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Mol Cell Biol, July 1998, p. 3880-3888, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Schistosome Satellite DNA Encodes Active
Hammerhead Ribozymes
Gerardo
Ferbeyre,1
James M.
Smith,2 and
Robert
Cedergren1 *
Département de Biochimie,
Université de Montréal, Montréal, Québec,
Canada H3C 3J7,1 and
Institute of
Parasitology, Macdonald Campus of McGill University,
Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V92
Received 11 February 1998/Returned for modification 2 April
1998/Accepted 20 April 1998
Using a computer program designed to search for RNA structural
motifs in sequence databases, we have found a hammerhead ribozyme domain encoded in the Sm
repetitive DNA of Schistosoma
mansoni. Transcripts of these repeats are expressed as long
multimeric precursor RNAs that cleave in vitro and in vivo into
unit-length fragments. This RNA domain is able to engage in both
cis and trans cleavage typical of the
hammerhead ribozyme. Further computer analysis of S. mansoni DNA identified a potential trans cleavage site in the gene coding for a synaptobrevin-like protein, and RNA
transcribed from this gene was efficiently cleaved by the Sm
ribozyme in vitro. Similar families of repeats containing the
hammerhead domain were found in the closely related Schistosoma haematobium and Schistosomatium douthitti species but
were not present in Schistosoma japonicum or
Heterobilharzia americana, suggesting that the hammerhead
domain was not acquired from a common schistosome ancestor.
*
Corresponding author. Mailing address:
Département de Biochimie, Université de Montréal,
C. P. 6128, succursale Centre-Ville, Montréal, Québec,
Canada H3C 3J7. Phone: (514) 343-6320. Fax: (514) 343-2210. E-mail:
ceder{at}poste.umontreal.ca.

Present address: Cold Spring Harbor Laboratory, Cold Spring Harbor,
NY 11724.
Mol Cell Biol, July 1998, p. 3880-3888, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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