This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rudolph, C.
Right arrow Articles by Fleck, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rudolph, C.
Right arrow Articles by Fleck, O.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 1999, p. 241-250, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The msh2 Gene of Schizosaccharomyces pombe Is Involved in Mismatch Repair, Mating-Type Switching, and Meiotic Chromosome Organization

Claudia Rudolph,1 Christophe Kunz,1 Sandro Parisi,1 Elisabeth Lehmann,1 Edgar Hartsuiker,1 Berthold Fartmann,2 Wilfried Kramer,2 Jürg Kohli,1 and Oliver Fleck1,*

Institute of General Microbiology, University of Bern, CH-3012 Bern, Switzerland,1 and Institute of Molecular Genetics, Georg August University Göttingen, D-37077 Göttingen, Germany2

Received 25 June 1998/Returned for modification 4 August 1998/Accepted 9 October 1998

We have identified in the fission yeast Schizosaccharomyces pombe a MutS homolog that shows highest homology to the Msh2 subgroup. msh2 disruption gives rise to increased mitotic mutation rates and increased levels of postmeiotic segregation of genetic markers. In bandshift assays performed with msh2Delta cell extracts, a general mismatch-binding activity is absent. By complementation assays, we showed that S. pombe msh2 is allelic with the previously identified swi8 and mut3 genes, which are involved in mating-type switching. The swi8-137 mutant has a mutation in the msh2 gene which causes a truncated Msh2 peptide lacking a putative DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for correct termination of copy synthesis during mating-type switching as well as for proper organization of chromosomes during meiosis.

* Corresponding author. Mailing address: Institute of General Microbiology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland. Phone: (41) 31 631 4656. Fax: (41) 31 631 4684. E-mail: fleck{at}imb.unibe.ch.

Molecular and Cellular Biology, January 1999, p. 241-250, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gao, J., Davidson, M. K., Wahls, W. P. (2008). Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response. Nucleic Acids Res 36: 2838-2851 [Abstract] [Full Text]  
  • Yamada-Inagawa, T., Klar, A. J. S., Dalgaard, J. Z. (2007). Schizosaccharomyces pombe Switches Mating Type by the Synthesis-Dependent Strand-Annealing Mechanism. Genetics 177: 255-265 [Abstract] [Full Text]  
  • Trouiller, B., Schaefer, D. G., Charlot, F., Nogue, F. (2006). MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens. Nucleic Acids Res 34: 232-242 [Abstract] [Full Text]  
  • Holmberg, C., Fleck, O., Hansen, H. A., Liu, C., Slaaby, R., Carr, A. M., Nielsen, O. (2005). Ddb1 controls genome stability and meiosis in fission yeast. Genes Dev. 19: 853-862 [Abstract] [Full Text]  
  • Marti, T. M., Kunz, C., Fleck, O. (2003). Repair of Damaged and Mismatched DNA by the XPC Homologues Rhp41 and Rhp42 of Fission Yeast. Genetics 164: 457-467 [Abstract] [Full Text]  
  • Davis, L., Smith, G. R. (2001). Meiotic recombination and chromosome segregation in Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 98: 8395-8402 [Abstract] [Full Text]  
  • Tornier, C., Bessone, S., Varlet, I., Rudolph, C., Darmon, M., Fleck, O. (2001). Requirement for Msh6, but Not for Swi4 (Msh3), in Msh2-Dependent Repair of Base-Base Mismatches and Mononucleotide Loops in Schizosaccharomyces pombe. Genetics 158: 65-75 [Abstract] [Full Text]  
  • Mansour, A. A., Tornier, C., Lehmann, E., Darmon, M., Fleck, O. (2001). Control of GT Repeat Stability in Schizosaccharomyces pombe by Mismatch Repair Factors. Genetics 158: 77-85 [Abstract] [Full Text]  
  • Laging, M., Fartmann, B., Kramer, W. (2001). Isolation of segments of homologous genes with only one conserved amino acid region via PCR. Nucleic Acids Res 29: e8-e8 [Abstract] [Full Text]  
  • Perego, P., Jimenez, G. S., Gatti, L., Howell, S. B., Zunino, F. (2000). Yeast Mutants As a Model System for Identification of Determinants of Chemosensitivity. Pharmacol. Rev. 52: 477-492 [Abstract] [Full Text]  
  • Evans, E., Alani, E. (2000). Roles for Mismatch Repair Factors in Regulating Genetic Recombination. Mol. Cell. Biol. 20: 7839-7844 [Full Text]  
  • Hohl, M., Christensen, O., Kunz, C., Naegeli, H., Fleck, O. (2001). Binding and Repair of Mismatched DNA Mediated by Rhp14, the Fission Yeast Homologue of Human XPA. J. Biol. Chem. 276: 30766-30772 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»