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Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Premature Translation of oskar in Oocytes Lacking the RNA-Binding Protein Bicaudal-C

Emma E. Saffman,1 Sylvia Styhler,1 Katherine Rother,1 Weihua Li,1 Stéphane Richard,2 and Paul Lasko1 3 *

Departments of Biology1 and Anatomy and Cell Biology,3 McGill University, Montréal, Québec, Canada H3A 1B1, and Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish Hospital, and Departments of Oncology, Medicine, and Microbiology and Immunology, McGill University, Montréal, Québec, Canada H3T 1E22

Received 29 January 1998/Returned for modification 19 March 1998/Accepted 19 May 1998

Bicaudal-C (Bic-C) is required during Drosophila melanogaster oogenesis for several processes, including anterior-posterior patterning. The gene encodes a protein with five copies of the KH domain, a motif found in a number of RNA-binding proteins. Using antibodies raised against the BIC-C protein, we show that multiple isoforms of the protein exist in ovaries and that the protein, like the RNA, accumulates in the developing oocyte early in oogenesis. BIC-C protein expressed in mammalian cells can bind RNA in vitro, and a point mutation in one of the KH domains that causes a strong Bic-C phenotype weakens this binding. In addition, oskar translation commences prior to posterior localization of oskar RNA in Bic-C- oocytes, indicating that Bic-C may regulate oskar translation during oogenesis.

* Corresponding author. Mailing address: Department of Biology, McGill University, 1205 Ave. Docteur Penfield, Montréal, Québec, Canada H3A 1B1. Phone: (514) 398 6721. Fax: (514) 398 8051. E-mail: Paul_Lasko{at}maclan.mcgill.ca.

Mol Cell Biol, August 1998, p. 4855-4862, Vol. 18, No. 8
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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