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Molecular and Cellular Biology, January 1999, p. 241-250, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
The msh2 Gene of
Schizosaccharomyces pombe Is Involved in Mismatch Repair,
Mating-Type Switching, and Meiotic Chromosome Organization
Claudia
Rudolph,1
Christophe
Kunz,1
Sandro
Parisi,1
Elisabeth
Lehmann,1
Edgar
Hartsuiker,1
Berthold
Fartmann,2
Wilfried
Kramer,2
Jürg
Kohli,1 and
Oliver
Fleck1,*
Institute of General Microbiology, University
of Bern, CH-3012 Bern, Switzerland,1 and
Institute of Molecular Genetics, Georg August University
Göttingen, D-37077 Göttingen, Germany2
Received 25 June 1998/Returned for modification 4 August
1998/Accepted 9 October 1998
We have identified in the fission yeast Schizosaccharomyces
pombe a MutS homolog that shows highest homology to the Msh2
subgroup. msh2 disruption gives rise to increased mitotic
mutation rates and increased levels of postmeiotic segregation of
genetic markers. In bandshift assays performed with msh2
cell extracts, a general mismatch-binding activity is absent. By
complementation assays, we showed that S. pombe msh2 is
allelic with the previously identified swi8 and
mut3 genes, which are involved in mating-type switching. The swi8-137 mutant has a mutation in the msh2
gene which causes a truncated Msh2 peptide lacking a putative
DNA-binding domain. Cytological analysis revealed that during meiotic
prophase of msh2-defective cells, chromosomal structures
were frequently formed; such structures are rarely found in the wild
type. Our data show that besides having a function in mismatch repair,
S. pombe msh2 is required for correct termination of copy
synthesis during mating-type switching as well as for proper
organization of chromosomes during meiosis.
*
Corresponding author. Mailing address: Institute of
General Microbiology, University of Bern, Baltzerstrasse 4, CH-3012
Bern, Switzerland. Phone: (41) 31 631 4656. Fax: (41) 31 631 4684. E-mail: fleck{at}imb.unibe.ch.
Molecular and Cellular Biology, January 1999, p. 241-250, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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