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Molecular and Cellular Biology, September 1998, p. 5062-5072, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant

Ronald Boeck,1 Bruno Lapeyre,2 Christine E. Brown,1 and Alan B. Sachs1,*

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720,1 and Centre de Recherche de Biochimie Macromoléculaire, CNRS, F-34033 Montpellier, Cedex 1, France2

Received 6 April 1998/Returned for modification 20 May 1998/Accepted 1 June 1998

mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720. Phone: (510) 643-7698. Fax: (510) 643-5035. E-mail: asachs{at}uclink4.berkeley.edu.

Molecular and Cellular Biology, September 1998, p. 5062-5072, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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