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Mol Cell Biol, June 1998, p. 3596-3603, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

p300/CREB Binding Protein-Related Protein p270 Is a Component of Mammalian SWI/SNF Complexes

Peter B. Dallas,1 Ian Wayne Cheney,1,dagger Da-Wei Liao,1 Valerie Bowrin,1 Whitney Byam,1 Stephen Pacchione,1 Ryuji Kobayashi,2 Peter Yaciuk,3 and Elizabeth Moran1,*

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 191401; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 117242; and St. Louis University School of Medicine, St. Louis, Missouri 631043

Received 14 August 1997/Returned for modification 2 October 1997/Accepted 11 March 1998

p300 and the closely related CREB binding protein (CBP) are transcriptional adaptors that are present in intracellular complexes with TATA binding protein (TBP) and bind to upstream activators including p53 and nuclear hormone receptors. They have intrinsic and associated histone acetyltransferase activity, suggesting that chromatin modification is an essential part of their role in regulating transcription. Detailed characterization of a panel of antibodies raised against p300/CBP has revealed the existence of a 270-kDa cellular protein, p270, distinct from p300 and CBP but sharing at least two independent epitopes with p300. The subset of p300/CBP-derived antibodies that cross-reacts with p270 consistently coprecipitates a series a cellular proteins with relative molecular masses ranging from 44 to 190 kDa. Purification and analysis of various proteins in this group reveals that they are components of the human SWI/SNF complex and that p270 is an integral member of this complex.

* Corresponding author. Mailing address: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 N. Broad St., Philadelphia, PA 19140. Phone: (215) 707-7313. Fax: (215) 707-6989. E-mail: betty{at}sgi1.fels.temple.edu.

dagger Present address: Canji, Inc., San Diego, CA 92121.

Mol Cell Biol, June 1998, p. 3596-3603, Vol. 18, No. 6
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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