos, J. I., Saurin, A. J., Tissot, C., Duprez, E., Freemont, P. S. (2000). HPC3 Is a New Human Polycomb Orthologue That Interacts and Associates with RING1 and Bmi1 and Has Transcriptional Repression Properties. J. Biol. Chem. 275: 28785-28792 [Abstract] [Full Text]  
  • Springer, N. M., Danilevskaya, O. N., Hermon, P., Helentjaris, T. G., Phillips, R. L., Kaeppler, H. F., Kaeppler, S. M. (2002). Sequence Relationships, Conserved Domains, and Expression Patterns for Maize Homologs of the Polycomb Group Genes E(z), esc, and E(Pc). Plant Physiol. 128: 1332-1345 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»