This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaufman, P. D.
Right arrow Articles by Osley, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaufman, P. D.
Right arrow Articles by Osley, M. A.

 Previous Article  |  Next Article 

Mol Cell Biol, August 1998, p. 4793-4806, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Hir Proteins Are Required for Position-Dependent Gene Silencing in Saccharomyces cerevisiae in the Absence of Chromatin Assembly Factor I

Paul D. Kaufman,1 * Jennifer L. Cohen,1 and Mary Ann Osley2

Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206,1 and Program in Molecular Biology, Sloan Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York, New York, 100212

Received 11 March 1998/Returned for modification 15 April 1998/Accepted 14 May 1998

Chromatin assembly factor I (CAF-I) is a three-subunit histone-binding complex conserved from the yeast Saccharomyces cerevisiae to humans. Yeast cells lacking CAF-I (cacDelta mutants) have defects in heterochromatic gene silencing. In this study, we showed that deletion of HIR genes, which regulate histone gene expression, synergistically reduced gene silencing at telomeres and at the HM loci in cacDelta mutants, although hirDelta mutants had no silencing defects when CAF-I was intact. Therefore, Hir proteins are required for an alternative silencing pathway that becomes important in the absence of CAF-I. Because Hir proteins regulate expression of histone genes, we tested the effects of histone gene deletion and overexpression on telomeric silencing and found that alterations in histone H3 and H4 levels or in core histone stoichiometry reduced silencing in cacDelta mutants but not in wild-type cells. We therefore propose that Hir proteins contribute to silencing indirectly via regulation of histone synthesis. However, deletion of combinations of CAC and HIR genes also affected the growth rate and in some cases caused partial temperature sensitivity, suggesting that global aspects of chromosome function may be affected by the loss of members of both gene families.

* Corresponding author. Mailing address: 351 Donner Laboratory, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-3206. Phone: (510) 486-5846. Fax: (510) 486-6488. E-mail: pdkaufman{at}lbl.gov.

Mol Cell Biol, August 1998, p. 4793-4806, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Anderson, H. E., Wardle, J., Korkut, S. V., Murton, H. E., Lopez-Maury, L., Bahler, J., Whitehall, S. K. (2009). The Fission Yeast HIRA Histone Chaperone Is Required for Promoter Silencing and the Suppression of Cryptic Antisense Transcripts. Mol. Cell. Biol. 29: 5158-5167 [Abstract] [Full Text]  
  • Mersfelder, E. L., Parthun, M. R. (2008). Involvement of Hat1p (Kat1p) Catalytic Activity and Subcellular Localization in Telomeric Silencing. J. Biol. Chem. 283: 29060-29068 [Abstract] [Full Text]  
  • Ye, X., Zerlanko, B., Zhang, R., Somaiah, N., Lipinski, M., Salomoni, P., Adams, P. D. (2007). Definition of pRB- and p53-Dependent and -Independent Steps in HIRA/ASF1a-Mediated Formation of Senescence-Associated Heterochromatin Foci. Mol. Cell. Biol. 27: 2452-2465 [Abstract] [Full Text]  
  • Tanny, J. C., Erdjument-Bromage, H., Tempst, P., Allis, C. D. (2007). Ubiquitylation of histone H2B controls RNA polymerase II transcription elongation independently of histone H3 methylation. Genes Dev. 21: 835-847 [Abstract] [Full Text]  
  • Zhang, R., Chen, W., Adams, P. D. (2007). Molecular Dissection of Formation of Senescence-Associated Heterochromatin Foci. Mol. Cell. Biol. 27: 2343-2358 [Abstract] [Full Text]  
  • Reis, C. C., Campbell, J. L. (2007). Contribution of Trf4/5 and the Nuclear Exosome to Genome Stability Through Regulation of Histone mRNA Levels in Saccharomyces cerevisiae. Genetics 175: 993-1010 [Abstract] [Full Text]  
  • Zhang, R., Liu, S.-t., Chen, W., Bonner, M., Pehrson, J., Yen, T. J., Adams, P. D. (2007). HP1 Proteins Are Essential for a Dynamic Nuclear Response That Rescues the Function of Perturbed Heterochromatin in Primary Human Cells. Mol. Cell. Biol. 27: 949-962 [Abstract] [Full Text]  
  • Workman, J. L. (2006). Nucleosome displacement in transcription. Genes Dev. 20: 2009-2017 [Abstract] [Full Text]  
  • Tamburini, B. A., Carson, J. J., Linger, J. G., Tyler, J. K. (2006). Dominant Mutants of the Saccharomyces cerevisiae ASF1 Histone Chaperone Bypass the Need for CAF-1 in Transcriptional Silencing by Altering Histone and Sir Protein Recruitment. Genetics 173: 599-610 [Abstract] [Full Text]  
  • Greenall, A., Williams, E. S., Martin, K. A., Palmer, J. M., Gray, J., Liu, C., Whitehall, S. K. (2006). Hip3 Interacts with the HIRA Proteins Hip1 and Slm9 and Is Required for Transcriptional Silencing and Accurate Chromosome Segregation. J. Biol. Chem. 281: 8732-8739 [Abstract] [Full Text]  
  • Mersfelder, E. L., Parthun, M. R. (2006). The tale beyond the tail: histone core domain modifications and the regulation of chromatin structure.. Nucleic Acids Res 34: 2653-2662 [Abstract] [Full Text]  
  • Prochasson, P., Florens, L., Swanson, S. K., Washburn, M. P., Workman, J. L. (2005). The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev. 19: 2534-2539 [Abstract] [Full Text]  
  • Sharp, J. A., Rizki, G., Kaufman, P. D. (2005). Regulation of Histone Deposition Proteins Asf1/Hir1 by Multiple DNA Damage Checkpoint Kinases in Saccharomyces cerevisiae. Genetics 171: 885-899 [Abstract] [Full Text]  
  • Phelps-Durr, T. L., Thomas, J., Vahab, P., Timmermans, M. C.P. (2005). Maize rough sheath2 and Its Arabidopsis Orthologue ASYMMETRIC LEAVES1 Interact with HIRA, a Predicted Histone Chaperone, to Maintain knox Gene Silencing and Determinacy during Organogenesis. Plant Cell 17: 2886-2898 [Abstract] [Full Text]  
  • Huang, S., Zhou, H., Katzmann, D., Hochstrasser, M., Atanasova, E., Zhang, Z. (2005). Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc. Natl. Acad. Sci. USA 102: 13410-13415 [Abstract] [Full Text]  
  • Ahmad, A., Kikuchi, H., Takami, Y., Nakayama, T. (2005). Different Roles of N-terminal and C-terminal Halves of HIRA in Transcription Regulation of Cell Cycle-related Genes That Contribute to Control of Vertebrate Cell Growth. J. Biol. Chem. 280: 32090-32100 [Abstract] [Full Text]  
  • Franco, A. A., Lam, W. M., Burgers, P. M., Kaufman, P. D. (2005). Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C. Genes Dev. 19: 1365-1375 [Abstract] [Full Text]  
  • Harkness, T. A. A., Arnason, T. G., Legrand, C., Pisclevich, M. G., Davies, G. F., Turner, E. L. (2005). Contribution of CAF-I to Anaphase-Promoting-Complex-Mediated Mitotic Chromatin Assembly in Saccharomyces cerevisiae. Eukaryot Cell 4: 673-684 [Abstract] [Full Text]  
  • Kamakaka, R. T., Biggins, S. (2005). Histone variants: deviants?. Genes Dev. 19: 295-316 [Abstract] [Full Text]  
  • Glowczewski, L., Waterborg, J. H., Berman, J. G. (2004). Yeast Chromatin Assembly Complex 1 Protein Excludes Nonacetylatable Forms of Histone H4 from Chromatin and the Nucleus. Mol. Cell. Biol. 24: 10180-10192 [Abstract] [Full Text]  
  • Morey, L., Barnes, K., Chen, Y., Fitzgerald-Hayes, M., Baker, R. E. (2004). The Histone Fold Domain of Cse4 Is Sufficient for CEN Targeting and Propagation of Active Centromeres in Budding Yeast. Eukaryot Cell 3: 1533-1543 [Abstract] [Full Text]  
  • Suter, B., Tong, A., Chang, M., Yu, L., Brown, G. W., Boone, C., Rine, J. (2004). The Origin Recognition Complex Links Replication, Sister Chromatid Cohesion and Transcriptional Silencing in Saccharomyces cerevisiae. Genetics 167: 579-591 [Abstract] [Full Text]  
  • Blackwell, C., Martin, K. A., Greenall, A., Pidoux, A., Allshire, R. C., Whitehall, S. K. (2004). The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres. Mol. Cell. Biol. 24: 4309-4320 [Abstract] [Full Text]  
  • FRANCO, A.A., KAUFMAN, P.D. (2004). Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing. Cold Spring Harb Symp Quant Biol 69: 201-208 [Abstract]  
  • PILLUS, L., RINE, J. (2004). SIR1 and the Origin of Epigenetic States in Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol 69: 259-266 [Abstract]  
  • Sharp, J. A., Krawitz, D. C., Gardner, K. A., Fox, C. A., Kaufman, P. D. (2003). The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin. Genes Dev. 17: 2356-2361 [Abstract] [Full Text]  
  • Formosa, T., Ruone, S., Adams, M. D., Olsen, A. E., Eriksson, P., Yu, Y., Rhoades, A. R., Kaufman, P. D., Stillman, D. J. (2002). Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae Cause Dependence on the Hir/Hpc Pathway: Polymerase Passage May Degrade Chromatin Structure. Genetics 162: 1557-1571 [Abstract] [Full Text]  
  • Ner, S. S., Harrington, M. J., Grigliatti, T. A. (2002). A Role for the Drosophila SU(VAR)3-9 Protein in Chromatin Organization at the Histone Gene Cluster and in Suppression of Position-Effect Variegation. Genetics 162: 1763-1774 [Abstract] [Full Text]  
  • Nelson, D. M., Ye, X., Hall, C., Santos, H., Ma, T., Kao, G. D., Yen, T. J., Harper, J. W., Adams, P. D. (2002). Coupling of DNA Synthesis and Histone Synthesis in S Phase Independent of Cyclin/cdk2 Activity. Mol. Cell. Biol. 22: 7459-7472 [Abstract] [Full Text]  
  • Huang, Y. (2002). Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Nucleic Acids Res 30: 1465-1482 [Abstract] [Full Text]  
  • Krawitz, D. C., Kama, T., Kaufman, P. D. (2002). Chromatin Assembly Factor I Mutants Defective for PCNA Binding Require Asf1/Hir Proteins for Silencing. Mol. Cell. Biol. 22: 614-625 [Abstract] [Full Text]  
  • Sharp, J. A., Franco, A. A., Osley, M. A., Kaufman, P. D. (2002). Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev. 16: 85-100 [Abstract] [Full Text]  
  • Nagy, P. L., Griesenbeck, J., Kornberg, R. D., Cleary, M. L. (2001). A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc. Natl. Acad. Sci. USA 10.1073/pnas.221596698v1 [Abstract] [Full Text]  
  • Meijsing, S. H., Ehrenhofer-Murray, A. E. (2001). The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae. Genes Dev. 15: 3169-3182 [Abstract] [Full Text]  
  • Tyler, J. K., Collins, K. A., Prasad-Sinha, J., Amiott, E., Bulger, M., Harte, P. J., Kobayashi, R., Kadonaga, J. T. (2001). Interaction between the Drosophila CAF-1 and ASF1 Chromatin Assembly Factors. Mol. Cell. Biol. 21: 6574-6584 [Abstract] [Full Text]  
  • Sutton, A., Bucaria, J., Osley, M. A., Sternglanz, R. (2001). Yeast ASF1 Protein Is Required for Cell Cycle Regulation of Histone Gene Transcription. Genetics 158: 587-596 [Abstract] [Full Text]  
  • Tchénio, T., Casella, J.-F., Heidmann, T. (2001). A Truncated Form of the Human CAF-1 p150 Subunit Impairs the Maintenance of Transcriptional Gene Silencing in Mammalian Cells. Mol. Cell. Biol. 21: 1953-1961 [Abstract] [Full Text]  
  • Johnston, S. D., Enomoto, S., Schneper, L., McClellan, M. C., Twu, F., Montgomery, N. D., Haney, S. A., Broach, J. R., Berman, J. (2001). CAC3 (MSI1) Suppression of RAS2G19V Is Independent of Chromatin Assembly Factor I and Mediated by NPR1. Mol. Cell. Biol. 21: 1784-1794 [Abstract] [Full Text]  
  • Hall, C., Nelson, D. M., Ye, X., Baker, K., DeCaprio, J. A., Seeholzer, S., Lipinski, M., Adams, P. D. (2001). HIRA, the Human Homologue of Yeast Hir1p and Hir2p, Is a Novel Cyclin-cdk2 Substrate Whose Expression Blocks S-Phase Progression. Mol. Cell. Biol. 21: 1854-1865 [Abstract] [Full Text]  
  • Bernstein, B. E., Tong, J. K., Schreiber, S. L. (2000). Genomewide studies of histone deacetylase function in yeast. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250477697v1 [Abstract] [Full Text]  
  • Kelly, T. J., Qin, S., Gottschling, D. E., Parthun, M. R. (2000). Type B Histone Acetyltransferase Hat1p Participates in Telomeric Silencing. Mol. Cell. Biol. 20: 7051-7058 [Abstract] [Full Text]  
  • Verreault, A. (2000). De novo nucleosome assembly: new pieces in an old puzzle. Genes Dev. 14: 1430-1438 [Full Text]  
  • Enomoto, S., Johnston, S. D., Berman, J. (2000). Identification of a Novel Allele of SIR3 Defective in the Maintenance, but Not the Establishment, of Silencing in Saccharomyces cerevisiae. Genetics 155: 523-538 [Abstract] [Full Text]  
  • Ridgway, P, Almouzni, G (2000). CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair. J. Cell Sci. 113: 2647-2658 [Abstract]  
  • Sun, Z.-W., Hampsey, M. (1999). A General Requirement for the Sin3-Rpd3 Histone Deacetylase Complex in Regulating Silencing in Saccharomyces cerevisiae. Genetics 152: 921-932 [Abstract] [Full Text]  
  • Smith, J. S., Caputo, E., Boeke, J. D. (1999). A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors. Mol. Cell. Biol. 19: 3184-3197 [Abstract] [Full Text]  
  • Huang, H., Hong, J. Y., Burck, C. L., Liebman, S. W. (1999). Host Genes That Affect the Target-Site Distribution of the Yeast Retrotransposon Ty1. Genetics 151: 1393-1407 [Abstract] [Full Text]  
  • Game, J. C., Kaufman, P. D. (1999). Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo. Genetics 151: 485-497 [Abstract] [Full Text]  
  • Qian, Z., Huang, H., Hong, J. Y., Burck, C. L., Johnston, S. D., Berman, J., Carol, A., Liebman, S. W. (1998). Yeast Ty1 Retrotransposition Is Stimulated by a Synergistic Interaction between Mutations in Chromatin Assembly Factor I and Histone Regulatory Proteins. Mol. Cell. Biol. 18: 4783-4792 [Abstract] [Full Text]  
  • Nagy, P. L., Griesenbeck, J., Kornberg, R. D., Cleary, M. L. (2002). A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc. Natl. Acad. Sci. USA 99: 90-94 [Abstract] [Full Text]  
  • Bernstein, B. E., Tong, J. K., Schreiber, S. L. (2000). Genomewide studies of histone deacetylase function in yeast. Proc. Natl. Acad. Sci. USA 97: 13708-13713 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»