This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Onishi, M.
Right arrow Articles by Kitamura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Onishi, M.
Right arrow Articles by Kitamura, T.

 Previous Article  |  Next Article 

Mol Cell Biol, July 1998, p. 3871-3879, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Characterization of a Constitutively Active STAT5 Mutant That Promotes Cell Proliferation

Mayumi Onishi,1 dagger Tetsuya Nosaka,2 Kazuhide Misawa,2 Alice L.-F. Mui,3 Daniel Gorman,3 Martin McMahon,1 Atsushi Miyajima,4 and Toshio Kitamura2 *

Departments of Cell Signaling1 and Molecular Biology,3 DNAX Research Institute of Molecular and Cell Biology, Palo Alto, California 94304, and Department of Hemopoietic Factors, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108,2 and Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113,4 Japan.

Received 1 December 1997/Returned for modification 27 January 1998/Accepted 31 March 1998

STAT (signal transducers and activators of transcription) proteins are transcription factors which are activated by phosphorylation on tyrosine residues upon stimulation by cytokines. Seven members of the STAT family are known, including the closely related STAT5A and STAT5B, which are activated by various cytokines. Except for prolactin-dependent beta -casein production in mammary gland cells, the biological consequences of STAT5 activation in various systems are not clear. We applied PCR-driven random mutagenesis and a retrovirus-mediated expression screening system to identify constitutively active forms of STAT5. By this strategy, we have identified a constitutively active STAT5 mutant which has two amino acid substitutions; one is located upstream of the putative DNA binding domain (H299R), and the other is located in the transactivation domain (S711F). The mutant STAT5 was constitutively phosphorylated on tyrosine residues, localized in the nucleus, and was transcriptionally active. Expression of the mutant STAT5 partially dispenses with interleukin 3 (IL-3) as a growth stimulant of IL-3-dependent cell lines. Further analyses of the mutant STAT5 have demonstrated that both of the mutations are required for nuclear localization, efficient transcriptional activation, and induction of IL-3-independent growth of an IL-3-dependent cell line, Ba/F3, and have indicated that a molecular basis for the constitutive activation is the stability of the phosphorylated form of the mutant STAT5.

* Corresponding author. Mailing address: Department of Hemopoietic Factors, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan. Phone: 81-3-5449-5758. Fax: 81-3-5449-5453. E-mail: kitamura{at}ims.u-tokyo.ac.jp.

dagger Present address: Third Department of Internal Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.

Mol Cell Biol, July 1998, p. 3871-3879, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tani-ichi, S., Satake, M., Ikuta, K. (2009). Activation of the mouse TCR{gamma} enhancers by STAT5. Int Immunol 21: 1079-1088 [Abstract] [Full Text]  
  • Funakoshi-Tago, M., Tago, K., Sumi, K., Abe, M., Aizu-Yokota, E., Oshio, T., Sonoda, Y., Kasahara, T. (2009). The Acute Lymphoblastic Leukemia-associated JAK2 L611S Mutant Induces Tumorigenesis in Nude Mice. J. Biol. Chem. 284: 12680-12690 [Abstract] [Full Text]  
  • Kawashima, T., Bao, Y. C., Minoshima, Y., Nomura, Y., Hatori, T., Hori, T., Fukagawa, T., Fukada, T., Takahashi, N., Nosaka, T., Inoue, M., Sato, T., Kukimoto-Niino, M., Shirouzu, M., Yokoyama, S., Kitamura, T. (2009). A Rac GTPase-Activating Protein, MgcRacGAP, Is a Nuclear Localizing Signal-Containing Nuclear Chaperone in the Activation of STAT Transcription Factors. Mol. Cell. Biol. 29: 1796-1813 [Abstract] [Full Text]  
  • Xu, J., Chen, S., Chen, H., Xiao, Q., Hsu, C. Y., Michael, D., Bao, J. (2009). STAT5 Mediates Antiapoptotic Effects of Methylprednisolone on Oligodendrocytes. J. Neurosci. 29: 2022-2026 [Abstract] [Full Text]  
  • Yang, X., Qiao, D., Meyer, K., Friedl, A. (2009). Signal Transducers and Activators of Transcription Mediate Fibroblast Growth Factor-Induced Vascular Endothelial Morphogenesis. Cancer Res. 69: 1668-1677 [Abstract] [Full Text]  
  • Xu, R., Nelson, C. M., Muschler, J. L., Veiseh, M., Vonderhaar, B. K., Bissell, M. J. (2009). Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function. JCB 184: 57-66 [Abstract] [Full Text]  
  • Dentelli, P., Trombetta, A., Togliatto, G., Zeoli, A., Rosso, A., Uberti, B., Orso, F., Taverna, D., Pegoraro, L., Brizzi, M. F. (2009). Formation of STAT5/PPAR{gamma} Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes. Arterioscler. Thromb. Vasc. Bio. 29: 114-120 [Abstract] [Full Text]  
  • Koppikar, P., Lui, V. W. Y., Man, D., Xi, S., Chai, R. L., Nelson, E., Tobey, A. B.J., Grandis, J. R. (2008). Constitutive Activation of Signal Transducer and Activator of Transcription 5 Contributes to Tumor Growth, Epithelial-Mesenchymal Transition, and Resistance to Epidermal Growth Factor Receptor Targeting. Clin. Cancer Res. 14: 7682-7690 [Abstract] [Full Text]  
  • Zhu, B.-M., McLaughlin, S. K., Na, R., Liu, J., Cui, Y., Martin, C., Kimura, A., Robinson, G. W., Andrews, N. C., Hennighausen, L. (2008). Hematopoietic-specific Stat5-null mice display microcytic hypochromic anemia associated with reduced transferrin receptor gene expression. Blood 112: 2071-2080 [Abstract] [Full Text]  
  • Maki, K., Ikuta, K. (2008). MEK1/2 Induces STAT5-Mediated Germline Transcription of the TCR{gamma} Locus in Response to IL-7R Signaling. J. Immunol. 181: 494-502 [Abstract] [Full Text]  
  • Ogawa, S., Satake, M., Ikuta, K. (2008). Physical and Functional Interactions between STAT5 and Runx Transcription Factors. J Biochem 143: 695-709 [Abstract] [Full Text]  
  • Grebien, F., Kerenyi, M. A., Kovacic, B., Kolbe, T., Becker, V., Dolznig, H., Pfeffer, K., Klingmuller, U., Muller, M., Beug, H., Mullner, E. W., Moriggl, R. (2008). Stat5 activation enables erythropoiesis in the absence of EpoR and Jak2. Blood 111: 4511-4522 [Abstract] [Full Text]  
  • Scheeren, F. A., Diehl, S. A., Smit, L. A., Beaumont, T., Naspetti, M., Bende, R. J., Blom, B., Karube, K., Ohshima, K., van Noesel, C. J. M., Spits, H. (2008). IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis. Blood 111: 4706-4715 [Abstract] [Full Text]  
  • Wernig, G., Gonneville, J. R., Crowley, B. J., Rodrigues, M. S., Reddy, M. M., Hudon, H. E., Walz, C., Reiter, A., Podar, K., Royer, Y., Constantinescu, S. N., Tomasson, M. H., Griffin, J. D., Gilliland, D. G., Sattler, M. (2008). The Jak2V617F oncogene associated with myeloproliferative diseases requires a functional FERM domain for transformation and for expression of the Myc and Pim proto-oncogenes. Blood 111: 3751-3759 [Abstract] [Full Text]  
  • Nie, L., Perry, S. S., Zhao, Y., Huang, J., Kincade, P. W., Farrar, M. A., Sun, X.-H. (2008). Regulation of Lymphocyte Development by Cell-Type-Specific Interpretation of Notch Signals. Mol. Cell. Biol. 28: 2078-2090 [Abstract] [Full Text]  
  • Passerini, L., Allan, S. E., Battaglia, M., Di Nunzio, S., Alstad, A. N., Levings, M. K., Roncarolo, M. G., Bacchetta, R. (2008). STAT5-signaling cytokines regulate the expression of FOXP3 in CD4+CD25+ regulatory T cells and CD4+CD25- effector T cells. Int Immunol 20: 421-431 [Abstract] [Full Text]  
  • Wofford, J. A., Wieman, H. L., Jacobs, S. R., Zhao, Y., Rathmell, J. C. (2008). IL-7 promotes Glut1 trafficking and glucose uptake via STAT5-mediated activation of Akt to support T-cell survival. Blood 111: 2101-2111 [Abstract] [Full Text]  
  • Mallette, F. A., Gaumont-Leclerc, M.-F., Huot, G., Ferbeyre, G. (2007). Myc Down-regulation as a Mechanism to Activate the Rb Pathway in STAT5A-induced Senescence. J. Biol. Chem. 282: 34938-34944 [Abstract] [Full Text]  
  • Lockyer, H. M., Tran, E., Nelson, B. H. (2007). STAT5 Is Essential for Akt/p70S6 Kinase Activity during IL-2-Induced Lymphocyte Proliferation. J. Immunol. 179: 5301-5308 [Abstract] [Full Text]  
  • Takeshita, S., Faccio, R., Chappel, J., Zheng, L., Feng, X., Weber, J. D., Teitelbaum, S. L., Ross, F. P. (2007). c-Fms Tyrosine 559 Is a Major Mediator of M-CSF-induced Proliferation of Primary Macrophages. J. Biol. Chem. 282: 18980-18990 [Abstract] [Full Text]  
  • Zeng, R., Spolski, R., Casas, E., Zhu, W., Levy, D. E., Leonard, W. J. (2007). The molecular basis of IL-21-mediated proliferation. Blood 109: 4135-4142 [Abstract] [Full Text]  
  • Bao, L., Tessier, C., Prigent-Tessier, A., Li, F., Buzzio, O. L., Callegari, E. A., Horseman, N. D., Gibori, G. (2007). Decidual Prolactin Silences the Expression of Genes Detrimental to Pregnancy. Endocrinology 148: 2326-2334 [Abstract] [Full Text]  
  • Cain, J. A., Xiang, Z., O'Neal, J., Kreisel, F., Colson, A., Luo, H., Hennighausen, L., Tomasson, M. H. (2007). Myeloproliferative disease induced by TEL-PDGFRB displays dynamic range sensitivity to Stat5 gene dosage. Blood 109: 3906-3914 [Abstract] [Full Text]  
  • Buitenhuis, M., Verhagen, L. P., Cools, J., Coffer, P. J. (2007). Molecular Mechanisms Underlying FIP1L1-PDGFRA-Mediated Myeloproliferation. Cancer Res. 67: 3759-3766 [Abstract] [Full Text]  
  • Yang, X. O., Panopoulos, A. D., Nurieva, R., Chang, S. H., Wang, D., Watowich, S. S., Dong, C. (2007). STAT3 Regulates Cytokine-mediated Generation of Inflammatory Helper T Cells. J. Biol. Chem. 282: 9358-9363 [Abstract] [Full Text]  
  • Harir, N., Pecquet, C., Kerenyi, M., Sonneck, K., Kovacic, B., Nyga, R., Brevet, M., Dhennin, I., Gouilleux-Gruart, V., Beug, H., Valent, P., Lassoued, K., Moriggl, R., Gouilleux, F. (2007). Constitutive activation of Stat5 promotes its cytoplasmic localization and association with PI3-kinase in myeloid leukemias. Blood 109: 1678-1686 [Abstract] [Full Text]  
  • Feng, Y., Zhao, H., Luderer, H. F., Epple, H., Faccio, R., Ross, F. P., Teitelbaum, S. L., Longmore, G. D. (2007). The LIM Protein, LIMD1, Regulates AP-1 Activation through an Interaction with TRAF6 to Influence Osteoclast Development. J. Biol. Chem. 282: 39-48 [Abstract] [Full Text]  
  • Ciccaglione, A. R., Stellacci, E., Marcantonio, C., Muto, V., Equestre, M., Marsili, G., Rapicetta, M., Battistini, A. (2007). Repression of Interferon Regulatory Factor 1 by Hepatitis C Virus Core Protein Results in Inhibition of Antiviral and Immunomodulatory Genes. J. Virol. 81: 202-214 [Abstract] [Full Text]  
  • Mallette, F. A., Gaumont-Leclerc, M.-F., Ferbeyre, G. (2007). The DNA damage signaling pathway is a critical mediator of oncogene-induced senescence. Genes Dev. 21: 43-48 [Abstract] [Full Text]  
  • Kawashima, T., Bao, Y. C., Nomura, Y., Moon, Y., Tonozuka, Y., Minoshima, Y., Hatori, T., Tsuchiya, A., Kiyono, M., Nosaka, T., Nakajima, H., Williams, D. A., Kitamura, T. (2006). Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. JCB 175: 937-946 [Abstract] [Full Text]  
  • Zhou, P., Kitaura, H., Teitelbaum, S. L., Krystal, G., Ross, F. P., Takeshita, S. (2006). SHIP1 Negatively Regulates Proliferation of Osteoclast Precursors via Akt-Dependent Alterations in D-Type Cyclins and p27. J. Immunol. 177: 8777-8784 [Abstract] [Full Text]  
  • Miyaoka, Y., Tanaka, M., Naiki, T., Miyajima, A. (2006). Oncostatin M Inhibits Adipogenesis through the RAS/ERK and STAT5 Signaling Pathways. J. Biol. Chem. 281: 37913-37920 [Abstract] [Full Text]  
  • Yagi, J., Arimura, Y., Takatori, H., Nakajima, H., Iwamoto, I., Uchiyama, T. (2006). Genetic background influences Th cell differentiation by controlling the capacity for IL-2-induced IL-4 production by naive CD4+ T cells. Int Immunol 18: 1681-1690 [Abstract] [Full Text]  
  • Jackerott, M., Moldrup, A., Thams, P., Galsgaard, E. D., Knudsen, J., Lee, Y. C., Nielsen, J. H. (2006). STAT5 Activity in Pancreatic {beta}-Cells Influences the Severity of Diabetes in Animal Models of Type 1 and 2 Diabetes.. Diabetes 55: 2705-2712 [Abstract] [Full Text]  
  • Nelson, E. A., Walker, S. R., Li, W., Liu, X. S., Frank, D. A. (2006). Identification of Human STAT5-dependent Gene Regulatory Elements Based on Interspecies Homology. J. Biol. Chem. 281: 26216-26224 [Abstract] [Full Text]  
  • Ikeda, H., Okazawa, H., Ohnishi, H., Murata, Y., Oldenborg, P.-A., Matozaki, T. (2006). Mutational analysis of the mechanism of negative regulation by SRC homology 2 domain-containing protein tyrosine phosphatase substrate-1 of phagocytosis in macrophages.. J. Immunol. 177: 3123-3132 [Abstract] [Full Text]  
  • Zorn, E., Nelson, E. A., Mohseni, M., Porcheray, F., Kim, H., Litsa, D., Bellucci, R., Raderschall, E., Canning, C., Soiffer, R. J., Frank, D. A., Ritz, J. (2006). IL-2 regulates FOXP3 expression in human CD4+CD25+ regulatory T cells through a STAT-dependent mechanism and induces the expansion of these cells in vivo. Blood 108: 1571-1579 [Abstract] [Full Text]  
  • Garcon, L., Rivat, C., James, C., Lacout, C., Camara-Clayette, V., Ugo, V., Lecluse, Y., Bennaceur-Griscelli, A., Vainchenker, W. (2006). Constitutive activation of STAT5 and Bcl-xL overexpression can induce endogenous erythroid colony formation in human primary cells. Blood 108: 1551-1554 [Abstract] [Full Text]  
  • Chen, Y., Dai, X., Haas, A. L., Wen, R., Wang, D. (2006). Proteasome-dependent down-regulation of activated Stat5A in the nucleus. Blood 108: 566-574 [Abstract] [Full Text]  
  • Walz, C., Crowley, B. J., Hudon, H. E., Gramlich, J. L., Neuberg, D. S., Podar, K., Griffin, J. D., Sattler, M. (2006). Activated Jak2 with the V617F Point Mutation Promotes G1/S Phase Transition. J. Biol. Chem. 281: 18177-18183 [Abstract] [Full Text]  
  • Wierenga, A. T. J., Schepers, H., Moore, M. A. S., Vellenga, E., Schuringa, J. J. (2006). STAT5-induced self-renewal and impaired myelopoiesis of human hematopoietic stem/progenitor cells involves down-modulation of C/EBP{alpha}. Blood 107: 4326-4333 [Abstract] [Full Text]  
  • Ito, T., Arimitsu, N., Takeuchi, M., Kawamura, N., Nagata, M., Saso, K., Akimitsu, N., Hamamoto, H., Natori, S., Miyajima, A., Sekimizu, K. (2006). Transcription Elongation Factor S-II Is Required for Definitive Hematopoiesis. Mol. Cell. Biol. 26: 3194-3203 [Abstract] [Full Text]  
  • Jensen, J., Galsgaard, E. D, Karlsen, A. E, Lee, Y. C, Nielsen, J. H (2005). STAT5 activation by human GH protects insulin-producing cells against interleukin-1{beta}, interferon-{gamma} and tumour necrosis factor-{alpha}-induced apoptosis independent of nitric oxide production. J Endocrinol 187: 25-36 [Abstract] [Full Text]  
  • Zhuang, D., Qiu, Y., Haque, S. J., Dong, F. (2005). Tyrosine 729 of the G-CSF receptor controls the duration of receptor signaling: involvement of SOCS3 and SOCS1. J. Leukoc. Biol. 78: 1008-1015 [Abstract] [Full Text]  
  • Kato, Y., Iwama, A., Tadokoro, Y., Shimoda, K., Minoguchi, M., Akira, S., Tanaka, M., Miyajima, A., Kitamura, T., Nakauchi, H. (2005). Selective activation of STAT5 unveils its role in stem cell self-renewal in normal and leukemic hematopoiesis. JEM 202: 169-179 [Abstract] [Full Text]  
  • Ezoe, S., Matsumura, I., Gale, K., Satoh, Y., Ishikawa, J., Mizuki, M., Takahashi, S., Minegishi, N., Nakajima, K., Yamamoto, M., Enver, T., Kanakura, Y. (2005). GATA Transcription Factors Inhibit Cytokine-dependent Growth and Survival of a Hematopoietic Cell Line through the Inhibition of STAT3 Activity. J. Biol. Chem. 280: 13163-13170 [Abstract] [Full Text]  
  • Defilippi, P., Rosso, A., Dentelli, P., Calvi, C., Garbarino, G., Tarone, G., Pegoraro, L., Brizzi, M. F. (2005). {beta}1 integrin and IL-3R coordinately regulate STAT5 activation and anchorage-dependent proliferation. JCB 168: 1099-1108 [Abstract] [Full Text]  
  • Stanton, M. L., Brodeur, P. H. (2005). Cutting Edge: Stat5 Mediates the IL-7-Induced Accessibility of a Representative D-Distal VH Gene. J. Immunol. 174: 3164-3168 [Abstract] [Full Text]  
  • Sekine, Y., Yamamoto, T., Yumioka, T., Sugiyama, K., Tsuji, S., Oritani, K., Shimoda, K., Minoguchi, M., Yoshimura, A., Matsuda, T. (2005). Physical and Functional Interactions between STAP-2/BKS and STAT5. J. Biol. Chem. 280: 8188-8196 [Abstract] [Full Text]  
  • Zhao, H., Ross, F. P., Teitelbaum, S. L. (2005). Unoccupied {alpha}v{beta}3 Integrin Regulates Osteoclast Apoptosis by Transmitting a Positive Death Signal. Mol. Endocrinol. 19: 771-780 [Abstract] [Full Text]  
  • Kohno, M., Yamasaki, S., Tybulewicz, V. L. J., Saito, T. (2005). Rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. Blood 105: 2059-2065 [Abstract] [Full Text]  
  • Liu, W., Xu, D., Yang, H., Xu, H., Shi, Z., Cao, X., Takeshita, S., Liu, J., Teale, M., Feng, X. (2004). Functional Identification of Three Receptor Activator of NF-{kappa}B Cytoplasmic Motifs Mediating Osteoclast Differentiation and Function. J. Biol. Chem. 279: 54759-54769 [Abstract] [Full Text]  
  • Clevenger, C. V. (2004). Roles and Regulation of Stat Family Transcription Factors in Human Breast Cancer. Am. J. Pathol. 165: 1449-1460 [Abstract] [Full Text]  
  • Schuringa, J. J., Chung, K. Y., Morrone, G., Moore, M. A.S. (2004). Constitutive Activation of STAT5A Promotes Human Hematopoietic Stem Cell Self-Renewal and Erythroid Differentiation. JEM 200: 623-635 [Abstract] [Full Text]  
  • Jiang, Q., Li, W. Q., Hofmeister, R. R., Young, H. A., Hodge, D. R., Keller, J. R., Khaled, A. R., Durum, S. K. (2004). Distinct Regions of the Interleukin-7 Receptor Regulate Different Bcl2 Family Members. Mol. Cell. Biol. 24: 6501-6513 [Abstract] [Full Text]  
  • Peltola, K. J., Paukku, K., Aho, T. L. T., Ruuska, M., Silvennoinen, O., Koskinen, P. J. (2004). Pim-1 kinase inhibits STAT5-dependent transcription via its interactions with SOCS1 and SOCS3. Blood 103: 3744-3750 [Abstract] [Full Text]  
  • Goetz, C. A., Harmon, I. R., O'Neil, J. J., Burchill, M. A., Farrar, M. A. (2004). STAT5 Activation Underlies IL7 Receptor-Dependent B Cell Development. J. Immunol. 172: 4770-4778 [Abstract] [Full Text]  
  • Ohki, R., Ishikawa, F. (2004). Telomere-bound TRF1 and TRF2 stall the replication fork at telomeric repeats. Nucleic Acids Res 32: 1627-1637 [Abstract] [Full Text]  
  • Shibayama, H., Takai, E., Matsumura, I., Kouno, M., Morii, E., Kitamura, Y., Takeda, J., Kanakura, Y. (2004). Identification of a Cytokine-induced Antiapoptotic Molecule Anamorsin Essential for Definitive Hematopoiesis. JEM 199: 581-592 [Abstract] [Full Text]  
  • Lin, C.-C., Chou, C.-M., Hsu, Y.-L., Lien, J.-C., Wang, Y.-M., Chen, S.-T., Tsai, S.-C., Hsiao, P.-W., Huang, C.-J. (2004). Characterization of Two Mosquito STATs, AaSTAT and CtSTAT: DIFFERENTIAL REGULATION OF TYROSINE PHOSPHORYLATION AND DNA BINDING ACTIVITY BY LIPOPOLYSACCHARIDE TREATMENT AND BY JAPANESE ENCEPHALITIS VIRUS INFECTION. J. Biol. Chem. 279: 3308-3317 [Abstract] [Full Text]  
  • Sternberg, D. W., Gilliland, D. G. (2004). The Role of Signal Transducer and Activator of Transcription Factors in Leukemogenesis. JCO 22: 361-371 [Abstract] [Full Text]  
  • Nagafuku, M., Kabayama, K., Oka, D., Kato, A., Tani-ichi, S., Shimada, Y., Ohno-Iwashita, Y., Yamasaki, S., Saito, T., Iwabuchi, K., Hamaoka, T., Inokuchi, J.-i., Kosugi, A. (2003). Reduction of Glycosphingolipid Levels in Lipid Rafts Affects the Expression State and Function of Glycosylphosphatidylinositol-anchored Proteins but Does Not Impair Signal Transduction via the T Cell Receptor. J. Biol. Chem. 278: 51920-51927 [Abstract] [Full Text]  
  • Magne, S., Caron, S., Charon, M., Rouyez, M.-C., Dusanter-Fourt, I. (2003). STAT5 and Oct-1 Form a Stable Complex That Modulates Cyclin D1 Expression. Mol. Cell. Biol. 23: 8934-8945 [Abstract] [Full Text]  
  • Russell, T. D., Yan, Q., Fan, G., Khalifah, A. P., Bishop, D. K., Brody, S. L., Walter, M. J. (2003). IL-12 p40 Homodimer-Dependent Macrophage Chemotaxis and Respiratory Viral Inflammation Are Mediated through IL-12 Receptor {beta}1. J. Immunol. 171: 6866-6874 [Abstract] [Full Text]  
  • Rascle, A., Lees, E. (2003). Chromatin acetylation and remodeling at the Cis promoter during STAT5-induced transcription. Nucleic Acids Res 31: 6882-6890 [Abstract] [Full Text]  
  • Shang, C. A., Waters, M. J. (2003). Constitutively Active Signal Transducer and Activator of Transcription 5 Can Replace the Requirement for Growth Hormone in Adipogenesis of 3T3-F442A Preadipocytes. Mol. Endocrinol. 17: 2494-2508 [Abstract] [Full Text]  
  • Burchill, M. A., Goetz, C. A., Prlic, M., O'Neil, J. J., Harmon, I. R., Bensinger, S. J., Turka, L. A., Brennan, P., Jameson, S. C., Farrar, M. A. (2003). Distinct Effects of STAT5 Activation on CD4+ and CD8+ T Cell Homeostasis: Development of CD4+CD25+ Regulatory T Cells versus CD8+ Memory T Cells. J. Immunol. 171: 5853-5864 [Abstract] [Full Text]  
  • Kataoka, Y., Matsumura, I., Ezoe, S., Nakata, S., Takigawa, E., Sato, Y., Kawasaki, A., Yokota, T., Nakajima, K., Felsani, A., Kanakura, Y. (2003). Reciprocal Inhibition between MyoD and STAT3 in the Regulation of Growth and Differentiation of Myoblasts. J. Biol. Chem. 278: 44178-44187 [Abstract] [Full Text]  
  • Xi, S., Zhang, Q., Gooding, W. E., Smithgall, T. E., Grandis, J. R. (2003). Constitutive Activation of Stat5b Contributes to Carcinogenesis in Vivo. Cancer Res. 63: 6763-6771 [Abstract] [Full Text]  
  • Antov, A., Yang, L., Vig, M., Baltimore, D., Van Parijs, L. (2003). Essential Role for STAT5 Signaling in CD25+CD4+ Regulatory T Cell Homeostasis and the Maintenance of Self-Tolerance. J. Immunol. 171: 3435-3441 [Abstract] [Full Text]  
  • Ito, T., Okazawa, H., Maruyama, K., Tomizawa, K., Motegi, S.-i., Ohnishi, H., Kuwano, H., Kosugi, A., Matozaki, T. (2003). Interaction of SAP-1, a Transmembrane-type Protein-tyrosine Phosphatase, with the Tyrosine Kinase Lck: ROLES IN REGULATION OF T CELL FUNCTION. J. Biol. Chem. 278: 34854-34863 [Abstract] [Full Text]  
  • Novack, D. V., Yin, L., Hagen-Stapleton, A., Schreiber, R. D., Goeddel, D. V., Ross, F. P., Teitelbaum, S. L. (2003). The I{kappa}B Function of NF-{kappa}B2 p100 Controls Stimulated Osteoclastogenesis. JEM 198: 771-781 [Abstract] [Full Text]  
  • Liu, Q.-L., Kishi, H., Ohtsuka, K., Muraguchi, A. (2003). Heat shock protein 70 binds caspase-activated DNase and enhances its activity in TCR-stimulated T cells. Blood 102: 1788-1796 [Abstract] [Full Text]  
  • Murata, K., Kumagai, H., Kawashima, T., Tamitsu, K., Irie, M., Nakajima, H., Suzu, S., Shibuya, M., Kamihira, S., Nosaka, T., Asano, S., Kitamura, T. (2003). Selective Cytotoxic Mechanism of GTP-14564, a Novel Tyrosine Kinase Inhibitor in Leukemia Cells Expressing a Constitutively Active Fms-like Tyrosine Kinase 3 (FLT3). J. Biol. Chem. 278: 32892-32898 [Abstract] [Full Text]  
  • McBride, K. M., Reich, N. C. (2003). The Ins and Outs of STAT1 Nuclear Transport. Sci Signal 2003: re13-re13 [Abstract] [Full Text]  
  • Shipley, J. M., Waxman, D. J. (2003). Down-Regulation of STAT5b Transcriptional Activity by Ligand-Activated Peroxisome Proliferator-Activated Receptor (PPAR) {alpha} and PPAR{gamma}. Mol. Pharmacol. 64: 355-364 [Abstract] [Full Text]  
  • Gomez, D., Reich, N. C. (2003). Stimulation of Primary Human Endothelial Cell Proliferation by IFN. J. Immunol. 170: 5373-5381 [Abstract] [Full Text]  
  • Friedrichsen, B. N., Richter, H. E., Hansen, J. A., Rhodes, C. J., Nielsen, J. H., Billestrup, N., Moldrup, A. (2003). Signal Transducer and Activator of Transcription 5 Activation Is Sufficient to Drive Transcriptional Induction of Cyclin D2 Gene and Proliferation of Rat Pancreatic {beta}-Cells. Mol. Endocrinol. 17: 945-958 [Abstract] [Full Text]  
  • Kunisato, A., Chiba, S., Nakagami-Yamaguchi, E., Kumano, K., Saito, T., Masuda, S., Yamaguchi, T., Osawa, M., Kageyama, R., Nakauchi, H., Nishikawa, M., Hirai, H. (2003). HES-1 preserves purified hematopoietic stem cells ex vivo and accumulates side population cells in vivo. Blood 101: 1777-1783 [Abstract] [Full Text]  
  • Tanimura, N., Nagafuku, M., Minaki, Y., Umeda, Y., Hayashi, F., Sakakura, J., Kato, A., Liddicoat, D. R., Ogata, M., Hamaoka, T., Kosugi, A. (2003). Dynamic changes in the mobility of LAT in aggregated lipid rafts upon T cell activation. JCB 160: 125-135 [Abstract] [Full Text]  
  • Koh, E. Y., Chen, T., Daley, G. Q. (2002). Novel retroviral vectors to facilitate expression screens in mammalian cells. Nucleic Acids Res 30: e142-e142 [Abstract] [Full Text]  
  • Si, J., Collins, S. J. (2002). IL-3-induced enhancement of retinoic acid receptor activity is mediated through Stat5, which physically associates with retinoic acid receptors in an IL-3-dependent manner. Blood 100: 4401-4409 [Abstract] [Full Text]  
  • Feng, X., Takeshita, S., Namba, N., Wei, S., Teitelbaum, S. L., Ross, F. P. (2002). Tyrosines 559 and 807 in the Cytoplasmic Tail of the Macrophage Colony-Stimulating Factor Receptor Play Distinct Roles in Osteoclast Differentiation and Function. Endocrinology 143: 4868-4874 [Abstract] [Full Text]  
  • Jin, Z.-X., Kishi, H., Wei, X.-C., Matsuda, T., Saito, S., Muraguchi, A. (2002). Lymphoid Enhancer-Binding Factor-1 Binds and Activates the Recombination-Activating Gene-2 Promoter Together with c-Myb and Pax-5 in Immature B Cells. J. Immunol. 169: 3783-3792 [Abstract] [Full Text]  
  • Tsuruyama, T., Nakamura, T., Jin, G., Ozeki, M., Yamada, Y., Hiai, H. (2002). Constitutive activation of Stat5a by retrovirus integration in early pre-B lymphomas of SL/Kh strain mice. Proc. Natl. Acad. Sci. USA 99: 8253-8258 [Abstract] [Full Text]  
  • Mak, I. Y. H., Brosens, J. J., Christian, M., Hills, F. A., Chamley, L., Regan, L., White, J. O. (2002). Regulated Expression of Signal Transducer and Activator of Transcription, Stat5, and its Enhancement of PRL Expression in Human Endometrial Stromal Cells in Vitro. J. Clin. Endocrinol. Metab. 87: 2581-2588 [Abstract] [Full Text]  
  • Buettner, R., Mora, L. B., Jove, R. (2002). Activated STAT Signaling in Human Tumors Provides Novel Molecular Targets for Therapeutic Intervention. Clin. Cancer Res. 8: 945-954 [Abstract] [Full Text]  
  • Nguyen, K. T., Zong, C. S., Uttamsingh, S., Sachdev, P., Bhanot, M., Le, M.-T., Chan, J. L.-K., Wang, L.-H. (2002). The Role of Phosphatidylinositol 3-Kinase, Rho Family GTPases, and STAT3 in Ros-induced Cell Transformation. J. Biol. Chem. 277: 11107-11115 [Abstract] [Full Text]  
  • Kunstle, G., Laine, J., Pierron, G., Kagami, S.-i., Nakajima, H., Hoh, F., Roumestand, C., Stern, M.-H., Noguchi, M. (2002). Identification of Akt Association and Oligomerization Domains of the Akt Kinase Coactivator TCL1. Mol. Cell. Biol. 22: 1513-1525 [Abstract] [Full Text]  
  • Nakamura, T., Ouchida, R., Kodama, T., Kawashima, T., Makino, Y., Yoshikawa, N., Watanabe, S., Morimoto, C., Kitamura, T., Tanaka, H. (2002). Cytokine Receptor Common beta Subunit-mediated STAT5 Activation Confers NF-kappa B Activation in Murine proB Cell Line Ba/F3 Cells. J. Biol. Chem. 277: 6254-6265 [Abstract] [Full Text]  
  • Frasor, J., Park, K., Byers, M., Telleria, C., Kitamura, T., Yu-Lee, L.-y., Djiane, J., Park-Sarge, O.-K., Gibori, G. (2001). Differential Roles for Signal Transducers and Activators of Transcription 5a and 5b in PRL Stimulation of ER{alpha} and ER{beta} Transcription. Mol. Endocrinol. 15: 2172-2181 [Abstract] [Full Text]  
  • Kumano, K., Chiba, S., Shimizu, K., Yamagata, T., Hosoya, N., Saito, T., Takahashi, T., Hamada, Y., Hirai, H. (2001). Notch1 inhibits differentiation of hematopoietic cells by sustaining GATA-2 expression. Blood 98: 3283-3289 [Abstract] [Full Text]  
  • Anderson, S. P., Dunn, C. S., Cattley, R. C., Corton, J.C. (2001). Hepatocellular proliferation in response to a peroxisome proliferator does not require TNF{alpha} signaling. Carcinogenesis 22: 1843-1851 [Abstract] [Full Text]  
  • Federico, M., Percario, Z., Olivetta, E., Fiorucci, G., Muratori, C., Micheli, A., Romeo, G., Affabris, E. (2001). HIV-1 Nef activates STAT1 in human monocytes/macrophages through the release of soluble factors. Blood 98: 2752-2761 [Abstract] [Full Text]  
  • Kawashima, T., Murata, K., Akira, S., Tonozuka, Y., Minoshima, Y., Feng, S., Kumagai, H., Tsuruga, H., Ikeda, Y., Asano, S., Nosaka, T., Kitamura, T. (2001). STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-{kappa}B p65. J. Immunol. 167: 3652-3660 [Abstract] [Full Text]  
  • Miki, H., Yamauchi, T., Suzuki, R., Komeda, K., Tsuchida, A., Kubota, N., Terauchi, Y., Kamon, J., Kaburagi, Y., Matsui, J., Akanuma, Y., Nagai, R., Kimura, S., Tobe, K., Kadowaki, T. (2001). Essential Role of Insulin Receptor Substrate 1 (IRS-1) and IRS-2 in Adipocyte Differentiation. Mol. Cell. Biol. 21: 2521-2532 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»