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Mol Cell Biol, July 1998, p. 3900-3906, Vol. 18, No. 7
Center for Cancer Research and Department of
Biology, Massachusetts Institute of Technology, Cambridge,
Massachusetts 02139
Received 23 December 1997/Returned for modification 18 February
1998/Accepted 3 April 1998
The fibronectin EIIIB exon is alternatively spliced in a
cell-type-specific manner, and TGCATG repeats in the intron downstream of EIIIB have been implicated in this regulation. Analysis of the
intron sequence from several vertebrates shows that the pattern of
repeats in the 3' half of the intron is evolutionarily conserved. Point
mutations in certain highly conserved repeats greatly reduce EIIIB
inclusion, suggesting that a multicomponent complex may recognize
the repeats. Expression of the SR protein SRp40, SRp20, or
ASF/SF2 stimulates EIIIB inclusion. Studies of the interplay between mutations in the repeats and SRp40-stimulated inclusion suggest
that the repeats are recognized in many, if not all, cell types, and
that EIIIB inclusion may be regulated by quantitative changes in
multiple factors.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Alternative Splicing of the Fibronectin EIIIB Exon
Depends on Specific TGCATG Repeats
*
Corresponding author. Mailing address: E17-529, 40 Ames
St., Cambridge, MA 02139-4307. Phone: (617) 253-6421. Fax: (617)
253-3867. E-mail: sharppa{at}mit.edu.
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