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Mol Cell Biol, August 1998, p. 4455-4462, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme

Tong Ihn Lee, John J. Wyrick, Sang Seok Koh, Ezra G. Jennings, Ellen L. Gadbois, and Richard A. Young*

Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 1 April 1998/Returned for modification 18 May 1998/Accepted 21 May 1998

Activation of protein-encoding genes involves recruitment of an RNA polymerase II holoenzyme to promoters. Since the Srb4 subunit of the holoenzyme is essential for expression of most class II genes and is a target of at least one transcriptional activator, we reasoned that suppressors of a temperature-sensitive mutation in Srb4 would identify other factors generally involved in regulation of gene expression. We report here that MED6 and SRB6, both of which encode essential components of the holoenzyme, are among the dominant suppressors and that the products of these genes interact physically with Srb4. The recessive suppressors include NCB1 (BUR6), NCB2, NOT1, NOT3, NOT5, and CAF1, which encode subunits of NC2 and the Not complex. NC2 and Not proteins are general negative regulators which interact with TATA box binding protein (TBP). Taken together, these results suggest that transcription initiation involves a dynamic balance between activation mediated by specific components of the holoenzyme and repression by multiple TBP-associated regulators.

* Corresponding author. Mailing address: Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142. Phone: (617) 258-5218. Fax: (617) 258-0376. E-mail: young{at}wi.mit.edu.

Mol Cell Biol, August 1998, p. 4455-4462, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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