Molecular and Cellular Biology, September 1998, p. 4971-4976, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Department of Developmental Genetics,
Received 2 April 1998/Returned for modification 18 May
1998/Accepted 29 May 1998
Transcriptional coactivators play a crucial role in gene expression
by communicating between regulatory factors and the basal transcription
machinery. The coactivator multiprotein bridging factor 1 (MBF1) was
originally identified as a bridging molecule that connects the
Drosophila nuclear receptor FTZ-F1 and TATA-binding protein
(TBP). The MBF1 sequence is highly conserved across species from
Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4
and TBP. These findings indicate that the coactivator MBF1 functions by
recruiting TBP to promoters where DNA-binding regulators are bound.
*
Corresponding author. Mailing address: Department of
Developmental Genetics, National Institute of Genetics, Mishima,
Shizuoka-ken 411-8540, Japan. Phone: 81-559-81-6771. Fax:
81-559-81-6776. E-mail: shirose{at}lab.nig.ac.jp.
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