This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimomura, T.
Right arrow Articles by Sugimoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimomura, T.
Right arrow Articles by Sugimoto, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1998, p. 5485-5491, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Functional and Physical Interaction between Rad24 and Rfc5 in the Yeast Checkpoint Pathways

Toshiyasu Shimomura, Seiko Ando, Kunihiro Matsumoto,* and Katsunori Sugimoto

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-0814, Japan

Received 24 March 1998/Returned for modification 27 April 1998/Accepted 22 June 1998

The RFC5 gene encodes a small subunit of replication factor C (RFC) complex in Saccharomyces cerevisiae and has been shown to be required for the checkpoints which respond to replication block and DNA damage. Here we describe the isolation of RAD24, known to play a role in the DNA damage checkpoint, as a dosage-dependent suppressor of rfc5-1. RAD24 overexpression suppresses the sensitivity of rfc5-1 cells to DNA-damaging agents and the defect in DNA damage-induced Rad53 phosphorylation. Rad24, like Rfc5, is required for the regulation of Rad53 phosphorylation in response to DNA damage. The Rad24 protein, which is structurally related to the RFC subunits, interacts physically with RFC subunits Rfc2 and Rfc5 and cosediments with Rfc5. Although the rad24Delta mutation alone does not cause a defect in the replication block checkpoint, it does enhance the defect in rfc5-1 mutants. Furthermore, overexpression of RAD24 suppresses the rfc5-1 defect in the replication block checkpoint. Taken together, our results demonstrate a physical and functional interaction between Rad24 and Rfc5 in the checkpoint pathways.

* Corresponding author. Mailing address: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-0814, Japan. Phone: 81-52-789-2593. Fax: 81-52-789-2589. E-mail: g44177a{at}nucc.cc.nagoya-u.ac.up.

Molecular and Cellular Biology, September 1998, p. 5485-5491, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Celic, I., Verreault, A., Boeke, J. D. (2008). Histone H3 K56 Hyperacetylation Perturbs Replisomes and Causes DNA Damage. Genetics 179: 1769-1784 [Abstract] [Full Text]  
  • Yao, N. Y., Johnson, A., Bowman, G. D., Kuriyan, J., O'Donnell, M. (2006). Mechanism of Proliferating Cell Nuclear Antigen Clamp Opening by Replication Factor C. J. Biol. Chem. 281: 17528-17539 [Abstract] [Full Text]  
  • Kim, J., Robertson, K., Mylonas, K. J. L., Gray, F. C., Charapitsa, I., MacNeill, S. A. (2005). Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast. Nucleic Acids Res 33: 4078-4089 [Abstract] [Full Text]  
  • Munshi, A., Cannella, D., Brickner, H., Salles-Passador, I., Podust, V., Fotedar, R., Fotedar, A. (2003). Cell Cycle-dependent Phosphorylation of the Large Subunit of Replication Factor C (RF-C) Leads to Its Dissociation from the RF-C Complex. J. Biol. Chem. 278: 48467-48473 [Abstract] [Full Text]  
  • Jones, R. E., Chapman, J. R., Puligilla, C., Murray, J. M., Car, A. M., Ford, C. C., Lindsay, H. D. (2003). XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus. Mol. Biol. Cell 14: 3898-3910 [Abstract] [Full Text]  
  • Kenna, M. A., Skibbens, R. V. (2003). Mechanical Link between Cohesion Establishment and DNA Replication: Ctf7p/Eco1p, a Cohesion Establishment Factor, Associates with Three Different Replication Factor C Complexes. Mol. Cell. Biol. 23: 2999-3007 [Abstract] [Full Text]  
  • Majka, J., Burgers, P. M. J. (2003). Yeast Rad17/Mec3/Ddc1: A sliding clamp for the DNA damage checkpoint. Proc. Natl. Acad. Sci. USA 100: 2249-2254 [Abstract] [Full Text]  
  • IJpma, A. S., Greider, C. W. (2003). Short Telomeres Induce a DNA Damage Response in Saccharomyces cerevisiae. Mol. Biol. Cell 14: 987-1001 [Abstract] [Full Text]  
  • Stokes, M. P., Van Hatten, R., Lindsay, H. D., Michael, W. M. (2002). DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts. JCB 158: 863-872 [Abstract] [Full Text]  
  • Unsal-Kacmaz, K., Makhov, A. M., Griffith, J. D., Sancar, A. (2002). Preferential binding of ATR protein to UV-damaged DNA. Proc. Natl. Acad. Sci. USA 99: 6673-6678 [Abstract] [Full Text]  
  • Griffith, J. D., Lindsey-Boltz, L. A., Sancar, A. (2002). Structures of the Human Rad17-Replication Factor C and Checkpoint Rad 9-1-1 Complexes Visualized by Glycerol Spray/Low Voltage Microscopy. J. Biol. Chem. 277: 15233-15236 [Abstract] [Full Text]  
  • Myung, K., Kolodner, R. D. (2002). Inaugural Article: Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 99: 4500-4507 [Abstract] [Full Text]  
  • Kondo, T., Wakayama, T., Naiki, T., Matsumoto, K., Sugimoto, K. (2001). Recruitment of Mec1 and Ddc1 Checkpoint Proteins to Double-Strand Breaks Through Distinct Mechanisms. Science 294: 867-870 [Abstract] [Full Text]  
  • Wang, X., Wang, L., Callister, M. D., Putnam, J. B., Mao, L., Li, L. (2001). Human Rad17 Is Phosphorylated upon DNA Damage and also Overexpressed in Primary Non-Small Cell Lung Cancer Tissues. Cancer Res. 61: 7417-7421 [Abstract] [Full Text]  
  • Lindsey-Boltz, L. A., Bermudez, V. P., Hurwitz, J., Sancar, A. (2001). Purification and characterization of human DNA damage checkpoint Rad complexes. Proc. Natl. Acad. Sci. USA 98: 11236-11241 [Abstract] [Full Text]  
  • Naiki, T., Kondo, T., Nakada, D., Matsumoto, K., Sugimoto, K. (2001). Chl12 (Ctf18) Forms a Novel Replication Factor C-Related Complex and Functions Redundantly with Rad24 in the DNA Replication Checkpoint Pathway. Mol. Cell. Biol. 21: 5838-5845 [Abstract] [Full Text]  
  • Krause, S. A., Loupart, M.-L., Vass, S., Schoenfelder, S., Harrison, S., Heck, M. M. S. (2001). Loss of Cell Cycle Checkpoint Control in Drosophila Rfc4 Mutants. Mol. Cell. Biol. 21: 5156-5168 [Abstract] [Full Text]  
  • Kim, H.-S., Brill, S. J. (2001). Rfc4 Interacts with Rpal and Is Required for Both DNA Replication and DNA Damage Checkpoints in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 3725-3737 [Abstract] [Full Text]  
  • Kai, M., Tanaka, H., Wang, T. S.-F. (2001). Fission Yeast Rad17 Associates with Chromatin in Response to Aberrant Genomic Structures. Mol. Cell. Biol. 21: 3289-3301 [Abstract] [Full Text]  
  • Hanna, J. S., Kroll, E. S., Lundblad, V., Spencer, F. A. (2001). Saccharomyces cerevisiae CTF18 and CTF4 Are Required for Sister Chromatid Cohesion. Mol. Cell. Biol. 21: 3144-3158 [Abstract] [Full Text]  
  • Wakayama, T., Kondo, T., Ando, S., Matsumoto, K., Sugimoto, K. (2001). Pie1, a Protein Interacting with Mec1, Controls Cell Growth and Checkpoint Responses in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 755-764 [Abstract] [Full Text]  
  • Naiki, T., Shimomura, T., Kondo, T., Matsumoto, K., Sugimoto, K. (2000). Rfc5, in Cooperation with Rad24, Controls DNA Damage Checkpoints throughout the Cell Cycle in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 5888-5896 [Abstract] [Full Text]  
  • Gomes, X. V., Gary, S. L., Burgers, P. M. J. (2000). Overproduction in Escherichia coli and Characterization of Yeast Replication Factor C Lacking the Ligase Homology Domain. J. Biol. Chem. 275: 14541-14549 [Abstract] [Full Text]  
  • Wang, Y., Cortez, D., Yazdi, P., Neff, N., Elledge, S. J., Qin, J. (2000). BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14: 927-939 [Abstract] [Full Text]  
  • Caspari, T., Dahlen, M., Kanter-Smoler, G., Lindsay, H. D., Hofmann, K., Papadimitriou, K., Sunnerhagen, P., Carr, A. M. (2000). Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. Mol. Cell. Biol. 20: 1254-1262 [Abstract] [Full Text]  
  • Griffiths, D, Uchiyama, M, Nurse, P, Wang, T. (2000). A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints. J. Cell Sci. 113: 1075-1088 [Abstract]  
  • WEINERT, T., LITTLE, E., SHANKS, L., ADMIRE, A., GARDNER, R., PUTNAM, C., MICHELSON, R., NYBERG, K., SUNDARESHAN, P. (2000). Details and Concerns Regarding the G2/M DNA Damage Checkpoint in Budding Yeast. Cold Spring Harb Symp Quant Biol 65: 433-442 [Abstract]  
  • WEISS, R.S., LEDER, P., ENOCH, T. (2000). A Conserved Role for the Hus1 Checkpoint Protein in Eukaryotic Genome Maintenance. Cold Spring Harb Symp Quant Biol 65: 457-466 [Abstract]  
  • Amin, N. S., Tuffo, K. M., Holm, C. (1999). Dominant Mutations in Three Different Subunits of Replication Factor C Suppress Replication Defects in Yeast PCNA Mutants. Genetics 153: 1617-1628 [Abstract] [Full Text]  
  • Shimada, M., Okuzaki, D., Tanaka, S., Tougan, T., Tamai, K. K., Shimoda, C., Nojima, H. (1999). Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints. Mol. Biol. Cell 10: 3991-4003 [Abstract] [Full Text]  
  • Kondo, T., Matsumoto, K., Sugimoto, K. (1999). Role of a Complex Containing Rad17, Mec3, and Ddc1 in the Yeast DNA Damage Checkpoint Pathway. Mol. Cell. Biol. 19: 1136-1143 [Abstract] [Full Text]  
  • Xia, Z., Morales, J. C., Dunphy, W. G., Carpenter, P. B. (2001). Negative Cell Cycle Regulation and DNA Damage-inducible Phosphorylation of the BRCT Protein 53BP1. J. Biol. Chem. 276: 2708-2718 [Abstract] [Full Text]  
  • Schmidt, S. L. G., Pautz, A. L., Burgers, P. M. J. (2001). ATP Utilization by Yeast Replication Factor C. IV. RFC ATP-BINDING MUTANTS SHOW DEFECTS IN DNA REPLICATION, DNA REPAIR, AND CHECKPOINT REGULATION. J. Biol. Chem. 276: 34792-34800 [Abstract] [Full Text]  
  • Zhang, H., Zhu, Z., Vidanes, G., Mbangkollo, D., Liu, Y., Siede, W. (2001). Characterization of DNA Damage-stimulated Self-interaction of Saccharomyces cerevisiae Checkpoint Protein Rad17p. J. Biol. Chem. 276: 26715-26723 [Abstract] [Full Text]  
  • Myung, K., Kolodner, R. D. (2002). Inaugural Article: Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 99: 4500-4507 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»