This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saffman, E. E.
Right arrow Articles by Lasko, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saffman, E. E.
Right arrow Articles by Lasko, P.

 Previous Article  |  Next Article 

Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Premature Translation of oskar in Oocytes Lacking the RNA-Binding Protein Bicaudal-C

Emma E. Saffman,1 Sylvia Styhler,1 Katherine Rother,1 Weihua Li,1 Stéphane Richard,2 and Paul Lasko1 3 *

Departments of Biology1 and Anatomy and Cell Biology,3 McGill University, Montréal, Québec, Canada H3A 1B1, and Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish Hospital, and Departments of Oncology, Medicine, and Microbiology and Immunology, McGill University, Montréal, Québec, Canada H3T 1E22

Received 29 January 1998/Returned for modification 19 March 1998/Accepted 19 May 1998

Bicaudal-C (Bic-C) is required during Drosophila melanogaster oogenesis for several processes, including anterior-posterior patterning. The gene encodes a protein with five copies of the KH domain, a motif found in a number of RNA-binding proteins. Using antibodies raised against the BIC-C protein, we show that multiple isoforms of the protein exist in ovaries and that the protein, like the RNA, accumulates in the developing oocyte early in oogenesis. BIC-C protein expressed in mammalian cells can bind RNA in vitro, and a point mutation in one of the KH domains that causes a strong Bic-C phenotype weakens this binding. In addition, oskar translation commences prior to posterior localization of oskar RNA in Bic-C- oocytes, indicating that Bic-C may regulate oskar translation during oogenesis.

* Corresponding author. Mailing address: Department of Biology, McGill University, 1205 Ave. Docteur Penfield, Montréal, Québec, Canada H3A 1B1. Phone: (514) 398 6721. Fax: (514) 398 8051. E-mail: Paul_Lasko{at}maclan.mcgill.ca.

Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maisonneuve, C., Guilleret, I., Vick, P., Weber, T., Andre, P., Beyer, T., Blum, M., Constam, D. B. (2009). Bicaudal C, a novel regulator of Dvl signaling abutting RNA-processing bodies, controls cilia orientation and leftward flow. Development 136: 3019-3030 [Abstract] [Full Text]  
  • Benoit, P., Papin, C., Kwak, J. E., Wickens, M., Simonelig, M. (2008). PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development 135: 1969-1979 [Abstract] [Full Text]  
  • Cui, J., Sackton, K. L., Horner, V. L., Kumar, K. E., Wolfner, M. F. (2008). Wispy, the Drosophila Homolog of GLD-2, Is Required During Oogenesis and Egg Activation. Genetics 178: 2017-2029 [Abstract] [Full Text]  
  • Shapiro, R. S., Anderson, K. V. (2006). Drosophila Ik2, a member of the I{kappa}B kinase family, is required for mRNA localization during oogenesis. Development 133: 1467-1475 [Abstract] [Full Text]  
  • Brown, J. H., Bihoreau, M.-T., Hoffmann, S., Kranzlin, B., Tychinskaya, I., Obermuller, N., Podlich, D., Boehn, S. N., Kaisaki, P. J., Megel, N., Danoy, P., Copley, R. R., Broxholme, J., Witzgall, R., Lathrop, M., Gretz, N., Gauguier, D. (2005). Missense Mutation in Sterile {alpha} Motif of Novel Protein SamCystin is Associated with Polycystic Kidney Disease in (cy/+) Rat. J. Am. Soc. Nephrol. 16: 3517-3526 [Abstract] [Full Text]  
  • Lasko, P. (2003). Cup-ling oskar RNA localization and translational control. JCB 163: 1189-1191 [Abstract] [Full Text]  
  • Wilhelm, J. E., Hilton, M., Amos, Q., Henzel, W. J. (2003). Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. JCB 163: 1197-1204 [Abstract] [Full Text]  
  • Guay-Woodford, L. M. (2003). Murine models of polycystic kidney disease: molecular and therapeutic insights. Am. J. Physiol. Renal Physiol. 285: F1034-F1049 [Abstract] [Full Text]  
  • Lasko, P. (2003). Gene Regulation at the RNA Layer: RNA Binding Proteins in Intercellular Signaling Networks. Sci Signal 2003: re6-re6 [Abstract] [Full Text]  
  • Sinka, R., Jankovics, F., Somogyi, K., Szlanka, T., Lukacsovich, T., Erdelyi, M. (2003). poirot, a new regulatory gene of Drosophila oskar acts at the level of the short Oskar protein isoform. Development 129: 3469-3478 [Abstract] [Full Text]  
  • Castagnetti, S., Ephrussi, A. (2003). Orb and a long poly(A) tail are required for efficient oskar translation at the posterior pole of the Drosophila oocyte. Development 130: 835-843 [Abstract] [Full Text]  
  • Mansfield, J. H., Wilhelm, J. E., Hazelrigg, T. (2002). Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway. Development 129: 197-209 [Abstract] [Full Text]  
  • Volpe, A. M., Horowitz, H., Grafer, C. M., Jackson, S. M., Berg, C. A. (2001). Drosophila rhino Encodes a Female-Specific Chromo-domain Protein That Affects Chromosome Structure and Egg Polarity. Genetics 159: 1117-1134 [Abstract] [Full Text]  
  • Harris, A. N., Macdonald, P. M. (2001). aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development 128: 2823-2832 [Abstract] [Full Text]  
  • GEBAUER, F., OSTARECK, D.H., OSTARECK-LEDERER, A., GRSKOVIC, M., HENTZE, M.W. (2001). Translational Control of 15-Lipoxygenase and msl-2 mRNAs: Single Regulators or Corepressor Assemblies?. Cold Spring Harb Symp Quant Biol 66: 329-336 [Abstract]  
  • Wessely, O., De Robertis, E. M. (2000). The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Development 127: 2053-2062 [Abstract]  
  • Castagnetti, S, Hentze, M., Ephrussi, A, Gebauer, F (2000). Control of oskar mRNA translation by Bruno in a novel cell-free system from Drosophila ovaries. Development 127: 1063-1068 [Abstract]  
  • LASKO, P. (1999). RNA sorting in Drosophila oocytes and embryos. FASEB J. 13: 421-433 [Abstract] [Full Text]  
  • Lie, Y., Macdonald, P. (1999). Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. Development 126: 1129-1138 [Abstract]  
  • Di Fruscio, M., Chen, T., Bonyadi, S., Lasko, P., Richard, S. (1998). The Identification of Two Drosophila K Homology Domain Proteins. KEP1 AND SAM ARE MEMBERS OF THE Sam68 FAMILY OF GSG DOMAIN PROTEINS. J. Biol. Chem. 273: 30122-30130 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»