This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lorain, S.
Right arrow Articles by Lipinski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lorain, S.
Right arrow Articles by Lipinski, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1998, p. 5546-5556, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Core Histones and HIRIP3, a Novel Histone-Binding Protein, Directly Interact with WD Repeat Protein HIRA

Stéphanie Lorain,1 Jean-Pierre Quivy,2 Frédérique Monier-Gavelle,1 Christine Scamps,1 Yann Lécluse,1 Geneviève Almouzni,2 and Marc Lipinski1,*

Biologie des Tumeurs Humaines, CNRS UMR 1598, Institut Gustave Roussy, Villejuif,1 and Dynamique de la Chromatine, CNRS UMR 144, Institut Curie, Section de Recherche, Paris,2 France

Received 23 January 1998/Returned for modification 5 March 1998/Accepted 10 June 1998

The human HIRA gene has been named after Hir1p and Hir2p, two corepressors which together appear to act on chromatin structure to control gene transcription in Saccharomyces cerevisiae. HIRA homologs are expressed in a regulated fashion during mouse and chicken embryogenesis, and the human gene is a major candidate for the DiGeorge syndrome and related developmental disorders caused by a reduction to single dose of a fragment of chromosome 22q. Western blot analysis and double-immunofluorescence experiments using a specific antiserum revealed a primary nuclear localization of HIRA. Similar to Hir1p, HIRA contains seven amino-terminal WD repeats and probably functions as part of a multiprotein complex. HIRA and core histone H2B were found to physically interact in a yeast double-hybrid protein interaction trap, in GST pull-down assays, and in coimmunoprecipitation experiments performed from cellular extracts. In vitro, HIRA also interacted with core histone H4. H2B- and H4-binding domains were overlapping but distinguishable in the carboxy-terminal region of HIRA, and the region for HIRA interaction was mapped to the amino-terminal tail of H2B and the second alpha  helix of H4. HIRIP3 (HIRA-interacting protein 3) is a novel gene product that was identified from its HIRA-binding properties in the yeast protein interaction trap. In vitro, HIRIP3 directly interacted with HIRA but also with core histones H2B and H3, suggesting that a HIRA-HIRIP3-containing complex could function in some aspects of chromatin and histone metabolism. Insufficient production of HIRA, which we report elsewhere interacts with homeodomain-containing DNA-binding factors during mammalian embryogenesis, could perturb the stoichiometric assembly of multimolecular complexes required for normal embryonic development.

* Corresponding author. Mailing address: Laboratoire de Biologie des Tumeurs Humaines, CNRS UMR 1598, Rue Camille Desmoulins, Institut Gustave Roussy, 94805 Villejuif Cedex, France. Phone: 33 1 42 11 49 17. Fax: 33 1 42 11 54 94. E-mail: lipinski{at}igr.fr.

Molecular and Cellular Biology, September 1998, p. 5546-5556, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ye, X., Zerlanko, B., Zhang, R., Somaiah, N., Lipinski, M., Salomoni, P., Adams, P. D. (2007). Definition of pRB- and p53-Dependent and -Independent Steps in HIRA/ASF1a-Mediated Formation of Senescence-Associated Heterochromatin Foci. Mol. Cell. Biol. 27: 2452-2465 [Abstract] [Full Text]  
  • Tie, F., Stratton, C. A., Kurzhals, R. L., Harte, P. J. (2007). The N Terminus of Drosophila ESC Binds Directly to Histone H3 and Is Required for E(Z)-Dependent Trimethylation of H3 Lysine 27. Mol. Cell. Biol. 27: 2014-2026 [Abstract] [Full Text]  
  • Takami, Y., Ono, T., Fukagawa, T., Shibahara, K.-i., Nakayama, T. (2007). Essential Role of Chromatin Assembly Factor-1-mediated Rapid Nucleosome Assembly for DNA Replication and Cell Division in Vertebrate Cells. Mol. Biol. Cell 18: 129-141 [Abstract] [Full Text]  
  • Szodoray, P., Alex, P., Frank, M. B., Turner, M., Turner, S., Knowlton, N., Cadwell, C., Dozmorov, I., Tang, Y., Wilson, P. C., Jonsson, R., Centola, M. (2006). A genome-scale assessment of peripheral blood B-cell molecular homeostasis in patients with rheumatoid arthritis. Rheumatology (Oxford) 45: 1466-1476 [Abstract] [Full Text]  
  • Prochasson, P., Florens, L., Swanson, S. K., Washburn, M. P., Workman, J. L. (2005). The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev. 19: 2534-2539 [Abstract] [Full Text]  
  • Ahmad, A., Kikuchi, H., Takami, Y., Nakayama, T. (2005). Different Roles of N-terminal and C-terminal Halves of HIRA in Transcription Regulation of Cell Cycle-related Genes That Contribute to Control of Vertebrate Cell Growth. J. Biol. Chem. 280: 32090-32100 [Abstract] [Full Text]  
  • Jayaramaiah Raja, S., Renkawitz-Pohl, R. (2005). Replacement by Drosophila melanogaster Protamines and Mst77F of Histones during Chromatin Condensation in Late Spermatids and Role of Sesame in the Removal of These Proteins from the Male Pronucleus. Mol. Cell. Biol. 25: 6165-6177 [Abstract] [Full Text]  
  • Blackwell, C., Martin, K. A., Greenall, A., Pidoux, A., Allshire, R. C., Whitehall, S. K. (2004). The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres. Mol. Cell. Biol. 24: 4309-4320 [Abstract] [Full Text]  
  • Nabatiyan, A., Krude, T. (2004). Silencing of Chromatin Assembly Factor 1 in Human Cells Leads to Cell Death and Loss of Chromatin Assembly during DNA Synthesis. Mol. Cell. Biol. 24: 2853-2862 [Abstract] [Full Text]  
  • Palmer, H. G., Sanchez-Carbayo, M., Ordonez-Moran, P., Larriba, M. J., Cordon-Cardo, C., Munoz, A. (2003). Genetic Signatures of Differentiation Induced by 1{alpha},25-Dihydroxyvitamin D3 in Human Colon Cancer Cells. Cancer Res. 63: 7799-7806 [Abstract] [Full Text]  
  • Hoek, M., Stillman, B. (2003). Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo. Proc. Natl. Acad. Sci. USA 100: 12183-12188 [Abstract] [Full Text]  
  • Ganesh, S., Tsurutani, N., Suzuki, T., Ueda, K., Agarwala, K. L., Osada, H., Delgado-Escueta, A. V., Yamakawa, K. (2003). The Lafora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a NifU-like domain. Hum Mol Genet 12: 2359-2368 [Abstract] [Full Text]  
  • Formosa, T., Ruone, S., Adams, M. D., Olsen, A. E., Eriksson, P., Yu, Y., Rhoades, A. R., Kaufman, P. D., Stillman, D. J. (2002). Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae Cause Dependence on the Hir/Hpc Pathway: Polymerase Passage May Degrade Chromatin Structure. Genetics 162: 1557-1571 [Abstract] [Full Text]  
  • Nelson, D. M., Ye, X., Hall, C., Santos, H., Ma, T., Kao, G. D., Yen, T. J., Harper, J. W., Adams, P. D. (2002). Coupling of DNA Synthesis and Histone Synthesis in S Phase Independent of Cyclin/cdk2 Activity. Mol. Cell. Biol. 22: 7459-7472 [Abstract] [Full Text]  
  • Roberts, C., Sutherland, H. F., Farmer, H., Kimber, W., Halford, S., Carey, A., Brickman, J. M., Wynshaw-Boris, A., Scambler, P. J. (2002). Targeted Mutagenesis of the Hira Gene Results in Gastrulation Defects and Patterning Abnormalities of Mesoendodermal Derivatives Prior to Early Embryonic Lethality. Mol. Cell. Biol. 22: 2318-2328 [Abstract] [Full Text]  
  • Sharp, J. A., Franco, A. A., Osley, M. A., Kaufman, P. D. (2002). Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev. 16: 85-100 [Abstract] [Full Text]  
  • Sutton, A., Bucaria, J., Osley, M. A., Sternglanz, R. (2001). Yeast ASF1 Protein Is Required for Cell Cycle Regulation of Histone Gene Transcription. Genetics 158: 587-596 [Abstract] [Full Text]  
  • Hall, C., Nelson, D. M., Ye, X., Baker, K., DeCaprio, J. A., Seeholzer, S., Lipinski, M., Adams, P. D. (2001). HIRA, the Human Homologue of Yeast Hir1p and Hir2p, Is a Novel Cyclin-cdk2 Substrate Whose Expression Blocks S-Phase Progression. Mol. Cell. Biol. 21: 1854-1865 [Abstract] [Full Text]  
  • Verreault, A. (2000). De novo nucleosome assembly: new pieces in an old puzzle. Genes Dev. 14: 1430-1438 [Full Text]  
  • Ridgway, P, Almouzni, G (2000). CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair. J. Cell Sci. 113: 2647-2658 [Abstract]  
  • Urnovitz, H. B., Tuite, J. J., Higashida, J. M., Murphy, W. H. (1999). RNAs in the Sera of Persian Gulf War Veterans Have Segments Homologous to Chromosome 22q11.2. CVI 6: 330-335 [Abstract] [Full Text]  
  • Farrell, M. J., Stadt, H., Wallis, K. T., Scambler, P., Hixon, R. L., Wolfe, R., Leatherbury, L., Kirby, M. L. (1999). HIRA, a DiGeorge Syndrome Candidate Gene, Is Required for Cardiac Outflow Tract Septation. Circ. Res. 84: 127-135 [Abstract] [Full Text]  
  • Richardson, R. T., Batova, I. N., Widgren, E. E., Zheng, L.-X., Whitfield, M., Marzluff, W. F., O'Rand, M. G. (2000). Characterization of the Histone H1-binding Protein, NASP, as a Cell Cycle-regulated Somatic Protein. J. Biol. Chem. 275: 30378-30386 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»