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Mol Cell Biol, July 1998, p. 4043-4052, Vol. 18, No. 7
Department of Biology, University of
California, San Diego, La Jolla, California
Received 4 February 1998/Returned for modification 17 March
1998/Accepted 21 April 1998
Phb2p, a homolog of the tumor suppressor protein prohibitin, was
identified in a genetic screen for suppressors of the loss of Mdm12p, a
mitochondrial outer membrane protein required for normal mitochondrial
morphology and inheritance in Saccharomyces cerevisiae.
Phb2p and its homolog, prohibitin (Phb1p), were localized to the
mitochondrial inner membrane and characterized as integral membrane
proteins which depend on each other for their stability. In otherwise
wild-type genetic backgrounds, null mutations in PHB1 and
PHB2 did not confer any obvious phenotypes. However, loss
of function of either PHB1 or PHB2 in cells
with mitochondrial DNA deleted led to altered mitochondrial morphology,
and phb1 or phb2 mutations were synthetically
lethal when combined with a mutation in any of three mitochondrial
inheritance components of the mitochondrial outer membrane, Mdm12p,
Mdm10p, and Mmm1p. These results provide the first evidence of a role
for prohibitin in mitochondrial inheritance and in the regulation of
mitochondrial morphology.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Prohibitin Family Members Interact Genetically with
Mitochondrial Inheritance Components in Saccharomyces
cerevisiae
*
Corresponding author. Mailing address: University of
California, San Diego, Department of Biology, 0347, La Jolla, CA
92093-0347. Phone: (619) 534-4769. Fax: (619) 534-4403. E-mail:
myaffe{at}ucsd.edu.
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