Previous Article | Next Article ![]()
Molecular and Cellular Biology, January 1999, p. 261-273, Vol. 19, No. 1
Department of Molecular and Cellular Biology,
Harvard University, Cambridge, Massachusetts 02138
Received 29 July 1998/Returned for modification 16 September
1998/Accepted 28 September 1998
We have identified multiple distinct splicing enhancer elements
within protein-coding sequences of the constitutively spliced human
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Multiple Distinct Splicing Enhancers in the
Protein-Coding Sequences of a Constitutively Spliced Pre-mRNA
-globin pre-mRNA. Each of these highly conserved sequences is
sufficient to activate the splicing of a heterologous
enhancer-dependent pre-mRNA. One of these enhancers is activated by and
binds to the SR protein SC35, whereas at least two others are activated by the SR protein SF2/ASF. A single base mutation within another enhancer element inactivates the enhancer but does not change the
encoded amino acid. Thus, overlapping protein coding and RNA recognition elements may be coselected during evolution. These studies
provide the first direct evidence that SR protein-specific splicing
enhancers are located within the coding regions of constitutively spliced pre-mRNAs. We propose that these enhancers function as multisite splicing enhancers to specify 3' splice-site selection.
*
Corresponding author. Mailing address: Department of
Molecular and Cellular Biology, Harvard University, 7 Divinity Ave., Cambridge, MA 02138. Phone: (617) 495-1811. Fax: (617) 495-3537. E-mail: maniatis{at}biohp.harvard.edu.
This article has been cited by other articles:
| J. Bacteriol. | J. Virol. | Eukaryot. Cell |
|---|
| Microbiol. Mol. Biol. Rev. | Clin. Vaccine Immunol. | All ASM Journals |
|---|