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Mol Cell Biol, July 1998, p. 3889-3899, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differential Requirements for Alternative Splicing and Nuclear Export Functions of Equine Infectious Anemia Virus Rev Protein

Matthew E. Harris,1 Richard R. Gontarek,2 dagger David Derse,2 and Thomas J. Hope1 *

Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037,1 and Laboratory of Leukocyte Biology, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 217022

Received 20 February 1998/Returned for modification 23 March 1998/Accepted 17 April 1998

The Rev protein of equine infectious anemia virus (ERev) exports unspliced and partially spliced viral RNAs from the nucleus. Like several cellular proteins, ERev regulates its own mRNA by mediating an alternative splicing event. To determine the requirements for these functions, we have identified ERev mutants that affect RNA export or both export and alternative splicing. Mutants were further characterized for subcellular localization, nuclear-cytoplasmic shuttling, and multimerization. None of the nuclear export signal (NES) mutants are defective for alternative splicing. Furthermore, the NES of ERev is similar in composition but distinct in spacing from other leucine-rich NESs. Basic residues at the C terminus of ERev are involved in nuclear localization, and disruption of the C-terminal residues affects both functions of ERev. ERev forms multimers, and no mutation disrupts this activity. In two mutants with substitutions of charged residues in the middle of ERev, RNA export is affected. One of these mutants is also defective for ERev-mediated alternative splicing but is identical to wild-type ERev in its localization, shuttling, and multimerization. Together, these results demonstrate that the two functions of ERev both require nuclear import and at least one other common activity, but RNA export can be separated from alternative splicing based on its requirement for a functional NES.

* Corresponding author. Mailing address: Infectious Disease Laboratory, The Salk Institute for Biological Studies, P.O. Box 85800, San Diego, CA 92186-5800. Phone: (619) 453-4100, ext. 1559. Fax: (619) 554-0341. E-mail: hope{at}salk.edu.

dagger Present address: Department of Molecular Microbiology, Smith-Kline Beecham Pharmaceuticals, Collegeville, PA 19426.

Mol Cell Biol, July 1998, p. 3889-3899, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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