Previous Article | Next Article 
Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Premature Translation of oskar in
Oocytes Lacking the RNA-Binding Protein Bicaudal-C
Emma E.
Saffman,1
Sylvia
Styhler,1
Katherine
Rother,1
Weihua
Li,1
Stéphane
Richard,2 and
Paul
Lasko1 3 *
Departments of
Biology1 and
Anatomy and Cell
Biology,3 McGill University, Montréal,
Québec, Canada H3A 1B1, and
Terry Fox Molecular Oncology
Group, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish Hospital, and Departments of Oncology, Medicine, and
Microbiology and Immunology, McGill University, Montréal,
Québec, Canada H3T 1E22
Received 29 January 1998/Returned for modification 19 March
1998/Accepted 19 May 1998
Bicaudal-C (Bic-C) is required during
Drosophila melanogaster oogenesis for several processes,
including anterior-posterior patterning. The gene encodes a protein
with five copies of the KH domain, a motif found in a number of
RNA-binding proteins. Using antibodies raised against the BIC-C
protein, we show that multiple isoforms of the protein exist in ovaries
and that the protein, like the RNA, accumulates in the developing
oocyte early in oogenesis. BIC-C protein expressed in mammalian cells
can bind RNA in vitro, and a point mutation in one of the KH domains
that causes a strong Bic-C phenotype weakens this binding.
In addition, oskar translation commences prior to posterior
localization of oskar RNA in
Bic-C
oocytes, indicating that
Bic-C may regulate oskar translation during
oogenesis.
*
Corresponding author. Mailing address: Department of
Biology, McGill University, 1205 Ave. Docteur Penfield, Montréal,
Québec, Canada H3A 1B1. Phone: (514) 398 6721. Fax: (514) 398 8051. E-mail: Paul_Lasko{at}maclan.mcgill.ca.
Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Maisonneuve, C., Guilleret, I., Vick, P., Weber, T., Andre, P., Beyer, T., Blum, M., Constam, D. B.
(2009). Bicaudal C, a novel regulator of Dvl signaling abutting RNA-processing bodies, controls cilia orientation and leftward flow. Development
136: 3019-3030
[Abstract]
[Full Text]
-
Benoit, P., Papin, C., Kwak, J. E., Wickens, M., Simonelig, M.
(2008). PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development
135: 1969-1979
[Abstract]
[Full Text]
-
Cui, J., Sackton, K. L., Horner, V. L., Kumar, K. E., Wolfner, M. F.
(2008). Wispy, the Drosophila Homolog of GLD-2, Is Required During Oogenesis and Egg Activation. Genetics
178: 2017-2029
[Abstract]
[Full Text]
-
Shapiro, R. S., Anderson, K. V.
(2006). Drosophila Ik2, a member of the I{kappa}B kinase family, is required for mRNA localization during oogenesis. Development
133: 1467-1475
[Abstract]
[Full Text]
-
Brown, J. H., Bihoreau, M.-T., Hoffmann, S., Kranzlin, B., Tychinskaya, I., Obermuller, N., Podlich, D., Boehn, S. N., Kaisaki, P. J., Megel, N., Danoy, P., Copley, R. R., Broxholme, J., Witzgall, R., Lathrop, M., Gretz, N., Gauguier, D.
(2005). Missense Mutation in Sterile {alpha} Motif of Novel Protein SamCystin is Associated with Polycystic Kidney Disease in (cy/+) Rat. J. Am. Soc. Nephrol.
16: 3517-3526
[Abstract]
[Full Text]
-
Lasko, P.
(2003). Cup-ling oskar RNA localization and translational control. JCB
163: 1189-1191
[Abstract]
[Full Text]
-
Wilhelm, J. E., Hilton, M., Amos, Q., Henzel, W. J.
(2003). Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. JCB
163: 1197-1204
[Abstract]
[Full Text]
-
Guay-Woodford, L. M.
(2003). Murine models of polycystic kidney disease: molecular and therapeutic insights. Am. J. Physiol. Renal Physiol.
285: F1034-F1049
[Abstract]
[Full Text]
-
Lasko, P.
(2003). Gene Regulation at the RNA Layer: RNA Binding Proteins in Intercellular Signaling Networks. Sci Signal
2003: re6-re6
[Abstract]
[Full Text]
-
Sinka, R., Jankovics, F., Somogyi, K., Szlanka, T., Lukacsovich, T., Erdelyi, M.
(2003). poirot, a new regulatory gene of Drosophila oskar acts at the level of the short Oskar protein isoform. Development
129: 3469-3478
[Abstract]
[Full Text]
-
Castagnetti, S., Ephrussi, A.
(2003). Orb and a long poly(A) tail are required for efficient oskar translation at the posterior pole of the Drosophila oocyte. Development
130: 835-843
[Abstract]
[Full Text]
-
Mansfield, J. H., Wilhelm, J. E., Hazelrigg, T.
(2002). Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway. Development
129: 197-209
[Abstract]
[Full Text]
-
Volpe, A. M., Horowitz, H., Grafer, C. M., Jackson, S. M., Berg, C. A.
(2001). Drosophila rhino Encodes a Female-Specific Chromo-domain Protein That Affects Chromosome Structure and Egg Polarity. Genetics
159: 1117-1134
[Abstract]
[Full Text]
-
Harris, A. N., Macdonald, P. M.
(2001). aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development
128: 2823-2832
[Abstract]
[Full Text]
-
GEBAUER, F., OSTARECK, D.H., OSTARECK-LEDERER, A., GRSKOVIC, M., HENTZE, M.W.
(2001). Translational Control of 15-Lipoxygenase and msl-2 mRNAs: Single Regulators or Corepressor Assemblies?. Cold Spring Harb Symp Quant Biol
66: 329-336
[Abstract]
-
Wessely, O., De Robertis, E. M.
(2000). The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Development
127: 2053-2062
[Abstract]
-
Castagnetti, S, Hentze, M., Ephrussi, A, Gebauer, F
(2000). Control of oskar mRNA translation by Bruno in a novel cell-free system from Drosophila ovaries. Development
127: 1063-1068
[Abstract]
-
LASKO, P.
(1999). RNA sorting in Drosophila oocytes and embryos. FASEB J.
13: 421-433
[Abstract]
[Full Text]
-
Lie, Y., Macdonald, P.
(1999). Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. Development
126: 1129-1138
[Abstract]
-
Di Fruscio, M., Chen, T., Bonyadi, S., Lasko, P., Richard, S.
(1998). The Identification of Two Drosophila K Homology Domain Proteins. KEP1 AND SAM ARE MEMBERS OF THE Sam68 FAMILY OF GSG DOMAIN PROTEINS. J. Biol. Chem.
273: 30122-30130
[Abstract]
[Full Text]