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Molecular and Cellular Biology, September 1998, p. 5062-5072, Vol. 18, No. 9
Department of Molecular and Cell Biology,
University of California at Berkeley, Berkeley, California
94720,1 and
Centre de Recherche de
Biochimie Macromoléculaire, CNRS, F-34033 Montpellier, Cedex
1, France2
Received 6 April 1998/Returned for modification 20 May
1998/Accepted 1 June 1998
mRNA in the yeast Saccharomyces cerevisiae is primarily
degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8
(YJL124c) gene, initially identified as a suppressor
mutation of a poly(A)-binding protein (PAB1) gene deletion,
stabilizes the mRNA cap structure. Specifically, we find that the
spb8-2 mutation results in the accumulation of capped,
poly(A)-deficient mRNAs. The presence of this mutation also allows for
the detection of mRNA species trimmed from the 3' end. These data show
that this Sm-like protein family member is involved in the process of
mRNA decapping, and they provide an example of 3'-5' mRNA degradation
intermediates in yeast.
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Capped mRNA Degradation Intermediates
Accumulate in the Yeast spb8-2 Mutant
*
Corresponding author. Mailing address: Department of
Molecular and Cell Biology, University of California at Berkeley,
Berkeley, CA 94720. Phone: (510) 643-7698. Fax: (510) 643-5035. E-mail: asachs{at}uclink4.berkeley.edu.
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