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Mol Cell Biol, June 1998, p. 3586-3595, Vol. 18, No. 6
E. C. Slater Instituut, BioCentrum
Amsterdam, University of Amsterdam, 1018 TV Amsterdam, The
Netherlands
Received 1 December 1997/Returned for modification 7 January
1998/Accepted 24 February 1998
In Drosophila melanogaster, the
Polycomb-group (PcG) and
trithorax-group (trxG) genes have been
identified as repressors and activators, respectively, of gene
expression. Both groups of genes are required for the stable
transmission of gene expression patterns to progeny cells throughout
development. Several lines of evidence suggest a functional interaction
between the PcG and trxG proteins. For example, genetic evidence
indicates that the enhancer of zeste [E(z)]
gene can be considered both a PcG and a trxG
gene. To better understand the molecular interactions in which
the E(z) protein is involved, we performed a two-hybrid screen with
Enx1/EZH2, a mammalian homolog of E(z), as the target. We
report the identification of the human EED protein, which interacts
with Enx1/EZH2. EED is the human homolog of
eed, a murine PcG gene which has extensive homology with
the Drosophila PcG gene extra sex combs
(esc). Enx1/EZH2 and EED coimmunoprecipitate, indicating
that they also interact in vivo. However, Enx1/EZH2 and EED do not
coimmunoprecipitate with other human PcG proteins, such as HPC2 and
BMI1. Furthermore, unlike HPC2 and BMI1, which colocalize in nuclear
domains of U-2 OS osteosarcoma cells, Enx1/EZH2 and EED do not
colocalize with HPC2 or BMI1. Our findings indicate that
Enx1/EZH2 and EED are members of a class of PcG proteins that is
distinct from previously described human PcG proteins.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Characterization of Interactions between the
Mammalian Polycomb-Group Proteins Enx1/EZH2 and EED Suggests the
Existence of Different Mammalian Polycomb-Group Protein
Complexes
*
Corresponding author. Mailing address: E. C. Slater Instituut, BioCentrum Amsterdam, University of Amsterdam,
Plantage Muiderracht 12, 1018 TV Amsterdam, The Netherlands.
Phone: 31-20-5255115. Fax: 31-20-5255124. E-mail:
arie.otte{at}chem.uva.nl.
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