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Molecular and Cellular Biology, September 1998, p. 4971-4976, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Yeast Coactivator MBF1 Mediates GCN4-Dependent Transcriptional Activation

Ken-ichi Takemaru,1 Satoshi Harashima,2 Hitoshi Ueda,1 and Susumu Hirose1,*

Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540,1 and Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565,2 Japan

Received 2 April 1998/Returned for modification 18 May 1998/Accepted 29 May 1998

Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.

* Corresponding author. Mailing address: Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan. Phone: 81-559-81-6771. Fax: 81-559-81-6776. E-mail: shirose{at}lab.nig.ac.jp.

Molecular and Cellular Biology, September 1998, p. 4971-4976, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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